[1]庞欣,陈军,李林芝,等.基于转录组测序的不同辣度辣椒差异表达基因分析[J].江苏农业科学,2025,53(6):24-33.
Pang Xin,et al.Analysis of differentially expressed genes between two peppers with different pungency degree based on transcriptome sequencing[J].Jiangsu Agricultural Sciences,2025,53(6):24-33.
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基于转录组测序的不同辣度辣椒差异表达基因分析(PDF)
Pang Xin,et al.Analysis of differentially expressed genes between two peppers with different pungency degree based on transcriptome sequencing[J].Jiangsu Agricultural Sciences,2025,53(6):24-33.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第53卷
- 期数:
- 2025年第6期
- 页码:
- 24-33
- 栏目:
- 生物技术
- 出版日期:
- 2025-03-20
文章信息/Info
- Title:
- Analysis of differentially expressed genes between two peppers with different pungency degree based on transcriptome sequencing
- Keywords:
- -
- 分类号:
- S641.301
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为了研究不同辣度辣椒的基因表达差异，探究参与或调控辣椒素生物合成的基因或转录因子。试验以2种不同辣度的辣椒B1-2(高辣)、D50(微辣)为材料，通过高通量RNA测序(RNA-seq)对35 d胎座进行转录组分析，共筛选出1 570个差异表达基因(DEG)，包括673个上调基因、897个下调基因。经GO富集分析，DEG主要集中在氧化还原反应、光合作用、光反应等生物过程；经KEGG富集分析，DEG主要富集在光合作用-天线蛋白、次级代谢产物的生物合成、代谢途径等信号通路。鉴定出7个CapCyc模型基因、8个MYB转录因子在2份辣椒材料中的差异表达；在果实不同发育时期进一步利用qRT-PCR进行分析，发现4CL、COMT、KAS可能具有调控辣椒素合成的作用。研究结果可为探索辣椒素生物合成的调控网络提供更多的候选基因，期待可为下一步关键基因的功能分析和辣椒育种提供理论基础。
- Abstract:
- -
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备注/Memo
- 备注/Memo:
-
收稿日期：2024-03-01
基因项目：国家自然科学基金(编号：32100302、32260769、31960601)；2022年江苏省高校“青蓝工程”中青年学术带头人项目；内蒙古农牧业青年创新基金(编号：2023QNJJN03)；乌兰察布市基础研究项目(编号：2021JC202)；内蒙古自然科学基金(编号：2021MS03106、2021MS03071)。
作者简介：庞欣(1985—)，女，黑龙江哈尔滨人，博士，副教授，主要从事辣椒分子生物学研究。E-mail：pxtracy916@163.com。
通信作者：黄文娟，副研究员，主要从事辣椒种质资源的鉴定及基因挖掘研究。E-mail：huangwenjuan_013@163.com。
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