|本期目录/Table of Contents|

[1]李菊,李志,马燕勤,等.基于UPLC-MS/MS解析不同樱桃番茄品种初生代谢物特征[J].江苏农业科学,2025,53(6):216-225.
 Li Ju,et al.Analysis of primary metabolites characteristics among different cherry tomato varieties based on UPLC-MS/MS[J].Jiangsu Agricultural Sciences,2025,53(6):216-225.
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基于UPLC-MS/MS解析不同樱桃番茄品种初生代谢物特征(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第6期
页码:
216-225
栏目:
贮藏加工与检测分析
出版日期:
2025-03-20

文章信息/Info

Title:
Analysis of primary metabolites characteristics among different cherry tomato varieties based on UPLC-MS/MS
作者:
李菊123李志123马燕勤123陈华4杨阿各5苗明军123钟建123常伟236杨亮123
1.四川省农业科学院园艺研究所/蔬菜种质与品种创新四川省重点实验室,四川成都 610066; 2.四川省蔬菜工程技术研究中心,四川彭州 611934; 3.农业农村部西南地区园艺作物生物学与种质创制重点实验室,四川成都 610066; 4.四川省米易县农业农村局,四川米易 617200; 5.四川省越西县农业农村局,四川越西 616650; 6.四川省食用菌研究所,四川成都 610066
Author(s):
Li Juet al
关键词:
樱桃番茄超高效液相色谱-串联质谱初生代谢物差异代谢物代谢通路品质评价遗传改良
Keywords:
-
分类号:
S641.201
DOI:
-
文献标志码:
A
摘要:
以樱桃番茄LBS、TC、Y2015-16为试验材料,利用超高效液相色谱-串联质谱技术(UPLC-MS/MS)对不同果色樱桃番茄品种的果肉初生代谢物成分进行深入分析,并通过主成分分析、聚类分析和正交偏最小二乘判别分析等统计方法比较其代谢物差异。结果表明,从3个樱桃番茄品种果肉中共检测出12类342种代谢物,其中正离子模式151种,负离子模式191种,主要包括酚酸类、氨基酸及其衍生物、核苷酸及其衍生物、有机酸、游离脂肪酸、糖类及醇类等类别。品种间的差异代谢物分析结果显示,LBS与TC之间有82种差异代谢物,其中25种上调,57种下调;LBS与Y2015-16之间有73种差异代谢物,其中52种上调,21种下调;Y2015-16与TC之间有77种差异代谢物,其中4种上调,73种下调。3个品种间变化倍数最大的差异代谢物为酚酸类、脂质。鉴定出4条在品种间存在显著差异的代谢通路,分别为泛醌和其他萜类醌生物合成、酪氨酸代谢、异喹啉生物碱生物合成和苯丙烷生物合成途径。研究结果可为樱桃番茄品种的品质评价和遗传改良提供理论依据。
Abstract:
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参考文献/References:

[1]冯岩,李朝平,朱龙英,等. 番茄果实可溶性固形物研究进展[J]. 分子植物育种,2022,20(15):5158-5163.
[2]Zhu G T,Wang S C,Huang Z J,et al. Rewiring of the fruit metabolome in tomato breeding[J]. Cell,2018,172(1/2):249-261.
[3]Quinet M,Angosto T,Yuste-Lisbona F J,et al. Tomato fruit development and metabolism[J]. Frontiers in Plant Science,2019,10:1554.
[4]尚乐乐,宋建文,王嘉颖,等. 番茄果实品质形成及其分子机理研究进展[J]. 中国蔬菜,2019(4):21-28.
[5]Agarwal S,Rao A V. Tomato lycopene and its role in human health and chronic diseases[J]. CMAJ,2000,163(6):739-744.
[6]何婉如,张晓熙,徐长玉,等. 黄酮类化合物防治糖尿病及其并发症研究进展[J]. 浙江中医药大学学报,2024,48(2):241-246.
[7]Tieman D,Zhu G T,Resende M F R,et al. A chemical genetic roadmap to improved tomato flavor[J]. Science,2017,355(6323):391-394.
[8]程远,万红建,姚祝平,等. 不同品种樱桃番茄氨基酸组成及风味分析[J]. 核农学报,2019,33(11):2177-2185.
[9]Pan F,Zhang Q R,Zhu H S,et al. Transcriptome and metabolome provide insights into fruit ripening of cherry tomato (Solanum lycopersicum var.cerasiforme)[J]. Plants,2023,12(19):3505.
[10]林欣,黄世安,张琴,等. 采用UPLC-MS/MS分析低温贮藏期间‘空心李’果实初生代谢物[J]. 植物生理学报,2022,58(10):1982-1994.
[11]张琴,黄世安,林欣,等. 基于UPLC-MS/MS的3个李品种果实初生代谢物分析[J]. 食品科学,2022,43(16):226-234.
[12]Onda Y,Miyagi A,Takahara K,et al. Effects of NAD kinase 2 overexpression on primary metabolite profiles in rice leaves under elevated carbon dioxide[J]. Plant Biology,2014,16(4):819-824.
[13]Antunes A C,Acunha T D S,Perin E C,et al. Untargeted metabolomics of strawberry (Fragaria×Ananassa‘Camarosa’) fruit from plants grown under osmotic stress conditions[J]. Journal of the Science of Food and Agriculture,2019,99(15):6973-6980.
[14]Zhao X D,Yuan X Y,Chen S,et al. Role of the tomato TAGL1 gene in regulating fruit metabolites elucidated using RNA sequence and metabolomics analyses[J]. PLoS One,2018,13(6):e0199083.
[15]沈央红,方金玉,朱军莉,等. 代谢组学在食品质量安全领域的应用进展[J]. 食品科学,2023,44(5):282-289.
[16]苏璐璐,朱文莹,陈旭,等. 基于转录组学和代谢组学解析番茄果实品质形成过程[J]. 山东农业科学,2023,55(2):9-19.
[17]方贤胜,吴涛,肖良俊. 基于广泛靶向代谢组学的浅黄色和紫色核桃内种皮成分差异分析[J]. 食品科学,2021,42(12):215-221.
[18]Yin Q C,Ji J B,Zhang R H,et al. Identification and verification of key taste components in wampee using widely targeted metabolomics[J]. Food Chemistry,2022,13:100261.
[19]Zou S C,Wu J C,Shahid M Q,et al. Identification of key taste components in loquat using widely targeted metabolomics[J]. Food Chemistry,2020,323:126822.
[20]郑海英,张冬青,赵晓丹. 代谢组学法研究转录因子SlNAC4对番茄果实代谢产物的影响[J]. 食品科学,2019,40(10):36-42.
[21]Guan D,Zhao Y,Zhao X D,et al. Metabolomics study of the effect of transcription factor NOR-like1 on flavonoids in tomato at different stages of maturity using UPLC-MS/MS[J]. Foods,2023,12(24):4445.
[22]Jia C P,Guo B,Wang B K,et al. Integrated metabolomic and transcriptomic analysis reveals the role of phenylpropanoid biosynthesis pathway in tomato roots during salt stress[J]. Frontiers in Plant Science,2022,13:1023696.
[23]Yang T X,Ali M,Lin L H,et al. Recoloring tomato fruit by CRISPR/Cas9-mediated multiplex gene editing[J]. Horticulture Research,2023,10(1):uhac214.
[24]王同林,叶红霞,郑积荣,等. 番茄果实中主要风味物质研究进展[J]. 浙江农业学报,2020,32(8):1513-1522.
[25]郭精桐,赵圆,孙玉敬. 番茄果实风味及其影响因素的研究进展[J]. 食品科学,2023,44(17):169-177.
[26]姚国新,陈刚,卢勇,等. 3,4-二甲氧基苯乙酸的合成新工艺[J]. 中国医药工业杂志,2010,41(10):730-731.
[27]周俊萍,徐玉娟,温靖,等. γ-氨基丁酸(GABA)的研究进展[J]. 食品工业科技,2024,45(5):393-401.
[28]霍冬敖,田瑞丰,任永权,等. 基于UPLC-MS/MS技术的野生及栽培韭菜籽的代谢组学研究[J]. 广西植物,2022,42(12):1995-2006.
[29]Pinela J,Montoya C,Carvalho A M,et al. Phenolic composition and antioxidant properties of ex-situ conserved tomato (Solanum lycopersicum L.) germplasm[J]. Food Research International,2019,125:108545.
[30]宿子文,蔡志翔,孙朦,等. 植物中绿原酸生物合成研究进展[J]. 江苏农业学报,2023,39(6):1414-1426.
[31]Klepacka J,Gujska E,Michalak J. Phenolic compounds as cultivar-and variety-distinguishing factors in some plant products[J]. Plant Foods for Human Nutrition,2011,66(1):64-69.

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备注/Memo

备注/Memo:
收稿日期:2024-06-18
基金项目:国家重点研发计划(编号:2022YFD1100205);国家现代农业产业技术体系建设专项四川特色蔬菜创新团队项目[编号:sccxtd-2024-22];四川省科技计划(编号:2021YFYZ0022、2023NSFSC0162);四川省财政自主创新专项(编号:2022ZZCX050)。
作者简介:李菊(1989—),女,重庆人,硕士,副研究员,主要从事蔬菜育种与栽培研究。E-mail:1152613043@qq.com。
通信作者:常伟,研究员,主要从事蔬菜育种与栽培研究,E-mail:471243498@qq.com;杨亮,博士,副研究员,主要从事番茄遗传育种研究,E-mail:yangliang_saas@foxmail.com。
更新日期/Last Update: 2025-03-20