|本期目录/Table of Contents|

[1]付小猛,龙春瑞,杨虹霞,等.pH值对柠檬根际土壤微生物群落结构与功能的影响[J].江苏农业科学,2025,53(6):264-271.
 Fu Xiaomeng,et al.Impacts of pH value on structure and function of microbial community in lemon rhizosphere soil[J].Jiangsu Agricultural Sciences,2025,53(6):264-271.
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pH值对柠檬根际土壤微生物群落结构与功能的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第6期
页码:
264-271
栏目:
资源与环境
出版日期:
2025-03-20

文章信息/Info

Title:
Impacts of pH value on structure and function of microbial community in lemon rhizosphere soil
作者:
付小猛龙春瑞杨虹霞刘红明毛加梅杜玉霞周先艳魏舒娅彭忠良郭莉娜岳建强
云南省农业科学院热带亚热带经济作物研究所,云南保山 678000
Author(s):
Fu Xiaomenget al
关键词:
柠檬pH值宏基因组测序土壤微生物土壤酶活
Keywords:
-
分类号:
S666.506+.1
DOI:
-
文献标志码:
A
摘要:
以2年生盆栽柠檬为研究对象,采用Illumina测序平台进行宏基因组高通量测序,分析不同pH值处理下土壤微生物群落的结构差异,并预测土壤微生物的功能特征。结果表明,酸性(PTLB)处理的柠檬根际土壤微生物多样性明显高于碱性(PTLD)、强碱(PTLE)处理,且物种组成结构差异显著。本研究基于宏基因组学测序得到的物种,隶属于47门116纲221目416科977属3 638种(除去未鉴定微生物),各处理的主要优势菌门均为假单胞菌门、放线菌门、热变形菌门。在属分类水平下对物种绝对丰度排名前20位的菌属进行聚类分析,发现强酸(PTLA)、酸性(PTLB)处理的微生物群落组成更相似,中性(PTLC)、碱性(PTLD)、强碱(PTLE)处理的微生物群落组成更相似,且大部分物种在PTLA、PTLB处理下的相对丰度要高于PTLC、PTLD、PTLE处理。基于EggNOG数据库注释,各处理参与翻译、核糖体结构和生物合成、能量生成与转化、氨基酸转运和代谢的功能基因的相对丰度较高,且PTLB处理中参与氨基酸转运和代谢、能量生成和转化的功能基因的相对丰度显著高于其他处理。相关性分析表明,5个环境因子(土壤脲酶、蔗糖酶、纤维素酶、过氧化氢酶、处理液pH值)对土壤微生物的群落结构和功能均有显著影响,其中处理液pH值的影响最为突出。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-04-16
基金项目:国家重点研发计划(编号:2022YFF1302404);现代农业(柑橘)产业技术体系建设专项(编号:CARS-26);云南省科技厅农业联合专项(编号:202101BD070001-036);云南省科技厅重大科技专项(编号:202102AE090054)。
作者简介:付小猛(1986—),女,云南保山人,硕士,助理研究员,主要研究方向为土壤质量提升与微生物。E-mail:274772199@qq.com。
通信作者:岳建强,硕士,研究员,研究方向为柠檬栽培技术。E-mail:yjq7009@163.com。
更新日期/Last Update: 2025-03-20