|本期目录/Table of Contents|

[1]傅子森,李盼禹,陈靖.高效降解纤维素产甲烷复合菌群的富集和群落结构解析[J].江苏农业科学,2025,53(6):272-280.
　Fu Zisen,et al.Enrichment and community structure of efficient cellulose-degrading multiple microorganisms with methanogenesis[J].Jiangsu Agricultural Sciences,2025,53(6):272-280.
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高效降解纤维素产甲烷复合菌群的富集和群落结构解析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第6期
页码:
272-280
栏目:
资源与环境
出版日期:
2025-03-20

文章信息/Info

Title:
Enrichment and community structure of efficient cellulose-degrading multiple microorganisms with methanogenesis
作者:
傅子森; 李盼禹; 陈靖
四川大学化学工程学院，四川成都 610000
Author(s):
Fu Zisen; et al
关键词:
纤维素; 厌氧消化; 高效降解菌群; 富集培养; 环境菌群鉴定
Keywords:
-
分类号:
X172
DOI:
-
文献标志码:
A
摘要:
纤维素是农业秸秆的主要组成部分。对降解纤维素产甲烷功能菌群的研究不够深入和缺乏高效的功能菌群，极大地制约了循环资源化利用农业秸秆来生产生物燃气的技术工艺开发。为深入解析能够高效降解纤维素产甲烷的复合菌群，实现农业废弃物的资源化利用，采用亨盖特厌氧管技术从多个环境样品中富集和筛选能够厌氧消化纤维素产甲烷的复合菌群，利用气相色谱法测定富集管中甲烷含量和挥发性脂肪酸盐含量，测定复合菌群在不同添加浓度下的纤维素降解率，以评估复合菌群产甲烷性能和纤维素降解能力。通过高通量测序技术解析不同环境样品中的微生物群落结构。结果表明，从3个环境样品中成功富集培养出能够降解纤维素产甲烷的复合菌群，甲烷含量最高可占气体产出的92.2%，挥发性脂肪酸得到有效利用，只添加了乙酸盐的样品LD-1在厌氧消化过程中的挥发性脂肪酸盐含量降低到726.4 mg/L。3个环境样品富集得到的复合菌群对纤维素均表现出较高的降解能力，纤维素添加量为10 g/L时，最高降解率可达32.1%，50 g/L纤维素添加量下纤维素最高降解量为9.05 g/L。细菌分类上厚壁菌门和变形菌门是主要优势菌门，古菌分类上广古菌门和Halobacterota是主要的优势门。在细菌属水平上，发挥关键功能的菌群主要有假单胞菌属、芽孢杆菌属、Mobilitalea、梭状芽孢杆菌属和醋弧菌属等；在古菌属水平上，厌氧消化过程中发挥关键功能的菌群主要有甲烷袋状菌属、甲烷杆菌属、Methanoregula、鬃毛甲烷菌属和甲烷八叠球菌属。研究结果有助于科学构建高效的复合菌剂，助力农业秸秆的循环资源化利用。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2024-03-31
基金项目：国家自然科学基金面上项目(编号：32270124)；四川省科技厅重点研发项目(编号：2021YFS0292)；成都市科技局技术研发创新项目(编号：2019-YF05-01962-SN)。
作者简介：傅子森(1999—)，男，海南海口人，硕士研究生，主要研究方向为低碳生物技术、生物质资源转化、微生物资源利用。E-mail：fzs0209@qq.com。
通信作者：陈靖，博士，副教授，主要研究方向为绿色生物化工、环境生物技术、重金属污染生物修复、低碳生物技术等。E-mail：jing.chen@scu.edu.cn。
更新日期/Last Update: 2025-03-20