[1]但佳鑫,何芷慧,柳志强,等.禾谷炭疽菌C6型转录因子Cgrcdf1的生物学功能[J].江苏农业科学,2026,54(11):118-126.
 Dan Jiaxin,et al.Biological function of a C6type transcription factor Cgrcdf1 in Colletotrichum graminicola[J].Jiangsu Agricultural Sciences,2026,54(11):118-126.
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禾谷炭疽菌C6型转录因子Cgrcdf1的生物学功能()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第11期
页码:
118-126
栏目:
植物保护
出版日期:
2026-06-05

文章信息/Info

Title:
Biological function of a C6type transcription factor Cgrcdf1 in Colletotrichum graminicola
作者:
但佳鑫 何芷慧 柳志强 李晓宇
海南大学生命健康学院,海南海口 570228
Author(s):
Dan Jiaxinet al
关键词:
禾谷炭疽菌C6转录因子营养生长分生孢子致病性
Keywords:
-
分类号:
S435.131.4+9
DOI:
-
文献标志码:
A
摘要:
禾谷炭疽菌(Colletotrichum graminicola)作为侵害玉米的关键病原菌之一,每年会引发严重的经济损失,对全球粮食安全构成了严峻的威胁。在真菌的生长发育进程中,C6型转录因子发挥着至关重要的作用。为了解禾谷炭疽菌C6型转录因子Cgrcdf1的生物学功能并为新型防控策略的制定提供理论依据,本研究采用同源重组技术成功构建了Cgrcdf1的敲除突变株,并进行表型分析,包括亚细胞定位、菌丝生长状况、孢子产生与萌发、附着枝形成以及致病性等多个方面。结果表明,Cgrcdf1编码1个含有694个氨基酸的蛋白质,含有1个C6锌指结构域和1个Fungal_trans结构域。Cgrcdf1敲除突变株表现为营养生长减慢,在PDA培养基上,突变株的平均菌落直径(55 mm)显著小于野生型及互补菌株(平均68 mm),无法形成分生孢子梗,同时不产生镰刀形孢子,卵圆形孢子产量减少,萌发率大幅降低,在保湿培养6 h后野生型菌株萌发率接近95%,而突变株只有5%,附着枝生长延缓,致病力减弱。综上所述,Cgrcdf1是禾谷炭疽菌中一个重要的C6型转录因子,其功能缺失会影响菌体的营养生长、产孢与萌发、附着枝与孢子梗发育以及致病性等多个方面。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2025-11-03
基金项目:国家自然科学基金(编号:32560351)。
作者简介:但佳鑫(1998—),男,四川成都人,硕士,主要从事植物病原真菌学研究。E-mail:2906303980@qq.com。
通信作者:李晓宇,博士,教授,主要从事真菌生物学研究。E-mail:lixiaoyu@hainanu.edu.cn。
更新日期/Last Update: 2026-06-05