[1]王天放,陈泽斌,林丽,等.化肥减施条件下深翻与生物炭对烤烟连作土壤酚酸含量和微生物多样性的调节[J].江苏农业科学,2026,54(11):245-251.
　Wang Tianfang,et al.Regulation effects of deep tillage and biochar on phenolic acid content and microbial diversity in continuous cropping soil of fluecured tobacco under condition of reduced fertilizer application[J].Jiangsu Agricultural Sciences,2026,54(11):245-251.
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化肥减施条件下深翻与生物炭对烤烟连作土壤酚酸含量和微生物多样性的调节()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第11期
页码:
245-251
栏目:
农业资源与环境
出版日期:
2026-06-05

文章信息/Info

Title:
Regulation effects of deep tillage and biochar on phenolic acid content and microbial diversity in continuous cropping soil of fluecured tobacco under condition of reduced fertilizer application
作者:
王天放1;  陈泽斌1;  林丽1;  徐胜光1; 2;  范志伟1;  魏薇1;  朱再香1;  闫悦1;  李清梅1
1.昆明学院农学与生命科学学院，云南昆明 650214； 2.云南省高校生物炭工程研究中心，云南昆明 650214
Author(s):
Wang Tianfang; et al
关键词:
化肥减施; 深翻; 生物炭; 烟草; 连作障碍; 酚酸; 微生物多样性
Keywords:
-
分类号:
S572.06
DOI:
-
文献标志码:
A
摘要:
为了研究化肥减施条件下深翻与生物炭对烤烟连作土壤酚酸含量及微生物多样性的调节作用，以揭示其缓解连作障碍的机理，在云南省宣威市连续7年烤烟连作地块设置8个田间处理，包括深翻与否、化肥减施(75%、50%)及生物炭配施(300 g/株)，通过高效液相色谱(HPLC)测定土壤酚酸含量，通过Biolog-ECO板技术分析微生物多样性。结果表明，深翻或化肥减施均显著降低土壤中的4种酚酸含量，其中深翻+75%化肥(B2处理)效果最佳，酚酸总量较对照(A1)降低80%以上。B2处理下微生物碳源利用能力(AWCD)和Shannon多样性指数最高，分别为0.72和3.33，优于其他处理。生物炭配施(B3)反而增加酚酸含量并降低微生物多样性。相关性分析表明，酚酸含量与微生物Shannon指数、优势度指数极显著负相关(P<0.01)。综上，深翻结合75%化肥减施可有效消减酚酸积累并提升微生物多样性，是缓解烤烟连作障碍的最优措施；生物炭在特定条件下可能加剧酚酸胁迫，该研究为实际生产中防控连作障碍提供了理论依据和技术参考。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-04-29
基金项目：云南省地方本科高校基础研究联合专项(编号：202401BA070001-004)；国家自然科学基金(编号：42067009)；云南省科技计划重大科技专项(编号：202202AE090015)；云南省教育厅科学研究基金(编号：2024Y742、2023Y0863)；云南省教育厅2023年大学生创新训练计划(编号：S202311393044、S202311393061)。
作者简介：王天放(1996—)，男，辽宁阜新人，硕士研究生，主要从事烟草连作障碍消减技术及机理研究，E-mail：401013540@qq.com；共同第一作者：林丽(1976—)，女，云南水富人，硕士，讲师，主要从事植物保护与微生物研究，E-mail：linli@kmu.edu.cn。
通信作者：陈泽斌，博士，教授，主要从事烟草连作障碍成因及消减技术研究，E-mail：zbchenkmu@163.com。
更新日期/Last Update: 2026-06-05