[1]李陈红,邱陈雨,王涛,等.糯高粱根际溶磷细菌的筛选鉴定与功能活性[J].江苏农业科学,2026,54(11):259-266.
 Li Chenhong,et al.Screening,identification and functional characteristics of phosphorus solubilizing bacteria isolated from rhizosphere of glutinous sorghum[J].Jiangsu Agricultural Sciences,2026,54(11):259-266.
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糯高粱根际溶磷细菌的筛选鉴定与功能活性()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第11期
页码:
259-266
栏目:
农业资源与环境
出版日期:
2026-06-05

文章信息/Info

Title:
Screening,identification and functional characteristics of phosphorus solubilizing bacteria isolated from rhizosphere of glutinous sorghum
作者:
李陈红 邱陈雨 王涛 张永光
宜宾学院农林与食品工程学部,四川宜宾 644000
Author(s):
Li Chenhonget al
关键词:
糯高粱溶磷细菌溶磷特性促生活性多样性功能活性
Keywords:
-
分类号:
S182;S514.01
DOI:
-
文献标志码:
A
摘要:
为了解糯高粱根际溶磷细菌多样性及其功能属性,从宜宾糯高粱根际采集的14份土样中分离溶磷菌,对具有明显溶磷活性的菌株进行16S rRNA基因序列分析,测定其溶磷、固氮,产吲哚-3-乙酸,脲酶、蛋白酶、淀粉酶、纤维素酶和脂肪酶的功能活性。结果表明,具有明显溶磷活性的27株菌分布在3个门、5个纲、9个目、11个科和15个属,变形菌门(Proteobacteria)为优势门(占总菌株数的66.7%),伯克霍尔德氏菌属(Burkholderia)为优势属(占总菌株数的18.5%);不同菌株对植酸钙、卵磷脂、磷酸钙、磷酸铝和磷酸铁的水解活性差异较大,其中对磷酸钙和植酸钙的最高水解活性分别为(203.6±7.5) mg/L和(101.9±3.6) mg/L,对磷酸铁的水解活性最低;4株菌产吲哚3-乙酸浓度超过100 mg/L,最高浓度为(483.3±13.7) mg/L;具固氮、脲酶和蛋白酶活性菌株的比例分别为40.7%、18.5%和259%;纤维素水解活性阳性率为100%,但活性普遍较低,14.8%和约51.9%的菌株淀粉酶和脂肪酶活性检测为阳性。综上,糯高粱根际可培养的溶磷细菌物种丰富,部分菌株还具有固氮、产吲哚3-乙酸及多种水解酶的功能活性,是促生菌剂开发的重要资源。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2025-09-19
基金项目:四川省高等学校创新性实验项目(编号:SCXS202401);宜宾学院科学研究基金(编号:2020YY01);宜宾学院博士启动基金(编号:2020QH04)。
作者简介:李陈红(1974—),女,四川新都人,高级农艺师,主要从事根际微生物与植物互作研究。E-mail:zixuan_xj@163.com。
通信作者:张永光,博士,副研究员,研究方向为特殊环境微生物资源挖掘与利用。E-mail:ygz0966@hotmail.com。
更新日期/Last Update: 2026-06-05