[1]孙婧陶,李兆华,张宝修,等.过氧化氢诱导延边奶山羊乳腺上皮细胞氧化损伤模型的建立[J].江苏农业科学,2013,41(10):149-152.
　Sun Jingtao,et al.Oxidative damage model establishment of mammary epithelial cells from Yanbian dairy goats induced by hydrogen peroxide[J].Jiangsu Agricultural Sciences,2013,41(10):149-152.
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过氧化氢诱导延边奶山羊乳腺上皮细胞
氧化损伤模型的建立()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年10期
页码:
149-152
栏目:
畜牧兽医
出版日期:
2013-10-25

文章信息/Info

Title:
Oxidative damage model establishment of mammary epithelial cells from Yanbian dairy goats induced by hydrogen peroxide
作者:
孙婧陶1; 李兆华2; 张宝修1; 茹李波1; 李钟淑1; 方南洙1
1.延边大学农学院/动物遗传育种与繁殖实验室，吉林延吉 133002； 2.吉林省农业科学院，吉林长春 130033
Author(s):
Sun Jingtao; et al
关键词:
延边奶山羊; H2O2; 乳腺上皮细胞; 氧化损伤; 凋亡
Keywords:
-
分类号:
S852.2
DOI:
-
文献标志码:
A
摘要:
为了建立过氧化氢诱导延边奶山羊乳腺上皮细胞(DGMECs)氧化损伤模型，用体外培养的延边奶山羊乳腺上皮细胞传代后进行试验，用DMEM/F12培养基将H2O2稀释为5、50、100、200、500 μmol/L，以DMEM/F12培养基作为空白对照组。以H2O2处理4 h后，用MTT法观察H2O2对乳腺上皮细胞活力的影响，用Hoechst33342/PI荧光染色法观察H2O2损伤对乳腺上皮细胞的促凋亡情况，DCFH-DA检测细胞内活性氧(ROS)含量。MTT活性检测结果表明，各处理组的细胞存活率与未处理组差异显著(P＜0.05)。5、50、100 μmol/L H2O2作用4 h后，细胞存活率分别为91.21、77.50%、54.48%；而200、500 μmol/L H2O2作用4 h后，细胞存活率仅为35.53%、27.79%，乳腺上皮细胞死亡过多。Hoechst33342/PI染色结果表明，H2O2通过诱导乳腺上皮细胞的凋亡及坏死来降低细胞的存活率。利用荧光探针DCFH-DA分析DGMECs的ROS水平，结果显示，细胞经H2O2处理后会明显增加细胞内ROS水平。H2O2 100 μmol/L 处理细胞4 h后，约导致50%的细胞死亡，坏死比例小，ROS含量也相对较少，在此浓度下，细胞还具有恢复其功能的可能性，因此选用100 μmol/L H2O2处理细胞4 h来建立延边奶山羊乳腺上皮细胞氧化损伤模型。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2013-06-30
基金项目：国家自然科学基金(编号：3136546)；吉林省科技厅重点项目(编号：20100228)。
作者简介：孙婧陶(1990—)，女，河北承德人，硕士研究生，研究方向为动物繁殖与生物技术。E-mail：sunjt1990@126.com。
通信作者：方南洙(1960—)，男，朝鲜族，博士，教授，研究方向为动物繁殖与生物技术。E-mail：nzfang@ybu.edu.cn。
更新日期/Last Update: 2013-10-25