[1]梁文杰.梨S22-RNase基因全长cDNA克隆及序列分析[J].江苏农业科学,2013,41(12):34-37.
　Liang Wenjie.Full-length cDNA cloning and sequence analysis of S22-RNase gene from pears[J].Jiangsu Agricultural Sciences,2013,41(12):34-37.
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梨S22-RNase基因全长cDNA克隆及序列分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年12期
页码:
34-37
栏目:
生物技术
出版日期:
2013-12-25

文章信息/Info

Title:
Full-length cDNA cloning and sequence analysis of S22-RNase gene from pears
作者:
梁文杰1; 2
1.中南林业科技大学经济林育种与栽培国家林业局重点实验室，湖南长沙 410004； 2.温州科技职业学院，浙江温州 325006
Author(s):
Liang Wenjie
关键词:
砂梨; 自交不亲和性; S基因; 克隆; 序列分析
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
S661.201
摘要:
以砂梨品种“宝珠”雌蕊为材料提取RNA，通过采用RACE技术，获得了1个840 bp梨S基因的cDNA目的片段，经克隆后测序，证实该片段是梨S22-RNase的全长cDNA序列；分析表明，该基因的编码框由681个碱基组成，共编码227个氨基酸，与已克隆的梨S-RNase基因的cDNA序列的相似性为60%～90%。
Abstract:
-

参考文献/References:

［1］Stone J L. Molecular mechanisms underlying the break down of gametophytic self-incompatibility［J］. Rev Biol，2002，77(1)：17-32.
［2］Sophia L S，Daphne R G. The molecular biology of self-incompatibility systems in flowering plants［J］. Plant Cell，Tissue and Organ Culture，2001，67(2)：93-114.
［3］Javier S，María H. Identification of self-incompatibility alleles in pear cultivars(Pyrus communis L.)［J］. Euphytica，2004，128(3)：325-331.
［4］Silvia Z，Paola T，Wim B，et al. S-Allele characterization in self-incompatible pear(Pyrus communis L.)［J］. Sex Plant Repord，2002，15(3)：153-158.
［5］Norioka N，Ohnishi Y，Norioka S，et al. Nucleotide sequences of cDNAs encoding S2-and S4-Rnases(D49527 and D49528 for EMBL)from Japanese pear(Pyrus pyrifolia Nakai)(PGR95-020)［J］. Plant Physiol，1995，108：1343.
［6］Ishimizu T，Shinkawa T，Sakiyama F，et al. Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility［J］. Plant Molecular Biology，1998，37(6)：931-941.
［7］Castillo C，Takasaki T，Saito T，et al. Cloning of the S8-RNase(S8-allele)of Japanese Pear(Pyrus pyrifolia Nakai)［J］. Plant Biotechnology，2002，19(1)：1-6.
［8］Takasaki T，Okada K，Castillo C，et al. Sequence of the S9-RNase cDNA and PCR-RFLP system for discriminating S1- to S9-allele in Japanese pear［J］. Euphytica，2004，135(2)：157-167.
［9］Hoy-Taek K，Yutaka H，Hyun-jung K，et al. The presence of a new S-RNase allele(S10)in Asian pear(Pyrus pyrifolia (Burm；Nakai))［J］. Genetic Resources and Crop Evolution，2006，53(7)：1375-1383.
［10］Raspe O，Kohn J R. Population structure at the S-locus of Sorbus aucuparia L.(Rosaceae：Maloideae)［J］. Molecular Ecology，2007，16(6)：1315-1325.
［11］Cornish E C，Pettitt J M，Boning I，et al. Developmentally controlled expression of a gene associated with self-incompatibility in Nicotiana alata［J］. Nature，1987，326：99-102.
［12］Sassa H，Nishio T，Kowyama Y，et al. Self-incompatibility(S)alleles of the rosaceae encode members of a distinct class of the T2/S ribonuclease superfamily［J］. Molecular & General Genetics，1996，250(5)：547-557.

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备注/Memo

备注/Memo:
收稿日期：2013-05-09
基金项目：国家林业局重点项目(编号：2006-12)。
作者简介：梁文杰(1981—)，男，硕士，讲师，研究方向为林业生物技术和林业碳汇。E-mail：lwj60000@126.com。
更新日期/Last Update: 2013-12-25