|本期目录/Table of Contents|

[1]韩金龙,李慧,蔺经,等.钙对盐胁迫下杜梨叶片抗氧化系统的影响[J].江苏农业科学,2016,44(06):245-248.
 Han Jinglong,et al.Effect of Ca on leaf antioxidant system of Pyrus betulaefolia under salt stress[J].Jiangsu Agricultural Sciences,2016,44(06):245-248.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年06期
页码:
245-248
栏目:
园艺与林学
出版日期:
2016-06-25

文章信息/Info

Title:
Effect of Ca on leaf antioxidant system of Pyrus betulaefolia under salt stress
作者:
韩金龙12 李慧1 蔺经1 杨青松1 常有宏1
1.江苏省农业科学院园艺研究所/江苏省高效园艺作物遗传改良重点实验室,江苏南京 210014;
2.南京农业大学园艺学院,江苏南京 210095
Author(s):
Han Jinglonget al
关键词:
杜梨盐胁迫活性氧抗氧化酶
Keywords:
-
分类号:
S661.201
DOI:
-
文献标志码:
A
摘要:
为探讨外源钙对盐胁迫下杜梨叶片抗氧化系统的影响,以梨砧木杜梨幼苗为供试材料,在水培条件下,研究外源施加不同浓度的钙对200 mmol/L NaCl胁迫下其叶片抗氧化酶活性、活性氧产生、膜质过氧化和抗氧化物质含量的影响。结果显示:NaCl胁迫3 d后,杜梨叶片中超氧化物歧化酶(SOD)活性减弱,过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)、谷胱甘肽过氧化物酶(GSH-Px)、抗坏血酸过氧化物酶(APX)的活性增强,活性氧(O-2[KG-*2]· 、H2O2)和丙二醛(MDA)大量积累,抗氧化物质谷胱甘肽(GSH)和抗坏血酸(AsA)合成下降。施加外源CaCl2能增强NaCl胁迫下杜梨叶片中SOD、POD、CAT、GR、GSH-Px、APX的活性,减少O-2[KG-*2]· 和H2O2的产生,降低脂质过氧化程度,提高GSH和AsA的含量,有效缓解盐胁迫对杜梨叶片的过氧化伤害,其中以10 μmol/L CaCl2处理效果最为显著。说明NaCl处理可对杜梨叶片造成氧化伤害,施加低浓度(5~10 μmol/L)CaCl2处理可缓解盐胁迫对杜梨叶片抗氧化系统的影响。
Abstract:
-

参考文献/References:

[1]章文华. 植物的抗盐生理和盐害的防治[J]. 植物生理学通讯,1997,33(6):479.
[2]Parida A K,Das A B. Salt tolerance and salinity effects on plants:a review[J]. Ecotoxicology and Environmental Safety,2005,60(3):324-349.
[3]Braam J,Davis R W. Rain-,wind-,and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis[J]. Cell,1990,60(3):357-364.
[4]张燕,方力,李天飞,等. 钙对低温胁迫的烟草幼苗某些酶活性的影响[J]. 植物学通报,2002,19(3):342-347.
[5]张建霞,李新国,孙中海. 外源钙对柑橘抗热性的相关生理生化指标的影响[J]. 华中农业大学学报,2005,24(4):397-400.
[6]朱晓军,梁永超,杨劲松,等. 钙对盐胁迫下水稻幼苗抗氧化酶活性和膜脂过氧化作用的影响[J]. 土壤学报,2005,42(3):453-459.
[7]Okubo M,Sakuratani T. Effects of Sodium chloride on survival and stem elongation of two Asian pear rootstock seedlings[J]. Scientia Horticulturae,2000,85(1/2):85-90.
[8]Matsumoto K,Tamura F,Chun J P,et al. Enhancement in salt tolerance of Japanese pear by using Pyrus betulaefolia rootstock[J]. Hortic Res,2007,6(1):47-52.
[9]Okubo M,Furukawa Y,Sakuratani T. Growth,flowering and leaf properties of pear cultivars grafted on two Asian pear rootstock see dlings under NaCl irrigation[J]. Scientia Horticulturae,2000,85(1/2):91-101.
[10]Matsumoto K,Chun J P,Tamura F,et al. Salt tolerance in Pyrus species is linked to levels of Na and Cl translocation from roots to leaves[J]. Journal of the Japanese Society for Horticultural Science,2006,75(5):385-391.
[11]Wu Q S,Zou Y N. Adaptive responses of birch-leaved pear (Pyrus betulaefolia) seedlings to salinity stress[J]. Notulae Botanicae Horti Agrobotanici Cluj:Napoca,2009,37(1):133-138.
[12]Patterson B D,Macrae E A,Ferguson I B. Estimation of hydrogen peroxide in plant extracts using titanium(Ⅳ)[J]. Analytical Biochemistry,1984,139(2):487-492.
[13]Elstner E F,Heupel A. Inhibition of nitrite formation from hydroxylammoniumchloride:a simple assay for superoxide dismutase[J]. Analytical Biochemistry,1976,70(2):616-620.
[14]李合生. 植物生理学实验技术指导[M]. 北京:高等教育出版社,2001.
[15]Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(1/2):248-254. [16]Ma C,Wang Z Q,Kong B B,et al. Exogenous trehalose differentially modulate antioxidant defense system in wheat callus during water deficit and subsequent recovery[J]. Plant Growth Regulation,2013,70(3):275-285.
[17]Aebi H. Catalase in vitro[J]. Methods in Enzymology,1984,105:121-126.
[18]Ali B,Tao Q J,Zhou Y F,et al. 5-Aminolevolinic acid mitigates the cadmium-induced changes in Brassica napus as revealed by the biochemical and ultra-structural evaluation of roots[J]. Ecotoxicology and Environmental Safety,2013,92:271-280.
[19]黄爱缨,吴珍龄. 水稻谷胱甘肽过氧化物酶的测定法[J]. 西南农业大学学报,1999,21(4):24-27.
[20]Nakano Y,Asada K. Hydrogen peroxide is scanvenged by ascorbated specific peroxidase in spinach chloroplasts[J]. Plant & Cell Physiology,1981,22(5):867-880.
[21]Anderson M E. Determination of glutathione and glutathione disulfide in biological samples[J]. Methods in Enzymology,1985,113:548-555.
[22]Law M Y,Charles S A,Halliwell B.Glutathion and scorbic acid in spinach (Spinacia oleracea) chloroplast.The effect of hydrogen peroxide and of paraquat[J]. Journal of Biochemistry,1983,253:109-116.
[23]Cramer G R,Luchli A,Polito V S. Displacement of Ca by Na from the plasmalemma of root cells:a primary response to salt stress[J]. Plant Physiology,1985,79(1):207-211.
[24]Lynch J,Polito V S,Luchli A. Salinity stress increases cytoplasmic ca activity in maize root protoplasts[J]. Plant Physiology,1989,90(4):1271-1274.
[25]Ben A N,Megdiche W,Jiménez A,et al. The effect of calcium on the antioxidant systems in the halophyte Cakile maritima under salt stress[J]. Acta Physiologiae Plantarum,2010,32(3):453-461.
[26]周焱. Ca2+对水稻幼苗生长的影响[J]. 贵州师范大学学报:自然科学版,2002,20(3):12-14.
[27]王勋陵,陈鑫阳,鲁晓云. 钙对臭氧伤害小麦的防护作用[J]. 西北植物学报,1993,13(3):163-169.

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备注/Memo

备注/Memo:
收稿日期:2015-04-23
基金项目:国家自然科学基金(编号:31372051);江苏省农业科技自主创新资金[编号:CX(14)5018]。
作者简介:韩金龙(1989—),男,山西霍州人,硕士研究生,主要从事果树逆境生理和分子生物学研究。E-mail:hjlong24@126.com。
通信作者:常有宏,研究员,硕士生导师,主要从事果树育种和栽培生理研究。E-mail:cyh@jaas.ac.cn。
更新日期/Last Update: 2016-06-25