[1]李晓筱,徐俊雄,刘婵,等.虎杖MYB转录因子PcMYB2基因的克隆与原核表达[J].江苏农业科学,2018,46(23):50-55.
　Li Xiaoxiao,et al.Cloning and prokaryotic expression of MYB transcription factor PcMYB2 gene from Polygonum cuspidatum[J].Jiangsu Agricultural Sciences,2018,46(23):50-55.
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虎杖MYB转录因子PcMYB2基因的克隆与原核表达()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年第23期
页码:
50-55
栏目:
生物技术
出版日期:
2018-12-05

文章信息/Info

Title:
Cloning and prokaryotic expression of MYB transcription factor PcMYB2 gene from Polygonum cuspidatum
作者:
李晓筱;  徐俊雄;  刘婵;  王岩岩;  伍翔;  覃建兵;  柳忠玉
长江大学生命科学学院，湖北荆州 434025
Author(s):
Li Xiaoxiao; et al
关键词:
虎杖; MYB转录因子; PcMYB2; 原核表达; 苯丙烷代谢; 原花青素
Keywords:
-
分类号:
S567.23+9.01
DOI:
-
文献标志码:
A
摘要:
为了探究MYB(髓细胞组织增生病毒癌基因同源物)转录因子在虎杖苯丙烷代谢途径中的作用，以虎杖叶片为材料，通过cDNA末端快速扩增(RACE)技术获得1个MYB转录因子，命名为PcMYB2，GenBank登录号为MG020557。序列分析表明，PcMYB2基因的cDNA序列全长为 938 bp，开放阅读框(ORF)为738 bp，编码245个氨基酸，推测蛋白质分子质量为27.917 ku。PcMYB2编码的氨基酸序列具有R2R3-MYB类转录因子的共同特征，其N端具有R2、R3这2个MYB结构域，并且R3结构域中包含1个能与bHLH转录因子相互作用的［D/E］Lx2［R/K］x3Lx6Lx3R基序。进化树分析表明，PcMYB2蛋白与苜蓿中参与原花青素调控的MtPAR聚为1组。将该基因的ORF片段连接到原核表达载体pET20b中，构建融合表达载体pET20b-PcMYB2，转化到大肠杆菌(Escherichia coli) Rosetta(DE3)中进行表达。SDS-PAGE(十二烷基硫酸钠-聚丙烯酰胺凝胶电泳)检测显示表达蛋白与预期大小一致，表明成功克隆PcMYB2基因的cDNA全长序列，构建的原核表达载体pET20b-PcMYB2可用于该基因的功能研究。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-03-25
基金项目：国家自然科学基金(编号：81803670)；国家级大学生创新项目(编号：201810489013)；长江大学校级大学生创新训练计划(编号：2017073)。
作者简介：李晓筱(1998—)，女，湖北仙桃人，主要从事药用植物次生代谢研究。E-mail：xufzy2016@126.com。
通信作者：柳忠玉，博士，讲师，主要从事中药生物技术研究。E-mail：zyliu2004@126.com。
更新日期/Last Update: 2018-12-05