[1]吕孙建,袁雪梅,刘莉,等.宽谱噬菌体NTHP01的生理特征及噬菌作用[J].江苏农业科学,2019,47(03):132-135.
　Lü Sunjian,et al.Biological characterization and host-range identification of broad-spectrum bacteriophage NTHP01[J].Jiangsu Agricultural Sciences,2019,47(03):132-135.
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宽谱噬菌体NTHP01的生理特征及噬菌作用()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第03期
页码:
132-135
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-02-02

文章信息/Info

Title:
Biological characterization and host-range identification of broad-spectrum bacteriophage NTHP01
作者:
吕孙建;  袁雪梅;  刘莉;  卢淑娟;  杭小英;  施伟达
农业部淡水渔业健康养殖重点实验室/浙江省鱼类健康与营养重点实验室/浙江省淡水水产研究所，浙江湖州 313001
Author(s):
Lü Sunjian; et al
关键词:
噬菌体; 生理特征; 抑菌作用; 宿主范围
Keywords:
-
分类号:
S947.1+2
DOI:
-
文献标志码:
A
摘要:
针对1株从中华鳖养殖塘中分离得到的宽谱噬菌体NTHP01(保藏号：CGMCC No. 9623)，对其感染复数、潜伏期和裂解期、热稳定性、pH稳定性等理化特性展开研究，探讨了氯化镁促噬菌体感染的最佳浓度，并考察了该噬菌体的噬菌谱。结果显示，噬菌体NTHP01的最佳感染复数为0.01，潜伏期和裂解期分别为150、60 min，最适温度为 30 ℃，最适pH值为7，氯化镁终浓度为4 mmol/L时对噬菌体的感染促进作用最佳。此外，结果显示噬菌体NTHP01宿主范围宽，能够有效抑制来自不同水产养殖品种的细菌，包括嗜水气单胞菌、脑膜脓毒性黄杆菌、迟缓爱德华氏菌、副溶血性弧菌、维氏气单胞菌、腐败希瓦菌、温和气单胞菌等。研究结果为噬菌体NTHP01的规模化制备及今后在水产养殖细菌性病害防治中的应用奠定了良好基础。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2018-09-19
基金项目：浙江省科技计划项目(编号：2018F10013)。
作者简介：吕孙建(1988—)，男，浙江温州人，博士，助理研究员，主要从事水产动物病害防治。E-mail：lvsunjian@163.com。
通信作者：刘莉，博士，研究员，主要从事水产动物病害防治。E-mail：liuli6655@hotmail.com。
更新日期/Last Update: 2019-02-05