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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第47卷

期数:

2019年第10期

页码:

69-74

栏目:

生物技术

出版日期:

2019-06-12

文章信息/Info

Title:

Identification and expression analysis of CaNramp gene family in pepper

作者:

胡灿¹;  刘峰²;  王运生¹

1.湖南农业大学，湖南长沙 410128； 2.湖南省农业科学院蔬菜研究所，湖南长沙 410128

Author(s):

Hu Can; et al

关键词:

辣椒; NRAMP基因家族; 生物信息学; 基因表达

Keywords:

-

分类号:

S641.301

DOI:

-

文献标志码:

A

摘要:

为了初步研究辣椒(Capsicum annuum L.)NRAMP家族基因在重金属镉胁迫下的表达情况，通过生物信息学方法从辣椒基因组中鉴定出NRAMP基因，并对这些基因的序列结构、系统进化树、表达谱等进行系统分析。结果表明，辣椒中含有5个具有典型NRAMP结构域的基因，根据其在染色体上的位置，分别将其命名为CaNRAMP1~CaNRAMP5，根据系统进化分析，将辣椒的NRAMP基因分为2类，Ⅰ类包含2个基因(CaNRAMP1、CaNRAMP3)，分别含有10、12个内含子，Ⅱ类包含3个基因(CaNRAMP2、CaNRAMP4、CaNRAMP5)，均含有3个内含子。基于基因组织表达模式的分析结果表明，CaNRAMP1在不同组织中均未检测到其表达；CaNRAMP5的表达集中在花器官，其余CaNRAMP基因均有不同的表达模式；通过逆转录定量PCR(qRT-PCR)分析发现，辣椒苗期中的叶片经过镉处理后，CaNRAMP3基因呈明显上调的趋势，参与镉胁迫反应的正向调控，其他基因则不参与镉胁迫反应的调控。研究结果为进一步分析辣椒NRAMP基因家族的功能奠定了基础。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2018-03-16
基金项目：国家自然科学基金(编号：31470105)；南方蔬菜优质高效生产关键技术研究与示范项目(编号：2014BAD05B04)。
作者简介：胡灿(1991—)，男，湖南岳阳人，硕士研究生，主要从事微生物与植物分子互作研究。E-mail：912514197@qq.com。
通信作者：王运生，博士，教授，主要从事生物信息学研究。E-mail：54757519@qq.com。

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更新日期/Last Update: 2019-05-20