[1]王琳,魏启舜,周影,等.细菌对废弃羽毛的降解及在可持续农业中应用[J].江苏农业科学,2020,48(21):40-45.
　Wang Lin,et al.Degradation of waste feathers by bacteria and its application in sustainable agriculture[J].Jiangsu Agricultural Sciences,2020,48(21):40-45.
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细菌对废弃羽毛的降解及在可持续农业中应用()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年21期
页码:
40-45
栏目:
专论与综述
出版日期:
2020-11-05

文章信息/Info

Title:
Degradation of waste feathers by bacteria and its application in sustainable agriculture
作者:
王琳;  魏启舜;  周影;  张培;  郭成宝
江苏丘陵地区南京农业科学研究所，江苏南京 210046
Author(s):
Wang Lin; et al
关键词:
生物肥料; 羽毛角蛋白; 角蛋白降解菌; 有机肥料
Keywords:
-
分类号:
X172；X713
DOI:
-
文献标志码:
A
摘要:
羽毛质量约占鸡总质量的5%~7%，已成为家禽屠宰的主要废弃物。羽毛中90%以上的成分为角蛋白，可作为多肽、氨基酸类有机肥来源。利用传统理化方法降解羽毛能耗大，且可造成氨基酸结构破坏。相比之下，利用角蛋白降解细菌降解羽毛高效节能、反应条件温和，具有广阔的市场前景。本文着重介绍角蛋白降解细菌种类、产酶特点以及角蛋白降解机制，角蛋白降解产物及降解细菌对植物具有抗病促生作用，废弃羽毛的生物降解及肥料化利用可促进有机农业发展、改善农业生态系统和提高土壤生物活性。
Abstract:
-

参考文献/References:

［1］Mckittrick J，Chen P Y，Bodde S G，et al. The structure，functions，and mechanical properties of keratin［J］. JOM，2012，64(4)：449-468.
［2］Meyers M A，Chen P Y，Lin A Y，et al. Biological materials：structure and mechanical properties［J］. Progress in Materials Science，2008，53(1)：1-206.
［3］贾如琰，何玉凤，王荣民，等. 角蛋白的分子构成、提取及应用［J］. 化学通报，2008(1)：1-6.
［4］刘柏宏，张娟，堵国成，等. 地衣芽孢杆菌来源角蛋白酶N端对活力及其热稳定性的影响［J］. 微生物学通报，2014，41(8)：1491-1497.
［5］Rajput R，Tiwary E，Sharma R，et al. Swapping of pro-sequences between keratinases of Bacillus licheniformis and Bacillus pumilus：altered substrate specificity and thermostability［J］. Enzyme and Microbial Technology，2012，51(3)：131-138.
［6］Abdel-Naby M A，El-Refai H A，Mohammad H I. Structural characterization，catalytic，kinetic and thermodynamic properties of keratinase from Bacillus pumilus FH9［J］. International Journal of Biological Macromolecules，2017，105(1)：973-980.
［7］E Silva L A D，Macedo A J，Termignoni C . Production of keratinase by Bacillus subtilis S14［J］. Annals of Microbiology，2014，64(4)：1-9.
［8］Zhen F，Juan Z，Liu B H，et al. Cloning，heterologous expression and characterization of two keratinases from Stenotrophomonas maltophilia BBE11-1［J］. Process Biochemistry，2014，49(4)：647-654.
［9］Habbeche A，Saoudi B，Jaouadi B，et al. Purification and biochemical characterization of a detergent-stable keratinase from a newly thermophilic actinomycete Actinomadura keratinilytica strain Cpt29 isolated from poultry compost［J］. Journal of Bioscience and Bioengineering，2014，117(4)：413-421.
［10］Xie F，Chao Y，Yang X，et al. Purification and characterization of four keratinases produced by Streptomyces sp. strain 16 in native human foot skin medium［J］. Bioresource Technology，2010，101(1)：344-350.
［11］Lin X，Lee C G，Casale E S，et al. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain［J］. Applied and Environmental Microbiology，1992，58(10)：3271-3275.
［12］Brandelli A，Daroit D J，Riffel A. Biochemical features of microbial keratinases and their production and applications［J］. Applied Microbiology and Biotechnology，2010，85(6)：1735-1750.
［13］Riffel A，Brandelli A，Bellato C D，et al. Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. kr6［J］. Journal of Biotechnology，2007，128(3)：693-703.
［14］Azeredo L I，Lima M B，Coelho R R，et al. Thermophilic protease production by Streptomyces sp. 594 in submerged and solid-state fermentations using feather meal［J］. Journal of Applied Microbiology，2006，100(4)：641-647.
［15］Wang S L，Wan-Ting H，Tzu-Wen L，et al. Purification and characterization of three novel keratinolytic metalloproteases produced by Chryseobacterium indologenes TKU014 in a shrimp shell powder medium［J］. Bioresource Technology，2008，99(13)：5679-5686.
［16］Silveira S T，Gemelli S，Segalin J，et al. Immobilization of keratinolytic metalloprotease from Chryseobacterium sp. strain kr6 on glutaraldehyde-activated chitosan［J］. Journal of Microbiology and Biotechnology，2012，22(6)：818-825.
［17］Balaji S，Kumar M S，Karthikeyan R，et al. Purification and characterization of an extracellular keratinase from a hornmeal-degrading Bacillus subtilis MTCC (9102)［J］. World Journal of Microbiology and Biotechnology，2008，24(11)：2741-2745.
［18］Thys R S，Brandelli A. Purification and properties of a keratinolytic metalloprotease from Microbacterium sp.［J］. Journal of Applied Microbiology，2006，101(6)：1259-1268.
［19］Lin H H，Yin L J，Jiang S T. Expression and purification of Pseudomonas aeruginosa keratinase in Bacillus subtilis DB104 expression system［J］. Journal of Agricultural and Food Chemistry，2009，57(17)：7779-7784.
［20］Shinji M，Ichikawa M，Oka T，et al. Molecular characterization of a keratinolytic enzyme from an alkaliphilic Nocardiopsis sp. TOA-1［J］. Enzyme and Microbial Technology，2004，34(5)：482-489.
［21］Park G T，Son H J. Keratinolytic activity of Bacillus megaterium F7-1，a feather-degrading mesophilic bacterium［J］. Microbiological Research，2009，164(4)：478-485.
［22］Kim J M，Lim W J，Suh H J. Feather-degrading Bacillus species from poultry waste［J］. Process Biochemistry，2001，37(3)：287-291.
［23］李光磊，张娟，方真，等. 嗜麦芽寡养单胞菌角蛋白酶基因在毕赤酵母中的表达［J］. 生物技术通报，2016，32(8)：152-160.
［24］Bressollier P，Letourneau F，Urdaci M，et al. Purification and characterization of a keratinolytic serine proteinase from Streptomyces albidoflavus［J］. Applied and Environmental Microbiology，1999，65(6)：2570-2576.
［25］Chitte R R，Nalawade V K，Dey S. Keratinolytic activity from the broth of a feather-degrading thermophilic Streptomyces thermoviolaceus strain SD8［J］. Letters in Applied Microbiology，1999，28(2)：131-136.
［26］Production K T. Characterization and application of keratinase from Streptomyces gulbargensis［J］. Bioresource Technology，2009，100(5)：1868-1871.
［27］Friedrich A B，Antranikian G. Keratin degradation by Fervidobacterium pennavorans，a novel thermophilic anaerobic species of the order thermotogales［J］. Applied and Environmental Microbiology，1996，62(8)：2875-2882.
［28］Nam G W，Lee D W，Lee H S，et al. Native-feather degradation by Fervidobacterium islandicum AW-1，a newly isolated keratinase-producing thermophilic anaerobe［J］. Archives of Microbiology，2002，178(6)：538-547.
［29］徐国恒. 二硫键与蛋白质的结构［J］. 生物学通报，2010，45(5)：5-7.
［30］仇晓燕，崔勐，刘志强，等. 蛋白质中二硫键的定位及其质谱分析［J］. 化学进展，2008，20(6)：975-983.
［31］Grumbt M，Monod M，Yamada T，et al. Keratin degradation by dermatophytes relies on cysteine dioxygenase and a sulfite efflux pump［J］. The Journal of Investigative Dermatology，2013，133(6)：1550-1555.
［32］朱晓飞，张玲，赵平芝，等. 链霉菌B221的角蛋白降解机制初探［J］. 中国农学通报，2007，23(6)：18-22.
［33］Yamamura S，Morita Y，Hasan Q，et al. Keratin degradation：a cooperative action of two enzymes from Stenotrophomonas sp.［J］. Biochemical and Biophysical Research Communications，2002，294(5)：1138-1143.
［34］Rahayu S，Syah D，Suhartono M T. Degradation of keratin by keratinase and disulfide reductase from Bacillus sp. MTS of Indonesian origin［J］. Biocatalysis and Agricultural Biotechnology，2012，1(2)：152-158.
［35］Prakash P J S，Characterization of extreme alkaline. Thermostable keratinase，and keratin disulfide reductase produced by Bacillus halodurans PPKS-2［J］. Applied Microbiology and Biotechnology，2010，87(2)：625-633.
［36］Huang Y H，Busk P K，Lange L. Production and characterization of keratinolytic proteases produced by Onygena corvina［J］. Fungal Genomics & Biology，2015，05(1)：119.
［37］魏启舜，赵荷娟，周影，等. 施用羽毛生物降解液对白菜生长和基质养分的影响［J］. 江苏农业科学，2019，47(20)：156-159.
［38］魏启舜，赵荷娟，郭成宝，等. 羽毛生物降解氨基酸肥对草莓生长和果实品质的影响［J］. 中国农学通报，2019，35(32)：46-52.
［39］Nagarajan S，Eswaran P，Masilamani R P，et al. Chicken feather compost to promote the plant growth activity by using keratinolytic bacteria［J］. Waste and Biomass Valorization，2018，9(4)：531-538.
［40］Jin-Ha J，Lee O M，Young-Dong J，et al. Production of keratinolytic enzyme by a newly isolated feather-degrading Stenotrophomonas maltophilia that produces plant growth-promoting activity［J］. Process Biochemistry，2010，45(10)：1738-1745.
［41］Kshetri P，Roy S S，Sharma S K，et al. Transforming chicken feather waste into feather protein hydrolysate using a newly isolated multifaceted keratinolytic bacterium Chryseobacterium sediminis RCM-SSR-7［J］. Waste and Biomass Valorization，2019，10(1)：1-11.
［42］Bhange K，Chaturvedi V，Bhatt R. Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1［J］. Bioresources and Bioprocessing，2016，3(1)：13.
［43］Khan M S，Zaidi A，Wani P A. Role of phosphate-solubilizing microorganisms in sustainable agriculture-A review［J］. Agronomy for Sustainable Development，2007，27(1)：29-43.
［44］张艾明.磷在农田土壤中的迁移转化规律及其对农业环境的影响［C］//组学大数据整合生物信息学研讨会论文集，2017.
［45］Rodriguez H，Gonzalez T，Goire I，et al. Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp［J］. Die Naturwissenschaften，2004，91(11)：552-555.
［46］Kaur G，Reddy M S. Influence of P-solubilizing bacteria on crop yield and soil fertility at multilocational sites［J］. European Journal of Soil Biology，2014，61：35-40.
［47］Sudhir K R，Mukherjee A K. Optimization for production of liquid nitrogen fertilizer from the degradation of chicken feather by iron-oxide (Fe3O4) magnetic nanoparticles coupled β-keratinase［J］. Biocatalysis and Agricultural Biotechnology，2015，4(4)：632-644.
［48］Teale W D，Paponov I A，Palme K. Auxin in action：signalling，transport and the control of plant growth and development［J］. Nature Reviews Molecular Cell Biology，2006，7(11)：847-859.
［49］Paul T，Halder S K，Das A，et al. Exploitation of chicken feather waste as a plant growth promoting agent using keratinase producing novel isolate Paenibacillus woosongensis TKB2［J］. Biocatalysis and Agricultural Biotechnology，2013，2(1)：50-57.
［50］Asik B B，Turan M A，Celik H，et al. Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv. Salihli) under conditions of salinity［J］. Asian Journal of Crop Science，2009，1(2)：87-95.
［51］Rai S K，Mukherjee A K. Optimization of production of an oxidant and detergent-stable alkaline β-keratinase from Brevibacillus sp. strain AS-S10-II：application of enzyme in laundry detergent formulations and in leather industry［J］. Biochemical Engineering Journal，2011，54(1)：47-56.
［52］Verma A，Singh H，Anwar S，et al. Microbial keratinases：industrial enzymes with waste management potential［J］. Critical Reviews in Biotechnology，2017，37(4)：476-491.
［53］Gupta R，Sharma R，Beg Q K. Revisiting microbial keratinases：next generation proteases for sustainable biotechnology［J］. Critical Reviews in Biotechnology，2013，33(2)：216-228.
［54］王琳，钱玉婷，周影，等. 高温放线菌YT06降解羽毛的研究［J］. 生物加工过程，2018，16(4)：49-56.
［55］Wang L，Qian Y T，Cao Y，et al. Production and characterization of keratinolytic proteases by a chicken feather-degrading thermophilic strain，Thermoactinomyces sp. YT06［J］. Journal of Microbiology and Biotechnology，2017，27(12)：2190-2198.
［56］Adriano B，Sala L，Kalil S J. Microbial enzymes for bioconversion of poultry waste into added-value products［J］. Food Research International，2015，73：3-12.
［57］孙旭，刘臣炜，张龙江，等. 农业废弃物制备生物有机肥及其在小白菜栽培上的应用［J］. 江苏农业学报，2017，33(6)：1333-1341.
［58］Lmpdi K. Development of an environmentally friendly biofertilizer with keratin degrading and antibiotic producing actinomycetes［J］. Actinomycetologica，2004，18(2)：34-42.
［59］周莲，谢小林，顾振红，等. 解淀粉芽孢杆菌3-2发酵羽毛产氨基酸［J］. 微生物学通报，2017，44(11)：2511-2521.

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备注/Memo

备注/Memo:
收稿日期：2020-02-21
基金项目：江苏省科技项目现代农业-重点及面上项目 (编号：BE2019301)；国家自然科学基金青年科学基金(编号：31700099)。
作者简介：王琳(1980—)，女，江苏泰州人，博士，副研究员，主要从事农业废弃物资源化利用研究。E-mail：wanglin0421nj@163.com。
通信作者：郭成宝，硕士，研究员，主要从事农业废弃物资源化利用研究。E-mail：gchengbao@163.com。
更新日期/Last Update: 2020-11-05