[1]姚鹏强,袁轶,程世平.张良姜同源四倍体诱导[J].江苏农业科学,2021,49(2):104-107.
 Yao Pengqiang,et al.Induction of autotetraploid of Zhangliang giner[J].Jiangsu Agricultural Sciences,2021,49(2):104-107.
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张良姜同源四倍体诱导()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第2期
页码:
104-107
栏目:
园艺与林学
出版日期:
2021-01-20

文章信息/Info

Title:
Induction of autotetraploid of Zhangliang giner
作者:
姚鹏强 袁轶 程世平
平顶山学院化学与环境工程学院,河南平顶山 467000
Author(s):
Yao Pengqianget al
关键词:
张良姜离体培养染色体加倍同源四倍体
Keywords:
-
分类号:
S632.503
DOI:
-
文献标志码:
A
摘要:
为解决张良姜繁殖系数低、容易感染病菌的问题,进一步加快张良姜育种进程,以河南省鲁山县张良镇种姜为材料,首先建立张良姜茎尖离体培养再生体系,进一步利用秋水仙碱对其体细胞染色体进行诱导加倍。结果表明,张良姜茎尖不定芽诱导的最适培养基为MS+6-BA 2.0 mg/L+NAA 01 mg/L,不定芽诱导率达83.33%。不定芽生根的最适培养基为1/2 MS+NAA 0.5 mg/L,生根率可达86.67%。张良姜同源四倍体诱导最佳处理组合为100 mg/L秋水仙碱浸泡茎尖72 h再接种培养,四倍体最高诱导率为8.89%。对照组张良姜为二倍体(2n=2x=22),诱导获得的四倍体张良姜染色体为2n=4x=44。共获得7个四倍体植株,继代培养3次后倍性保持稳定,可为张良姜良种选育提供良好的原始材料。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2020-05-19
基金项目:平顶山学院高层次人才科研启动项目(编号:BXY-BSQD-2018031、PXY-BSQD2016009);河南省科技攻关计划(编号:182102110132、172102110111)。
作者简介:姚鹏强(1986—),男,云南曲靖人,博士,讲师,主要研究方向为植物多倍体遗传育种。E-mail:yaopengqiang1988@163.com。
更新日期/Last Update: 2021-01-20