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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第50卷

期数:

2022年第1期

页码:

34-39

栏目:

生物技术

出版日期:

2022-01-05

文章信息/Info

Title:

Development of InDel markers for arbitrary region of rice genome

作者:

孙玥; 杨秀荣²; 郭彦丽¹; 李军玲¹; 李月娇²; 孙淑琴; 路信; 刘燕清; 佟卉<; 孙林静; 刘静妍; 张融雪; 王晓静; 苏京平; 王胜军; 赵习朴; 闫双勇

1.天津市农业科学院农作物研究所/天津市农作物遗传育种重点实验室，天津 300384；2.天津市农业科学院植物保护研究所，天津 300384

Author(s):

Sun Yue; et al

关键词:

水稻; 分子标记开发; 高通量测序; QTL定位(QTL-seq); InDel标记; 多态频率

Keywords:

-

分类号:

S511.01

DOI:

-

文献标志码:

A

摘要:

基于极端表型单株高通量测序的定位方法QTL-seq已成为植物QTL分析的一种主要方法。继QTL-seq分析后，对目标QTL定位区间进行分子标记开发时，由于目标区间大，所含变异位点多，特定区间的分子标记开发仍是一件较繁琐的事。为提高特定区间分子标记开发的效率，本研究建立了基于高通量测序数据基础的特定脚本程序，可简单快速地完成水稻基因组任意区间的InDel标记开发。本研究开发了来自7个不同作图群体和染色体区间的有8 bp以上片段长度差异的InDel标记708个，平均每17.6 kb有1个InDel标记；对95个标记进行试验验证，总体多态频率为63%。本研究建立的方法在水稻及其他已测序的植物重要农艺性状控制QTL的分子标记辅助育种、精细定位和图位克隆中有重要应用价值。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2021-04-08
基金项目：国家重点研发计划(编号：2017YFD0100506)；天津市支撑计划(编号：18YFZCNC01210)；天津市自然科学基金(编号：19JCYBJC29500)；天津市水稻现代农业产业技术体系创新团队项目(编号：ITTRRS2018015)。
作者简介：孙玥(1982—)，女，吉林长春人，博士，副研究员，主要从事作物栽培生理研究，E-mail：13102091103@163.com；共同第一作者：杨秀荣(1972—)，女，天津武清人，硕士，副

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更新日期/Last Update: 2022-01-05