[1]吴小军,汪汉成,刘亭亭,等.嘧菌酯处理后烟叶叶际微生物在不同时期的响应[J].江苏农业科学,2023,51(24):123-132.
 Wu Xiaojun,et al.Response of phyllosphere microbial in tobacco leaves after azoxystrobin treatment at different periods[J].Jiangsu Agricultural Sciences,2023,51(24):123-132.
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嘧菌酯处理后烟叶叶际微生物在不同时期的响应()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第24期
页码:
123-132
栏目:
植物保护
出版日期:
2023-12-20

文章信息/Info

Title:
Response of phyllosphere microbial in tobacco leaves after azoxystrobin treatment at different periods
作者:
吴小军12汪汉成2刘亭亭3蔡刘体2孟建玉2彭丽娟1
1.贵州大学烟草学院,贵州贵阳 550025; 2.贵州省烟草科学研究院,贵州贵阳 550081;3.长江大学农学院,湖北荆州 434025
Author(s):
Wu Xiaojunet al
关键词:
嘧菌酯烟草赤星病群落结构代谢功能微生物多样性
Keywords:
-
分类号:
S182;S435.72
DOI:
-
文献标志码:
A
摘要:
为研究烟叶不同部位叶际微生物群落在嘧菌酯处理后不同时期的变化规律,采用高通量测序和Biolog代谢表型技术分别测定了烟叶不同部位叶际微生物在嘧菌酯处理前后不同时期的群落结构、多样性及代谢功能。结果表明,相较于处理前,250 g/L嘧菌酯SC(0.15 kg a.i./hm2)处理后0~15 d内,叶际真菌群落中,感病与健康烟叶叶际优势真菌属种类均增加。感病烟叶叶际链格孢属、壳多孢菌属和茎点霉属相对丰度均先增加后降低;枝孢霉属、亚隔孢壳属和Symmetrospora则先降低后增加。健康烟叶叶际链格孢属、枝孢霉属、亚隔孢壳属和Symmetrospora相对丰度均先降低后增加;壳多孢菌属、假丝酵母属、浪梗霉属和蜡壳菌属则先增加,并在处理后5 d时成为新的优势菌属。在细菌群落中,感病与健康烟叶叶际泛菌属、马赛菌属和鞘脂单胞菌属相对丰度均持续降低,感病烟叶叶际的假单胞菌属、乳杆菌属、肠杆菌属和甲基杆菌属丰度先增加后降低。感病烟叶叶际真菌群落多样性和丰富度持续增加,细菌群落多样性先增加后降低,丰富度先降低后增加;健康烟叶叶际真菌群落多样性和丰富度先增加后降低,细菌群落多样性先降低后增加,丰富度先增加后降低。嘧菌酯处理后5 d时对感病烟叶叶际微生物的碳源代谢抑制作用较健康烟叶弱,但10 d时则均表现出较强抑制作用。研究结果揭示了嘧菌酯施用后15 d内烟叶叶际真菌和细菌群落结构、多样性及代谢功能的时序变化规律,为了解嘧菌酯防控烟草赤星病的微观作用机制提供参考依据。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2023-03-17
项目基金:中国烟草总公司科技项目[编号:110202101048(LS-08)、110202001035(LS-04)];国家自然科学基金(编号:32160522、31960550);贵州省“百层次”创新型人才项目(编号:黔科合平台人才-GCC[2022]028-1);贵州省科技基金(编号:黔科合基础-ZK[2021]重点036)。
作者简介:吴小军(1997—),男,贵州思南人,硕士研究生,从事烟草植保研究。E-mail:2939400539@qq.com。
通信作者:汪汉成,博士,研究员,从事植物保护研究,E-mail:xiaobaiyang126@hotmail.com;彭丽娟,博士,教授,从事植物病理研究,E-mail:296430006@qq.com。
更新日期/Last Update: 2023-12-20