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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第52卷

期数:

2024年第7期

页码:

17-24

栏目:

专论与综述

出版日期:

2024-04-05

文章信息/Info

Title:

Application of loop-mediated isothermal amplification(LAMP) in agricultural disease detection：a review

作者:

王一波; 孙泓希; 史普想; 孙继军; 韩宁; 任亮; 张丽丽; 王海新

辽宁省沙地治理与利用研究所，辽宁阜新 123000

Author(s):

Wang Yibo; et al

关键词:

环介导等温扩增技术; 农业病害; 检测; 应用

Keywords:

-

分类号:

S431.9；S432.4

DOI:

-

文献标志码:

A

摘要:

环介导等温扩增技术(loop-mediated isothermal amplification，LAMP)是一种新型核酸等温扩增技术，其原理是当温度为60~65 ℃时，DNA处于动态平衡状态，DNA双链打开，此时依赖Bst DNA聚合酶的链置换活性，使DNA可以在60~65 ℃的恒温下进行合成。其扩增引物是基于靶基因的6个特异性区域设计的，扩增阶段需要引物与样品DNA完全结合，才可以进行扩增，所以LAMP技术具有高特异性与高灵敏性。其反应温度恒定，不需要额外的控温设备，理想条件下仅需保温杯，即可进行DNA扩增。在田间检测时，只需提取样品总DNA，即可于田间进行病害检测，缩短了检测流程及时间，使病害检测更高效更便捷。在反应前或反应后加入特定染色剂，即可通过染色剂的变色情况进行反应结果的判定，使反应结果可视化。目前该技术已凭借其特异性高、灵敏性高、所用仪器简单(恒温水浴锅或保温杯即可)、检测效率高和检测结果可视化等优点，广泛应用于临床病原微生物检测、食品微生物检测、环境微生物检测、植物或微生物基因鉴定等领域。在农业病害的病原微生物(真菌、细菌、病毒、线虫)快速检测和诊断领域，已有很多研究针对各种病害建立了相应的LAMP检测技术。本文简要综述了LAMP技术原理、反应结果判定、优缺点和该技术目前在农业常见病原物检测中的应用，并对LAMP技术的应用前景进行了进一步的展望。

Abstract:

-

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相似文献/References:

[1]张涛涛,王兰,龚频,等.环介导等温扩增技术快速检测金黄色葡萄球菌[J].江苏农业科学,2014,42(02):238.
　Zhang Taotao,et al.Rapid detection of Staphylococcus aureus by loop-mediated isothermal amplification technology[J].Jiangsu Agricultural Sciences,2014,42(7):238.
[2]魏桢元.环介导等温扩增技术在食源性沙门氏菌检测中的应用研究进展[J].江苏农业科学,2020,48(03):80.
　Wei Zhenyuan.Research progress on application of loop-mediated isothermal amplification(LAMP) for detection of salmonella in food[J].Jiangsu Agricultural Sciences,2020,48(7):80.
[3]王晓莉,李哲,杨红福,等.应用环介导等温扩增技术检测江苏省水稻苗期与成株期的恶苗病病菌[J].江苏农业科学,2020,48(20):110.
　Wang Xiaoli,et al.Detection of Fusarium spp. causing rice bakanae disease at seedling and adult stages based on loop-mediated isothermal amplification technology (LAMP) assays[J].Jiangsu Agricultural Sciences,2020,48(7):110.

备注/Memo

备注/Memo:

收稿日期：2023-06-06
基金项目：辽宁省科技项目(编号：2021JH/10400034、2022JH2/101300164) 。
作者简介：王一波(1994—)，男，辽宁沈阳人，硕士，研究实习员，从事花生栽培及病害防控研究研究。E-mail：465910393@qq.com。
通信作者：王海新，研究员，从事花生栽培研究和推广工作。E-mail：wanghaixin99@163.com。

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更新日期/Last Update: 2024-04-05