[1]杜灿灿,曾生元,景德道,等.基于BSA-seq的一个苗期黄化转绿突变体基因定位[J].江苏农业科学,2024,52(12):53-60.
 Du Cancan,et al.Gene mapping of a green-reversible yellowish mutant at seedling stage based on BSA-seq[J].Jiangsu Agricultural Sciences,2024,52(12):53-60.
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基于BSA-seq的一个苗期黄化转绿突变体基因定位()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第12期
页码:
53-60
栏目:
生物技术
出版日期:
2024-06-20

文章信息/Info

Title:
Gene mapping of a green-reversible yellowish mutant at seedling stage based on BSA-seq
作者:
杜灿灿曾生元景德道胡庆峰李闯钱华飞林添资余波孙立亭周义文杨军韩华新龚红兵
江苏丘陵地区镇江农业科学研究所,江苏句容 212400
Author(s):
Du Cancanet al
关键词:
苗期黄化转绿突变体BSA-seqΔSNP-index算法ED算法
Keywords:
-
分类号:
S511.01
DOI:
-
文献标志码:
A
摘要:
叶片是植物进行光合作用的重要场所,叶色突变往往制约水稻具有充足的源,研究叶色突变体对选育高光效品种以及进一步解析叶色调控的分子机制均具有重要意义。以育种中间材料苗期叶片黄化转绿突变体gry为对象,对其进行主要农艺性状分析,并对其突变性状进行遗传分析、基因定位。结果显示,该突变体3叶期前植株呈淡黄色至白色,4叶期变绿,除成熟期株高显著高于野生型以外,其他主要农艺性状均无显著差异。通过遗传分析确定该突变体的黄化转绿表型受隐性单基因控制。利用gry突变体与野生型的F2群体构建混池,通过BSA-seq的ΔSNP-index和ED 2种算法进行基因定位,结果显示gry基因被定位在2223~26.77 Mb区间内。在该区间内开发KASP标记对62个F2黄化转绿单株进行验证与连锁分析,验证gry基因在21.18~25.30 Mb区间内。基于定位区间,结合基因注释数据库与水稻基因变异数据库推测候选基因为LOC_Os03g40020,测序表明该基因在第1 768位发生单碱基T缺失,导致编码的氨基酸大量改变而产生功能变化。
Abstract:
-

参考文献/References:

[1]邵奎添. 水稻库源基因NAL1的生理功能研究[D]. 沈阳:沈阳农业大学,2022.
[2]贺治洲,尹明,谢振宇,等. 水稻转绿型叶色突变体研究进展[J]. 热带农业科学,2014,34(8):30-36,42.
[3]杨颜榕,黄纤纤,赵亚男,等. 水稻叶色基因克隆与分子机制研究进展[J]. 植物遗传资源学报,2020,21(4):794-803.
[4]王丰. 水稻类病斑突变体lrd25的基因定位及特征特性分析[D]. 沈阳:沈阳农业大学,2022.
[5]廉院训,韦子芸,张强,等. 水稻斑马叶突变体zl7的鉴定与基因的精细定位[J]. 中国水稻科学,2023,37(2):113-124.
[6]刘亚萍. 水稻深绿穗基因DGP1的克隆与功能研究[D]. 金华:浙江师范大学,2017.
[7]李洋洋. 微型紫叶观赏水稻新种质“紫薇” 的创制[D]. 重庆:西南大学,2020.
[8]胡婷婷,何弯弯,王友霜,等. 水稻白化转绿突变体al14的表型分析及基因定位[J]. 江苏农业学报,2021,37(6):1361-1369.
[9]Yue R Q,Wang X F,Chen J Y,et al. A rice stromal processing peptidase regulates chloroplast and root development[J]. Plant and Cell Physiology,2010,51(3):475-485.
[10]赵冬生. 水稻幼苗白化致死基因AL1和粒形基因GS9的克隆与功能分析[D]. 扬州:扬州大学,2016.
[11]Li W,Wu C,Hu G C,et al. Characterization and fine mapping of a novel rice narrow leaf mutant nal9[J]. Journal of Integrative Plant Biology,2013,55(11):1016-1025.
[12]李家飞. 水稻白化突变体albl和alblhc2的光合特性和基因克隆[D]. 上海:上海师范大学,2011.
[13]李春梅. 两个水稻黄绿叶突变基因的图位克隆及功能分析[D]. 雅安:四川农业大学,2019.
[14]Yamatani H,Kohzuma K,Nakano M,et al. Impairment of Lhca4,a subunit of LHCI,causes high accumulation of chlorophyll and the stay-green phenotype in rice[J]. Journal of Experimental Botany,2018,69(5):1027-1035.
[15]Yang Y L,Xu J,Huang L C,et al. PGL,encoding chlorophyllide a oxygenase 1,impacts leaf senescence and indirectly affects grain yield and quality in rice[J]. Journal of Experimental Botany,2016,67(5):1297-1310.
[16]Deng X J,Zhang H Q,Wang Y,et al. Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L.ssp.indica)[J]. PLoS One,2014,9(6):e99564.
[17]Wu Z M,Zhang X,He B,et al. A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis[J]. Plant Physiology,2007,145(1):29-40.
[18]Shin D,Lee S,Kim T H,et al. Natural variations at the Stay-Green gene promoter control lifespan and yield in rice cultivars[J]. Nature Communications,2020,11:2819.
[19]Pusnik M,Small I,Read L K,et al. Pentatricopeptide repeat proteins in Trypanosoma brucei function in mitochondrial ribosomes[J]. Molecular and Cellular Biology,2007,27(19):6876-6888.
[20]李景芳,王宝祥,刘艳,等. PPR蛋白在水稻生长发育中的功能研究进展[J]. 植物遗传资源学报,2022,23(2):358-367.
[21]Li S B,Sun Q P,Hu M H,et al. Phylogenetic genomewide comparisons of the pentatricopeptide repeat gene family in indica and japonica rice[J]. Biochemical Genetics,2012,50(11):978-989.
[22]Yu M Z,Wu M M,Ren Y L,et al. Rice FLOURY ENDOSPERM 18 encodes a pentatricopeptide repeat protein required for 5′ processing of mitochondrial nad5 messenger RNA and endosperm development[J]. Journal of Integrative Plant Biology,2021,63(5):834-847.
[23]Zheng S Y,Dong J F,Lu J Q,et al. A cytosolic pentatricopeptide repeat protein is essential for tapetal plastid development by regulating OsGLK1 transcript levels in rice[J]. New Phytologist,2022,234(5):1678-1695.
[24]Su N,Hu M L,Wu D X,et al. Disruption of a rice pentatricopeptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production[J]. Plant Physiology,2012,159(1):227-238.
[25]李辉平,骆昕,侯子强,等. 基于BSA-seq技术挖掘糙皮侧耳抗螨候选基因[J]. 江苏农业学报,2022,38(6):1648-1656.
[26]Liang T M,Chi W C,Huang L K,et al. Bulked segregant analysis coupled with whole-genome sequencing (BSA-seq) mapping identifies a novel pi21 haplotype conferring basal resistance to rice blast disease[J]. International Journal of Molecular Sciences,2020,21(6):2162.
[27]Xin W,Liu H L,Yang L M,et al. BSA-seq and fine linkage mapping for the identification of a novel locus (qPH9) for mature plant height in rice (Oryza sativa)[J]. Rice,2022,15(1):26.
[28]Kaur G,Yadav I S,Bhatia D,et al. BSA-seq identifies a major locus on chromosome 6 for root-knot nematode (Meloidogyne graminicola) resistance from Oryza glaberrima[J]. Frontiers in Genetics,2022,13:871833.
[29]Takagi H,Abe A,Yoshida K,et al. QTL-seq:rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations[J]. The Plant Journal,2013,74(1):174-183.
[30]Li H,Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform[J]. Bioinformatics,2009,25(14):1754-1760.
[31]Abe A,Kosugi S,Yoshida K,et al. Genome sequencing reveals agronomically important loci in rice using MutMap[J]. Nature Biotechnology,2012,30(2):174-178.
[32]Hill J T,Demarest B L,Bisgrove B W,et al. MMAPPR:mutation mapping analysis pipeline for pooled RNA-seq[J]. Genome Research,2013,23(4):687-697.
[33]Zhao H,Li J C,Yang L,et al. An inferred functional impact map of genetic variants in rice[J]. Molecular Plant,2021,14(9):1584-1599.
[34]姚晓云,蓝海军,邓伟,等. 水稻淡白叶突变体的叶绿素含量测定及农艺性状比较分析[J]. 江西农业学报,2020,32(12):12-15.
[35]Chen D G,Zhou X Q,Chen K,et al. Fine-mapping and candidate gene analysis of a major locus controlling leaf thickness in rice (Oryza sativa L.)[J]. Molecular Breeding,2022,42(2):6.
[36]郭微. 利用QTL定位和BSA-seq分析鉴定碱胁迫下水稻产量相关性状的候选基因[D]. 哈尔滨:东北农业大学,2019.

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备注/Memo

备注/Memo:
收稿日期:2023-06-30
基金项目:句容市农业科技支撑计划(编号:ZA32221);镇江市农业科学院青年基金(编号:QNJJ2018002)。
作者简介:杜灿灿(1989—),女,江苏徐州人,硕士,助理研究员,主要从事水稻遗传育种及推广应用研究。E-mail:20162804@jaas.ac.cn。
通信作者:龚红兵,硕士,研究员,主要从事水稻新品种选育及推广应用研究。E-mail:hongbinggong973@sina.com。
更新日期/Last Update: 2024-06-20