|本期目录/Table of Contents|

[1]窦勇,闾怀中,孔令伟,等.ε-聚赖氨酸对苹果采后灰霉病防治效果及机理[J].江苏农业科学,2024,52(23):187-194.
　Dou Yong,et al.Preventive effect and mechanism of ε- polylysine on gray mold of postharvest apple[J].Jiangsu Agricultural Sciences,2024,52(23):187-194.
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ε-聚赖氨酸对苹果采后灰霉病防治效果及机理(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第23期
页码:
187-194
栏目:
贮藏加工与检测分析
出版日期:
2024-12-05

文章信息/Info

Title:
Preventive effect and mechanism of ε- polylysine on gray mold of postharvest apple
作者:
窦勇1; 闾怀中1; 孔令伟2; 董静1; 姚妙爱1
1.江苏财经职业技术学院粮食与食品药品学院，江苏淮安 223003； 2.淮安快鹿牛奶有限公司，江苏淮安 223311
Author(s):
Dou Yong; et al
关键词:
ε-PL; 生理机制; 灰霉病; 苹果; 诱导抗性; 防治效果
Keywords:
-
分类号:
TS255.3
DOI:
-
文献标志码:
A
摘要:
为探寻高效、安全的苹果采后灰霉病的控制方法，研究 ε-聚赖氨酸(ε-poly-L-lysine，简称ε-PL)对苹果采后灰霉病的防治效果及其抗性诱导机制，以无菌水为对照，采用不同浓度的 ε-PL诱导处理苹果24 h后，探究 ε-PL 控制苹果灰霉病的最佳使用浓度。将 ε-PL分别用打孔注入和整果浸泡的方法处理苹果，研究其对苹果抗性物质分泌、抗性酶活性及其编码基因表达的诱导机制，同时考察其对苹果自然腐烂和贮藏品质的影响。结果表明，400 mg/L 的ε-PL防治苹果采后灰霉病的效果最佳，其能诱导苹果酚类化合物、类黄酮和木质素含量显著升高(P<0.05)，同时能诱导提高多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)和过氧化氢酶(CAT)编码基因的表达，提高抗性酶活性。此外，该浓度 ε-PL浸果处理0.25 h贮藏50 d后，苹果的自然腐烂率相对于对照组降低了77.26%，贮藏品质相比对照组更好。因此，ε-PL能有效控制苹果采后灰霉病，具有较高的应用价值。
Abstract:
-

参考文献/References:

［1］窦勇. ε-聚赖氨酸对苹果采后青霉病的控制及其机制研究［D］. 镇江：江苏大学，2023.
［2］Dou Y，Routledge M N，Gong Y Y，et al. Efficacy of epsilon-poly-L-lysine inhibition of postharvest blue mold in apples and potential mechanisms［J］. Postharvest Biology and Technology，2021，171：111346.
［3］Sun J Z，Chen H B，Chen Y H，et al. ε-Poly-L-lysine enhances fruit disease resistance in postharvest longans (Dimocarpus longan Lour.) by modulating energy status and ATPase activity［J］. Foods，2022，11(5)：773.
［4］Shu C，Cui K B，Li Q，et al. Epsilon-poly-L-lysine (ε-PL) exhibits multifaceted antifungal mechanisms of action that control postharvest Alternaria rot［J］. International Journal of Food Microbiology，2021，348：109224.
［5］Jiao W X，Liu X，Chen Q M，et al. Epsilon-poly-L-lysine (ε-PL) exhibits antifungal activity in vivo and in vitro against Botrytis cinerea and mechanism involved［J］. Postharvest Biology and Technology，2020，168：111270.
［6］Liu H，Zhao X X，Yu M，et al. Transcriptomic and functional analyses indicate novel anti-viral mode of actions on tobacco mosaic virus of a microbial natural product ε-poly-L-lysine［J］. Journal of Agricultural and Food Chemistry，2021，69(7)：2076-2086.
［7］Sun J Z，Fan Z Q，Chen Y Z，et al. The effect of ε-poly-L-lysine treatment on molecular，physiological and biochemical indicators related to resistance in longan fruit infected by Phomopsis longanae Chi［J］. Food Chemistry，2023，416：135784.
［8］Li H，He C，Li G J，et al. The modes of action of epsilon-polylysine (ε-PL) against Botrytis cinerea in jujube fruit［J］. Postharvest Biology and Technology，2019，147：1-9.
［9］王曼，魏佳，伊丽达娜·迪力夏提，等. 二氧化硫调控亚硫酸盐代谢对葡萄采后灰霉病的抑制作用［J］. 中国食品学报，2022，22(11)：299-308.
［10］Dou Y，Solairaj D，Guillaume L N N，et al. Transcriptome analysis provides insights into potential mechanisms of epsilon-poly-L-lysine inhibiting Penicillium expansum invading apples［J］. Postharvest Biology and Technology，2024，207：112622.
［11］An J X，Zhang B Q，Liang H J，et al. Antifungal activity and putative mechanism of HWY-289，a semisynthetic protoberberine derivative，against Botrytis cinerea［J］. Journal of Agricultural and Food Chemistry，2024，72(14)：7716-7726.
［12］Ayub J，Tahir A T，Iqbal U，et al. Effective biological control of lentil (Lens culinaris) Fusarium wilt and plant growth promotion through native rhizobacteria［J］. Physiological and Molecular Plant Pathology，2024，129：102203.
［13］Zhao L N，Wang Y J，Wang Y，et al. Effect of β-glucan on the biocontrol efficacy of Cryptococcus podzolicus against postharvest decay of pears and the possible mechanisms involved［J］. Postharvest Biology and Technology，2020，160：111057.
［14］Godana E A，Yang Q Y，Zhao L N，et al. Pichia anomala induced with chitosan triggers defense response of table grapes against post-harvest blue mold disease［J］. Frontiers in Microbiology，2021，12：704519.
［15］Xi Y N，Yang Q Y，Godana E A，et al. Study on the effect of Debaryomyces hansenii enhanced by alginate oligosaccharide against postharvest blue mold decay of apples and the physiological mechanisms involved［J］. Biological Control，2022，176：1049-9644.
［16］Godana E A，Zhang X Y，Yang Q Y，et al. Yarrowia lipolytica increased the activities of disease defense related enzymes and anti-fungal compounds in asparagus (Asparagus officinalis)［J］. Pesticide Biochemistry and Physiology，2024，198：105748.
［17］Zhou Y L，Zhao L N，Chen Y Q，et al. Study on the control effect and physiological mechanism of Wickerhamomyces anomalus on primary postharvest diseases of peach fruit［J］. International Journal of Food Microbiology，2024，413：110575.
［18］Zhou Y L，Zhao L N，Yue S Y，et al. Assay for transposase accessible-chromatin with high throughput sequencing (ATAC-seq) analysis the molecular responses of postharvest pear during Penicillium expansum infection［J］. Postharvest Biology and Technology，2024，209：112733.
［19］Deng L L，Zeng K F，Zhou Y H，et al. Effects of postharvest oligochitosan treatment on anthracnose disease in citrus (Citrus sinensis L. Osbeck) fruit［J］. European Food Research and Technology，2015，240：795-804.
［20］Morrison I M. A semi-micro method from the determination of lignin and its use in predicting the digestibility of forage crops［J］. Journal of the Science of Food and Agriculture，1972，23(4)：455-463.
［21］彭贞贞，叶旗慧，徐晓艳，等. 1-甲基环丙烯处理对红富士苹果贮藏品质的影响［J］. 浙江大学学报(农业与生命科学版)，2020，46(1)：83-92.
［22］Semyalo D，Kwon O，Wakholi C，et al. Nondestructive online measurement of pineapple maturity and soluble solids content using visible and near-infrared spectral analysis［J］. Postharvest Biology and Technology，2024，209(6)：112706.
［23］Tokala V Y，Singh Z，Kyaw P N. Postharvest fruit quality of apple influenced by ethylene antagonist fumigation and ozonized cold storage［J］. Food Chemistry，2021，341：128293.
［24］Wang Z D，Asghari M，Zahedipour-Sheshglani P，et al. Impact of 24-epibrassinoliode and methyl jasmonate on quality of red delicious apples［J］. Journal of the Science of Food and Agriculture，2024，104(3)：1621-1629.
［25］Li T T，Shi D D，Wu Q X，et al. Sodium para-aminosalicylate delays pericarp browning of litchi fruit by inhibiting ROS-mediated senescence during postharvest storage［J］. Food Chemistry，2019，278：552-559.
［26］Lima C P，Oster A H，Cavalcanti F R，et al. Induction of defense in apples by sulfated and deacetylated chichá gum［J］. Polímeros，2021，31(1)：e2021010.
［27］Li F J，Zhang X Y，Wang J H，et al. Preharvest application of 1-methylcyclopropene and ethephon altered cuticular wax biosynthesis and fruit quality of apples at harvest and during cold storage［J］. Horticultural Plant Journal，2022，8(2)：143-152.
［28］Hasanuzzanan M，Borhannuddin Bhuyan M H M，Zulfiqar F，et al. Reactive oxygen species and antioxidant defense in plants under abiotic stress：revisiting the crucial role of a universal defense regulator［J］. Antioxidants，2020，9(8)：681.
［29］Bai J L，Wang H H，Zhang J M，et al. Postharvest quality maintenance of wax apple and guava fruits by use of a fermented broth of an ε-poly-L-lysine producing Streptomyces strain［J］. PLoS One，2022，17(3)：e0265457.
［30］Ge Y H，Wei M L，Li C Y，et al. Reactive oxygen species metabolism and phenylpropanoid pathway involved in disease resistance against Penicillium expansum in apple fruit induced by ε-poly-L-lysine［J］. Journal of the Science of Food and Agriculture，2018，98(13)：5082-5088.

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备注/Memo

备注/Memo:
收稿日期：2024-05-26
基金项目：2024年江苏高校“青蓝工程”中青年学术带头人项目。
作者简介：窦勇(1979—)，安徽巢湖人，博士，副教授，研究方向为果蔬采后保鲜技术。E-mail：douyong1979@163.com。
更新日期/Last Update: 2024-12-05