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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第52卷

期数:

2024年第24期

页码:

44-52

栏目:

生物技术

出版日期:

2024-12-20

文章信息/Info

Title:

Transcriptomic analysis of leaf response to salt stress in barley near isogene lines with salt tolerance

作者:

李璐飞; 孙成群; 张宇航; 张萌娜; 洪益; 许如根; 朱娟

扬州大学农学院/江苏省作物基因组学和分子育种重点实验室/植物功能基因组学教育部重点实验室/江苏省作物遗传生理重点实验室/江苏省粮食作物现代产业技术协同创新中心，江苏扬州 225009

Author(s):

Li Lufei; et al

关键词:

大麦; 盐胁迫; 转录组; 差异表达基因; KEGG富集; 候选基因

Keywords:

-

分类号:

S512.301；S512.303

DOI:

-

文献标志码:

A

摘要:

为解析大麦耐盐基因QSl.TxNn.2H响应盐胁迫的分子机制，以包含耐盐基因QSl.TxNn.2H的1对近等基因系N53(盐敏感)和T66(耐盐)为供试材料，通过转录组测序(RNA-Seq)分析对照和盐胁迫(300 mmol/L NaCl)处理120 h的近等基因系叶片中响应盐胁迫的差异表达基因，根据测序结果进行DEG筛选、GO富集和KEGG分析，并使用qPCR技术分析基因的表达水平，验证转录组测序结果。研究表明，从N53中共鉴定到6 652个DEG，其中3 348个基因上调，3 304个基因下调；T66中只鉴定到1 447个DEG，716个基因上调，731个基因下调。GO富集表明，DEG主要富集在细胞过程、代谢过程、转运蛋白活性、细胞膜等。KEGG富集表明，耐盐系T66中特异性富集激素信号转导和植物MAPK信号通路，并筛选到12个在近等基因系间差异表达的基因。使用qPCR技术分析了盐胁迫中与离子运输相关的HvHKT、HvNHX和HvVHP基因的表达水平，结果与转录组里基因表达趋势一致，验证了转录组数据的准确性。QSl.TxNn.2H可能通过调控离子相关基因如HvNHX1、HvNHX4、HvHTK1.3、HvHTK2.2、HvVHP1.2和HvVHP1.1的表达调控大麦耐盐性。此外，在QSl.TxNn.2H定位区间内，有10个DEG，为QSl.TxNn.2H候选基因鉴定提供了参考。研究结果初步探索了耐盐基因QSl.TxNn.2H调控大麦耐盐性的分子机制，并分析了QSl.TxNn.2H定位区间内的DEG，为大麦耐盐育种提供了候选基因及理论依据。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2024-09-12
基金项目：江苏省高等学校大学生创新创业训练计划(编号：202411117241Y)；国家自然科学基金(编号：32301871)；省部共建青稞和牦牛种质资源与遗传改良国家重点实验室开放课题(编号：XZNKY-CZ-2024-02)；江苏省重点及面上项目(编号：BE2023334)；国家大麦青稞产业技术体系建设专项(编号：CARS-05)；江苏高校优势学科建设工程项目。
作者简介：李璐飞(2002—)，男，山西晋中人，主要从事大麦遗传育种研究。E-mail：2711607287@qq.com。
通信作者：朱娟，博士，讲师，主要从事大麦遗传育种研究。E-mail:007670@yzu.edu.cn。

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更新日期/Last Update: 2024-12-20