[1]徐勇,马远潇,梁悦,等.玫瑰花瓣表皮分泌细胞的显微观察及其与精油含量的关系[J].江苏农业科学,2024,52(24):148-155.
Xu Yong,et al.Microscopic observation of epidermal secretory cells of Rosa rugosa petals and their relationship with essential oil content[J].Jiangsu Agricultural Sciences,2024,52(24):148-155.
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玫瑰花瓣表皮分泌细胞的显微观察及其与精油含量的关系(
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
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第52卷
- 期数:
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2024年第24期
- 页码:
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148-155
- 栏目:
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园艺与林学
- 出版日期:
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2024-12-20
文章信息/Info
- Title:
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Microscopic observation of epidermal secretory cells of Rosa rugosa petals and their relationship with essential oil content
- 作者:
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徐勇1; 马远潇1; 梁悦1; 董桂芝2; 白梦娟1; 冯立国1
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1.扬州大学园艺园林学院,江苏扬州 225000; 2.平阴县玫瑰研究所,山东平阴 250407
- Author(s):
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Xu Yong; et al
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- 关键词:
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表皮分泌细胞; 油面积; 精油含量; 相关性
- Keywords:
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- 分类号:
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TQ645.1;O657.63;S685.120.1
- DOI:
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- 文献标志码:
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A
- 摘要:
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玫瑰精油提取的常规方法鉴定玫瑰品种精油含量高低需要大量的玫瑰花,不适用于玫瑰新品种的初步筛选,因此高油玫瑰新品种选育相关技术研究与开发对玫瑰资源选育及产业发展具有关键作用。基于玫瑰花瓣表皮分泌细胞是玫瑰精油合成的主要场所,选取19个同一地区栽培的玫瑰品种,开展玫瑰精油含量与表皮分泌细胞内油面积的相关关系研究。通过透射电镜观察染色后瑰盛开期时的花瓣上表皮分泌细胞可知,花瓣上表皮分泌细胞大多呈椭圆形,仅存的大液泡中有大量的嗜锇油团块状或条块状沿细胞壁分布。重瓣白玫瑰(R.rugosa cv. plena f.alba)等5个玫瑰品种的上表皮分泌细胞中存在较多的嗜锇油,油面积较大(106.6~184.4 μm2)。西胡2号(R.rugosa Xihu Erhao)等7个品种的油面积处于中等水平(45.8~77.4 μm2)。汉莎玫瑰(R.rugosa Hansha)等7个品种的油面积较低(5.8~44.7 μm2),其中大马士革玫瑰(R.damascena)和大马士革白玫瑰(R.damascena f.alba)中存在较多的淀粉粒。通过水蒸汽蒸馏法提取获得各玫瑰品种的精油,不同品种之间的出油量差异较大,其中出油量大于 0.3 μL/g 的包括平阴重瓣白玫瑰等4个品种。最后,对19个玫瑰品种花瓣上表皮分泌出油量和细胞内油面积的相关性进行分析表明,二者呈显著正相关关系(P<0.05),拟合的线性模型为y=0.001 5x+0.020 0。各品种玫瑰精油均以醇类、烷烃类、酚类和酯类为主,但各类型相对含量与油面积大小不存在显著相关关系。此研究能够用于定量预测玫瑰新品种中的精油总量,可作为高油玫瑰新品种选育的细胞学方法。
- Abstract:
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参考文献/References:
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备注/Memo
- 备注/Memo:
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收稿日期:2023-12-22
基金项目:国家自然科学基金(编号:32272749)。
作者简介:徐勇(1986—),男,河南信阳人,博士,讲师,主要从事观赏植物种质资源评价与应用研究。E-mail:yongxu@yzu.edu.cn。
通信作者:冯立国,博士,教授,主要从事观赏植物种质资源与分子生物学研究。E-mail:lgfeng@yzu.edu.cn。
更新日期/Last Update:
2024-12-20