[1]唐思源,刘欣,姚友礼.水稻内参基因的研究进展[J].江苏农业科学,2025,53(2):7-13.
　Tang Siyuan,et al.Research progress of reference genes in rice[J].Jiangsu Agricultural Sciences,2025,53(2):7-13.
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水稻内参基因的研究进展()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第2期
页码:
7-13
栏目:
专论与综述
出版日期:
2025-01-20

文章信息/Info

Title:
Research progress of reference genes in rice
作者:
唐思源; 刘欣; 姚友礼
扬州大学江苏省作物遗传生理重点实验室培育点/农业部长江中下游作物生理生态与栽培重点开放实验室/
粮食作物现代产业技术协同创新中心，江苏扬州 225009
Author(s):
Tang Siyuan; et al
关键词:
内参基因; RT-qPCR; 水稻; 基因表达
Keywords:
-
分类号:
S511.01
DOI:
-
文献标志码:
A
摘要:
实时荧光定量PCR(RT-qPCR)技术可为目的基因表达水平提供较为准确可靠的检测结果，但量化目的基因表达水平时需要使用内参基因来计算，因此，内参基因的选择合理与否会影响目的基因试验结果的准确性。理想的内参基因应该在所研究生物体内的大多数细胞中具有一致的较高丰度的转录表达，且表达水平不受外界因素的影响。实际上这些内参基因可能受物种、品种、发育阶段、器官以及处理条件等因素的影响而表达水平发生显著改变，从而影响定量计算结果。随着转录组测序技术的大量应用和数据积累，候选内参基因的选择也不再局限于传统的经验内参基因，利用公共数据库进行候选内参基因的筛选逐渐成为一种可能，因此，开展水稻内参基因筛选的相关研究具有重要的实践应用价值。本文综述总结了内参基因的来源和后续的筛选与验证方法，以及近几年来水稻内参基因由传统的管家基因向高通量测序数据中筛选出的适应水稻不同处理和组织的合适内参基因的转变，并对未来水稻内参基因的研究方向进行了展望，旨在为提高水稻基因表达研究的准确性提供有价值的应用参考。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-12-21
基金项目：江苏现代农业重点研发计划(编号：BE2022335)；扬州现代农业项目(编号：YZ2022043)。
作者简介：唐思源(2001—)，男，江苏盐城人，硕士研究生，研究方向为水稻生长发育的分子机理。E-mail：1295612145@qq.com。
通信作者：姚友礼，教授，主要从事水稻生长发育对环境响应的分子机理研究。E-mail：yaoyl@yzu.edu.cn。
更新日期/Last Update: 2025-01-20