[1]李竹梅,陈咀牛,何斐,等.花魔芋GRAS家族基因全基因组鉴定与胁迫下表达分析[J].江苏农业科学,2025,53(4):40-53.
 Li Zhumei,et al.Genome-wide identification and expression analysis of GRAS family genes in Amorphophallus konjac[J].Jiangsu Agricultural Sciences,2025,53(4):40-53.
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花魔芋GRAS家族基因全基因组鉴定与胁迫下表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第4期
页码:
40-53
栏目:
抗病基因
出版日期:
2025-02-20

文章信息/Info

Title:
Genome-wide identification and expression analysis of GRAS family genes in Amorphophallus konjac
作者:
李竹梅1陈咀牛1何斐23褚洪龙1
1.曲靖师范学院生物资源与食品工程学院,云南曲靖 655011; 2.安康学院现代农业与生物科技学院,陕西安康 725000; 3.安康学院富硒魔芋陕西省高校工程研究中心,陕西安康 725000
Author(s):
Li Zhumeiet al
关键词:
花魔芋GRAS家族基因逆境响应生物信息学分析
Keywords:
-
分类号:
Q943.2;S632.301
DOI:
-
文献标志码:
A
摘要:
GRAS家族是一类植物特有的转录因子,在植物生长发育和逆境响应等多个生物过程中发挥重要作用。花魔芋(Amorphophallus konjac)是我国重要的经济作物,目前尚无关于花魔芋GRAS家族基因的相关报道。采用生物信息学手段对花魔芋GRAS家族基因进行全基因组鉴定与功能分析,通过RNA-Seq解析了花魔芋GRAS家族基因响应非生物和生物胁迫胁迫的表达情况。结果显示,花魔芋有51个GRAS家族成员,划分为8个亚家族。该家族编码的蛋白由144~850个氨基酸组成,分子量介于15 789.78~90 823.32 u之间,等电点在4.94~11.19之间,大部分AkGRAS编码蛋白为酸性不稳定蛋白。顺式作用元件分析显示AkGRAS基因的启动子区富含激素、干旱和低温等逆境响应元件。RNA-seq结果表明,多个亚家族基因成员响应干旱、盐胁迫和软腐病原菌侵染表达。且PAT1亚家族基因AkGRAS35、AkGRAS19、AkGRAS40在盐胁迫下显著上调表达,AkGRAS41显著下调表达;AkGRAS19、AkGRAS20、AkGRAS35、AkGRAS51在软腐病原菌侵染下上调表达,AkGRAS8AkGRAS46下调表达。推测PAT1亚家族基因在花魔芋高盐胁迫和软腐病原菌侵染应答中发挥重要作用。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-11-06
基金项目:国家自然科学基金(编号:31860057);云南省基础研究专项面上项目(编号:202401AT070002);安康市科技计划(编号:AK2022-NY-04);安康学院专项计划(编号:2023AYKCYZ05);曲靖师范学院博士创新团队支持计划。
作者简介:李竹梅(1988—),女,云南腾冲人,博士,讲师,主要从事植物与微生物互作研究。E-mail:lizhume@163.com。
通信作者:褚洪龙,博士,副教授,从事植物抗逆生理、植物与微生物互作研究。E-mail:chuhonglo@163.com。
更新日期/Last Update: 2025-02-20