[1]常璐,方正武,高利.小麦光腥黑粉菌效应蛋白g2164的生物信息学分析、亚细胞定位及毒性验证[J].江苏农业科学,2025,53(9):25-32.
 Chang Lu,et al.Bioinformatics analysis,subcellular localization and toxicity verification of effector protein g2164 of Tilletia foetida[J].Jiangsu Agricultural Sciences,2025,53(9):25-32.
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小麦光腥黑粉菌效应蛋白g2164的生物信息学分析、亚细胞定位及毒性验证()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第9期
页码:
25-32
栏目:
生物技术
出版日期:
2025-05-05

文章信息/Info

Title:
Bioinformatics analysis,subcellular localization and toxicity verification of effector protein g2164 of Tilletia foetida
作者:
常璐12方正武1高利23
1.长江大学农学院,湖北荆州 451199; 2.新疆农业科学院植物保护研究所/农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室,新疆乌鲁木齐 830091; 3.中国农业科学院植物保护研究所/植物病虫害综合治理全国重点实验室,北京 100193
Author(s):
Chang Luet al
关键词:
小麦光腥黑粉菌效应蛋白生物信息学分析亚细胞定位毒性验证
Keywords:
-
分类号:
S435.121.4+9
DOI:
-
文献标志码:
A
摘要:
为了初步探究小麦光腥黑粉菌(Tilletia foetida)入侵小麦的侵染机制,从小麦光腥黑粉菌在入侵宿主时所分泌的效应蛋白入手,在小麦光腥黑粉菌的转录组数据测序结果中筛选出了含有特殊结构的效应蛋白g2164。由于病原真菌的效应蛋白随着寄主的不同,所表现出的基序以及结构域随之不同,具有很强的特异性,因此,为了解小麦光腥黑粉菌基因g2164所编码的效应蛋白的生物学功能,通过聚合酶链式反应技术获得g2164基因cDNA的全长序列,对其进行生物信息学分析解析该蛋白理化性质,通过毒性验证试验验证其蛋白毒性,进行亚细胞定位试验对该基因功能行使区域进行定位分析。生物信息学分析结果显示,g2164基因全长1 365 bp,共编码454个氨基酸,相对分子质量51 964.70,理论等电点为9.22;具有典型的Bg1C家族结构域;其蛋白的不稳定系数为33.64,平均亲水性系数为-0.531,是一种亲水性稳定的蛋白;使用重组法,将g2164基因整合到pBin-GFP载体和pGR-107载体上,再将它们转到根癌农杆菌Agrobacterium tumefaciens GV3101中,使用本氏烟草进行亚细胞定位试验,结果显示,g2164在细胞核和细胞膜上定位;毒性验证试验表明,该基因不具备抑制细胞坏死的活性。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2024-05-25
基金项目:新疆维吾尔自治区重大科技专项(编号:2023A02009)。
作者简介:常璐(1998—),女,安徽蚌埠人,硕士研究生,研究方向为麦类真菌病害研究。E-mail:2110774816@qq.com。
通信作者:方正武,博士,教授,研究方向为作物种质资源创新与利用,E-mail:fangzhengwu88@163.com;高利,博士,研究员,研究方向为禾谷类黑粉病,E-mail:xiaogaosx@hotmail.com。
更新日期/Last Update: 2025-05-05