[1]赵鹤,王迎新,邹霈怡,等.狗枣猕猴桃AkbHLH92基因生物信息学及表达特性分析[J].江苏农业科学,2025,53(15):70-77.
 Zhao He,et al.Bioinformatics and expression analysis of AkbHLH92 gene in Actinidia kolomikta[J].Jiangsu Agricultural Sciences,2025,53(15):70-77.
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狗枣猕猴桃AkbHLH92基因生物信息学及表达特性分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第15期
页码:
70-77
栏目:
生物技术
出版日期:
2025-08-05

文章信息/Info

Title:
Bioinformatics and expression analysis of AkbHLH92 gene in Actinidia kolomikta
作者:
赵鹤王迎新邹霈怡刘美艳黄啟燕李然红
牡丹江师范学院生命科学与技术学院,黑龙江牡丹江 157011
Author(s):
Zhao Heet al
关键词:
狗枣猕猴桃AkbHLH92基因克隆亚细胞定位组织表达特异性非生物胁迫
Keywords:
-
分类号:
S188;S663.401
DOI:
-
文献标志码:
A
摘要:
为了揭示狗枣猕猴桃的抗寒机制,基于前期低温处理的狗枣猕猴桃转录组数据,分析bHLH家族基因对低温的响应情况,其中bHLH家族成员AkbHLH92上调显著。利用酶切酶连的方法克隆AkbHLH92并构建亚细胞定位重组载体pBI121-AkbHLH92-GFP,利用生物信息学方法分析蛋白质理化性质、跨膜结构域、生物系统发育等,通过相对荧光定量PCR(RT-qPCR)技术分析AkbHLH92的表达模式。结果表明,AkbHLH92基因全长693 bp,编码蛋白为亲水性蛋白;AkbHLH92二级结构主要由α螺旋和不规则卷曲构成;系统发育分析结果表明,狗枣猕猴桃与毛花猕猴桃的亲缘关系较近;AkbHLH92编码的蛋白质定位于细胞核和细胞质;AkbHLH92基因在植物茎和叶中表达量较高,在根中表达量较低,在花和果实中均无表达;AkbHLH92对不同非生物胁迫响应不同,在4 ℃低温胁迫的48 h内,AkbHLH92的相对表达量呈逐步上升的趋势,处理48 h时达到最高,为对照的5.1倍;盐胁迫下,AkbHLH92的相对表达量显著下调,处理24 h时,相对表达量最低,为对照的0.007 9倍;水淹胁迫下,AkbHLH92的相对表达量呈先升再降的趋势,在处理 12 h 时达到峰值,为对照的2.914倍。狗枣猕猴桃AkbHLH92基因积极响应非生物胁迫,可能在其抗逆过程中具有重要作用。
Abstract:
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参考文献/References:

[1]刘丹,毕德泷,陈鑫,等. 狗枣猕猴桃胚胎发育晚期富集蛋白的生物信息学分析[J]. 江苏农业科学,2019,47(24):30-33.
[2]贾佳林,刘香苏,安娇,等. 长白山野生狗枣猕猴桃种质资源的数量性状变异分析及分级指标探讨[J]. 延边大学农学学报,2019,41(1):1-7.
[3]Guo X J,Wang J R. Global identification,structural analysis and expression characterization of bHLH transcription factors in wheat[J]. BMC Plant Biology,2017,17(1):90.
[4]Chu Y,Xiao S M,Su H,et al. Genome-wide characterization and analysis of bHLH transcription factors in Panax ginseng[J]. Acta Pharmaceutica Sinica B,2018,8(4):666-677.
[5]Radani Y,Li R X,Korboe H M,et al. Transcriptional and post-translational regulation of plant bHLH transcription factors during the response to environmental stresses[J]. Plants,2023,12(11):2113.
[6]Toledo-Ortiz G,Huq E,Quail P H. The Arabidopsis basic/helix-loop-helix transcription factor family[J]. The Plant Cell,2003,15(8):1749-1770.
[7]Fairman R,Beran-Steed R K,Anthony-Cahill S J,et al. Multiple oligomeric states regulate the DNA binding of helix-loop-helix peptides[J]. Proceedings of the National Academy of Sciences of the United States of America,1993,90(22):10429-10433.
[8]Zhao L F,Bi W Y,Jia Y Q,et al. Genome-wide characterization of bHLH family genes and expression analysis in response to osmotic stress in Betula platyphylla[J]. Plants,2023,12(21):3687.
[9]Sun H,Fan H J,Ling H Q. Genome-wide identification and characterization of the bHLH gene family in tomato[J]. BMC Genomics,2015,16(1):9.
[10]Wang R Q,Zhao P,Kong N N,et al. Genome-wide identification and characterization of the potato bHLH transcription factor family[J]. Genes,2018,9(1):54.
[11]李欣,李影,曲子越,等. bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制[J]. 植物生理学报,2017,53(1):1-8.
[12]张凯伦,罗祖良,郭玉华,等. bHLH转录因子调控药用植物萜类化合物生物合成的研究进展[J]. 中国现代中药,2017,19(1):142-147.
[13]包雪梅,胡娜,宗渊,等. 转录组学挖掘黑果枸杞中花青素生物合成代谢关键bHLH调控基因[J]. 分子植物育种,2023,21(6):1864-1873.
[14]郑珍珍,陈雪雪,沈元月,等. 转录因子FabHLH148参与草莓果实的颜色发育[J]. 果树学报,2022,39(8):1358-1367.
[15]Liang G,Zhang H M,Li X L,et al. bHLH transcription factor bHLH115 regulates iron homeostasis in Arabidopsis thaliana[J]. Journal of Experimental Botany,2017,68(7):1743-1755.
[16]Gu X Y,Gao S X,Li J,et al. The bHLH transcription factor regulated gene OsWIH2 is a positive regulator of drought tolerance in rice[J]. Plant Physiology and Biochemistry,2021,169:269-279.
[17]Ji X Y,Nie X G,Liu Y J,et al. A bHLH gene from Tamarix hispida improves abiotic stress tolerance by enhancing osmotic potential and decreasing reactive oxygen species accumulation[J]. Tree Physiology,2016,36(2):193-207.
[18]Li J L,Wang T,Han J,et al. Genome-wide identification and characterization of cucumber bHLH family genes and the functional characterization of CsbHLH041 in NaCl and ABA tolerance in Arabidopsis and cucumber[J]. BMC Plant Biology,2020,20(1):272.
[19]Wang Z Y,Zhang Y M,Hu H F,et al. CabHLH79 acts upstream of CaNAC035 to regulate cold stress in pepper[J]. International Journal of Molecular Sciences,2022,23(5):2537.
[20]齐学礼,李莹,李春盈,等. bHLH转录因子在植物耐冷基因工程中的应用进展[J]. 河南农业科学,2023,52(11):1-9.
[21]Payne C T,Zhang F,Lloyd A M. GL3 encodes a bHLH protein that regulates trichome development in Arabidopsis through interaction with GL1 and TTG1[J]. Genetics,2000,156(3):1349-1362.
[22]Gil-Muoz F,Sánchez-Navarro J A,Besada C,et al. MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit[J]. Scientific Reports,2020,10(1):3543.
[23]Zhu Z G,Chen G P,Guo X H,et al. Overexpression of SlPRE2,an atypical bHLH transcription factor,affects plant morphology and fruit pigment accumulation in tomato[J]. Scientific Reports,2017,7(1):5786.
[24]Li Z X,Liu C,Zhang Y,et al. The bHLH family member ZmPTF1 regulates drought tolerance in maize by promoting root development and abscisic acid synthesis[J]. Journal of Experimental Botany,2019,70(19):5471-5486.
[25]Zhao P C,Li X X,Jia J T,et al. bHLH92 from sheepgrass acts as a negative regulator of anthocyanin/proanthocyandin accumulation and influences seed dormancy[J]. Journal of Experimental Botany,2019,70(1):269-284.
[26]Jiang Y Q,Yang B,Deyholos M K. Functional characterization of the Arabidopsis bHLH92 transcription factor in abiotic stress[J]. Molecular Genetics and Genomics,2009,282(5):503-516.
[27]Ni L J,Wang Z Q,Fu Z K,et al. Genome-wide analysis of basic helix-loop-helix family genes and expression analysis in response to drought and salt stresses in Hibiscus hamabo sieb.et zucc[J]. International Journal of Molecular Sciences,2021,22(16):8748.

相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2024-08-21
基金项目:黑龙江省省属本科高校优秀青年教师基础研究支持计划(编号:YQJH2023094);牡丹江师范学院“十四五”双一流学科建设揭榜挂帅项目(编号:MSYSYL2022015);牡丹江师范学院研究生科技创新项目(编号:kjcx2023-116mdjnu)。
作者简介:赵鹤(1998—),女,黑龙江哈尔滨人,硕士研究生,主要从事植物遗传学研究。E-mail:sxwzh123@126.com。
通信作者:李然红,博士,教授,硕士生导师,主要从事林木遗传育种研究。E-mail:swxlrh@126.com。
更新日期/Last Update: 2025-08-05