[1]章加应,安海山,李水根,等.低温胁迫下枇杷抗寒生理指标变化规律及关键候选基因的筛选[J].江苏农业科学,2025,53(22):148-156.
　Zhang Jiaying,et al.Change of physiological indicator related to cold tolerance in loquat under cold stress and screening of key candidate genes[J].Jiangsu Agricultural Sciences,2025,53(22):148-156.
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低温胁迫下枇杷抗寒生理指标变化规律及关键候选基因的筛选()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
148-156
栏目:
园艺与林学
出版日期:
2025-11-20

文章信息/Info

Title:
Change of physiological indicator related to cold tolerance in loquat under cold stress and screening of key candidate genes
作者:
章加应; 安海山; 李水根; 张学英
上海市农业科学院林木果树研究所/上海市设施园艺技术重点实验室，上海 201403
Author(s):
Zhang Jiaying; et al
关键词:
枇杷; 花序; 低温胁迫; 生理指标; 差异表达基因; 关键候选基因
Keywords:
-
分类号:
S667.301
DOI:
-
文献标志码:
A
摘要:
为探究枇杷不同品种对低温胁迫的生理响应和分子调控机制，以枇杷耐寒型品种火炬和低温敏感型品种宁海白为试材，选取长势一致的花序在人工模拟的-4 ℃低温胁迫条件下测定不同处理时间的相关抗寒生理生化指标。同时，结合前期转录组数据筛选到的差异表达基因，分析其在低温胁迫处理不同时间的耐寒型和低温敏感型枇杷品种中的表达模式，筛选与枇杷抗寒性相关的关键候选基因。低温胁迫处理下，低温敏感型枇杷品种宁海白花序先与耐寒型品种火炬表现出褐化，相对电导率、半致死温度(LT50)和冻害率明显高于火炬。火炬花序中相关抗寒生理指标(可溶性糖和脯氨酸)值高于宁海白花序并具有更强的抗氧化酶活性和活性氧清除能力。结合转录组数据筛选的差异表达基因(EjUGT88、EjSTP13、EjGT10、EjPERK9、EjPOD11、EjPOD47)表达模式与相关抗寒生理指标表现出一致的变化趋势，并在火炬花序中的表达水平整体上高于宁海白，表明差异表达基因可能在枇杷响应低温胁迫过程中发挥重要作用，可以作为与枇杷抗寒性相关的关键候选基因。研究结果为枇杷低温胁迫响应中特定基因的鉴定提供了宝贵的资源，并有助于阐明枇杷响应低温胁迫的分子调控机制。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2025-05-12
基金项目：上海市“科技创新行动计划”启明星项目(扬帆专项)(编号：23YF1439600)。
作者简介：章加应(1993—)，男，安徽望江人，硕士，助理研究员，主要从事枇杷生理栽培及抗寒机理研究。E-mail：jiayingzsaas@163.com。
通信作者：张学英，博士，研究员，主要从事特色水果栽培技术和遗传育种研究。E-mail：zhangxueying@saas.sh.cn。
更新日期/Last Update: 2025-11-20