[1]刘灿玉,陆信娟,樊继德,等.长期不同施肥类型对土壤理化性质和大蒜根际微生物群落的影响[J].江苏农业科学,2025,53(22):220-228.
 Liu Canyu,et al.Effects of longterm different fertilization types on soil physicochemical properties and rhizosphere microbial community of garlic[J].Jiangsu Agricultural Sciences,2025,53(22):220-228.
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长期不同施肥类型对土壤理化性质和大蒜根际微生物群落的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
220-228
栏目:
资源与环境
出版日期:
2025-11-20

文章信息/Info

Title:
Effects of longterm different fertilization types on soil physicochemical properties and rhizosphere microbial community of garlic
作者:
刘灿玉陆信娟樊继德赵永强张碧薇葛洁杨青青李梦倩刘光杨杨峰
江苏徐淮地区徐州农业科学研究所,江苏徐州 221131
Author(s):
Liu Canyuet al
关键词:
施肥类型土壤理化性质土壤酶大蒜根际微生物群落
Keywords:
-
分类号:
S633.406
DOI:
-
文献标志码:
A
摘要:
利用土壤长期定位试验田,设置纯施化肥(T1)、有机无机肥配施(T2)、纯施有机肥(1/2施氮量)(T3)3个处理,以不施氮肥为对照(CK),研究长期不同施肥类型对土壤理化性质和大蒜根际微生物群落的影响。结果表明,长期施肥后土壤pH值较试验前明显下降,且各处理间差异不显著。与CK相比,T1处理显著降低土壤有机质含量,而T2、T3处理显著增加土壤有机质和速效养分(N、P、K)含量;T1处理仅显著增加土壤纤维素酶和碱性磷酸酶活性,而T2处理显著诱导土壤蔗糖酶、蛋白酶、脲酶、纤维素酶及碱性磷酸酶活性的增加,T3处理显著增加土壤脲酶、蛋白酶及蔗糖酶活性。各处理对土壤真菌α多样性无显著影响,T1处理细菌α多样性显著低于其他处理;各处理间细菌和真菌菌群结构均存在显著差异,其中有机肥处理明显增加拟杆菌门的相对丰度;施氮肥处理组丝孢菌门的相对丰度下降,显花植物门的相对丰度增加。Pearson相关性分析发现,环境因子与土壤微生物丰度存在相关性;且基于VPA分析发现,脲酶活性对细菌门水平物种分布的影响最大,土壤pH值对真菌门水平物种分布的影响最大。综上,长期施肥会导致土壤酸化,且长期纯施化肥会造成土壤有机质含量下降、土壤细菌α多样性下降,添加有机肥有利于促进土壤养分含量和土壤酶活性的增加,提高有益菌群的相对丰度。
Abstract:
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参考文献/References:

[1]王宁,南宏宇,冯克云. 化肥减量配施有机肥对棉田土壤微生物生物量、酶活性和棉花产量的影响[J]. 应用生态学报,2020,31(1):173-181.
[2]刘恩科,赵秉强,李秀英,等. 长期施肥对土壤微生物量及土壤酶活性的影响[J]. 植物生态学报,2008,32(1):176-182.
[3]李琳,向丹,武亚芬,等. 长期不同施肥方式对日光温室番茄土壤养分和微生物群落结构的影响[J]. 应用生态学报,2022,33(2):415-422.
[4]Yang Y,Chen X L,Liu L X,et al. Nitrogen fertilization weakens the linkage between soil carbon and microbial diversity:a global meta-analysis[J]. Global Change Biology,2022,28(21):6446-6461.
[5]Ma L F,Yang X D,Shi Y Z,et al. Response of tea yield,quality and soil bacterial characteristics to long-term nitrogen fertilization in an eleven-year field experiment[J]. Applied Soil Ecology,2021,166:103976.
[6]Guo J H,Liu X J,Zhang Y,et al. Significant acidification in major Chinese croplands[J]. Science,2010,327(5968):1008-1010.
[7]颜明娟,陈玉真,林诚,等. 连续配施有机肥对茶园土壤细菌群落结构及功能类群的影响[J]. 农业环境科学学报,2024,43(12):2959-2973.
[8]袁丁. 基于Meta分析的中国长期施肥对土壤微生物性状的影响研究[D]. 南京:南京信息工程大学,2023:41.
[9]李桂龙,李朋发,吴萌,等. 化肥配施有机肥对花生根际细菌群落结构及共存网络的影响[J]. 土壤,2022,54(3):498-507.
[10]胡冬平,唐江华,徐文修,等. 氮肥配施有机肥对半干旱区旱地小麦土壤三大菌群和酶活性及产量的影响[J]. 南京农业大学学报,2025,48(1):144-151.
[11]Dang P F,Li C F,Lu C,et al. Effect of fertilizer management on the soil bacterial community in agroecosystems across the globe[J]. Agriculture,Ecosystems & Environment,2022,326:107795.
[12]刘东海,乔艳,李双来,等. 长期施肥对黄棕壤细菌多样性的影响[J]. 植物营养与肥料学报,2021,27(5):760-767.
[13]Ning Q,Chen L,Jia Z J,et al. Multiple long-term observations reveal a strategy for soil pH-dependent fertilization and fungal communities in support of agricultural production[J]. Agriculture,Ecosystems & Environment,2020,293:106837.
[14]Wang T,Chen H,Zhou W,et al. Garlic-rice system increases net economic benefits and reduces greenhouse gas emission intensity[J]. Agriculture,Ecosystems & Environment,2022,326:107778.
[15]汪兴汉,张爱民. 葱蒜类蔬菜生产关键技术百问百答[M]. 北京:中国农业出版社,2005:25.
[16]Ma Y J,Shen S Z,Wan C,et al. Organic fertilizer substitution over six years improves the productivity of garlic,bacterial diversity,and microbial communities network complexity[J]. Applied Soil Ecology,2023,182:104718.
[17]中华人民共和国农业部. 土壤检测第6部分:土壤有机质的测定:NY/T 1121.6—2006[S]. 北京:中国农业出版社,2006.
[18]中华人民共和国农业部. 土壤速效钾和缓效钾含量的测定:NY/T 889—2004[S]. 北京:中国农业出版社,2005.
[19]中华人民共和国农业部. 土壤检测第25部分:土壤有效磷的测定连续流动分析仪法:NY/T 1121.25—2012[S]. 北京:中国农业出版社,2012.
[20]国家林业局. 森林土壤氮的测定:LY/T 1228—2015[S]. 北京:中国标准出版社,2015.
[21]中华人民共和国农业部.土壤检测第2部分:土壤pH值的测定:NY/T 1121.2—2012 [S]. 北京:中国农业出版社,2012.
[22]肖力婷,杨慧林,黄文新,等. 生草栽培对南丰蜜橘园土壤酶活性及氮循环功能微生物的影响[J]. 应用与环境生物学报,2021,27(6):1476-1484.
[23]高大响,黄小忠,王亚萍. 秸秆还田及腐熟剂对土壤微生物特性和酶活性的影响[J]. 江苏农业科学,2016,44(12):468-471.
[24]宗莉,林红霞,苗宁涛,等. 矿物生物炭对马铃薯品质与土壤肥力的影响[J]. 农业环境科学学报,2025,44(4):1056-1065.
[25]焦晓光,魏丹,隋跃宇. 长期施肥对黑土和暗棕壤土壤酶活性及土壤养分的影响[J]. 土壤通报,2011,42(3):698-703.
[26]姜一凡,张瑞峰,田伟,等. 牛粪有机肥对土壤微生物群落的影响[J]. 青岛农业大学学报(自然科学版),2024,41(2):130-137.
[27]Lin W W,Lin M H,Zhou H Y,et al. The effects of chemical and organic fertilizer usage on rhizosphere soil in tea orchards[J]. PLoS One,2019,14(5):e0217018.
[28]Yi X Y,Ji L F,Hu Z M,et al. Organic amendments improved soil quality and reduced ecological risks of heavy metals in a long-term tea plantation field trial on an Alfisol[J]. Science of the Total Environment,2022,838:156017.
[29]Han Z Q,Lin H Y,Xu P S,et al. Impact of organic fertilizer substitution and biochar amendment on net greenhouse gas budget in a tea plantation[J]. Agriculture,Ecosystems & Environment,2022,326:107779.
[30]袁玲,杨邦俊,郑兰君,等. 长期施肥对土壤酶活性和氮磷养分的影响[J]. 植物营养与肥料学报,1997,3(4):300-306.
[31]Camenzind T,Httenschwiler S,Treseder K K,et al. Nutrient limitation of soil microbial processes in tropical forests[J]. Ecological Monographs,2018,88(1):4-21.
[32]梁文举,董元华,李英滨,等. 土壤健康的生物学表征与调控[J]. 应用生态学报,2021,32(2):719-728.
[33]Wang F H,Han W X,Chen S M,et al. Fifteen-year application of manure and chemical fertilizers differently impacts soil ARGs and microbial community structure[J]. Frontiers in Microbiology,2020,11:62.
[34]Sun R B,Dsouza M,Gilbert J A,et al. Fungal community composition in soils subjected to long-term chemical fertilization is most influenced by the type of organic matter[J]. Environmental Microbiology,2016,18(12):5137-5150.
[35]Gu S S,Hu Q L,Cheng Y Q,et al. Application of organic fertilizer improves microbial community diversity and alters microbial network structure in tea (Camellia sinensis) plantation soils[J]. Soil and Tillage Research,2019,195:104356.
[36]Zhang S N,Sun L T,Wang Y,et al. Cow manure application effectively regulates the soil bacterial community in tea plantation[J]. BMC Microbiology,2020,20(1):190.
[37]Wang J L,Liu K L,Zhao X Q,et al. Balanced fertilization over four decades has sustained soil microbial communities and improved soil fertility and rice productivity in red paddy soil[J]. Science of the Total Environment,2021,793:148664.
[38]Li P F,Liu M,Ma X Y,et al. Responses of microbial communities to a gradient of pig manure amendment in red paddy soils[J]. Science of the Total Environment,2020,705:135884.
[39]van der Heijden M G A,Bardgett R D,van Straalen N M. The unseen majority:soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems[J]. Ecology Letters,2008,11(3):296-310.

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备注/Memo

备注/Memo:
收稿日期:2024-10-23
基金项目:国家特色蔬菜产业技术体系建设专项(编号:CARS-24-A-07);江苏现代农业产业技术体系建设专项[编号:JATS(2023)050];徐州市政策引导类计划(产学研合作)项目(编号:KC22452)。
作者简介:刘灿玉(1988—),女,山东单县人,博士,助理研究员,研究方向为大蒜品种选育与配套绿色高效栽培技术。E-mail:liucanyu@126.com。
通信作者:杨峰,博士,研究员,研究方向为大蒜品种选育与配套绿色高效栽培技术。E-mail:xz-yangfeng@163.com。
更新日期/Last Update: 2025-11-20