[1]李颖,郭文涛,段高飞,等.月季DEADbox RNA解旋酶家族全基因组鉴定与表达分析[J].江苏农业科学,2026,54(8):59-70.
　Li Ying,et al.Genome wide Identification and expression analysis of DEADbox RNA helicase family in Rosa chinensis[J].Jiangsu Agricultural Sciences,2026,54(8):59-70.
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月季DEADbox RNA解旋酶家族全基因组鉴定与表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
59-70
栏目:
耐干旱基因鉴定
出版日期:
2026-04-20

文章信息/Info

Title:
Genome wide Identification and expression analysis of DEADbox RNA helicase family in Rosa chinensis
作者:
李颖; 郭文涛; 段高飞; 王艺霖; 许瑞瑞
潍坊学院生物与海洋学院，山东潍坊 261061
Author(s):
Li Ying; et al
关键词:
DEAD-box RNA解旋酶; 月季; 生物信息学; 表达分析
Keywords:
-
分类号:
S188；S685.120.1
DOI:
-
文献标志码:
A
摘要:
DEAD-box型RNA解旋酶作为最大的一类RNA解旋酶蛋白质亚家族，除具有RNA代谢调控功能外，还广泛参与了植物的生长发育和非生物胁迫响应。通过对月季全基因组的鉴定，筛选出40个DEAD-box RNA(DDX)解旋酶基因家族成员。利用生物信息学方法对月季DEAD-box RNA解旋酶基因家族进行系统的理化性质、染色体定位、共线性、系统进化树、顺式作用元件及基因表达模式等分析。结果表明，RcDEAD家族成员的蛋白序列长度为 411~1 138 aa，分子量为46.71~129.29 ku，等电点为5.07~10.00。大部分成员定位于细胞核，少部分成员定位于细胞质、叶绿体中。RcDEAD集中分布在7条染色体上，其中2号染色体分布数目最多。RcDEAD家族包含2个串联重复基因对及存在3对共线性关系的基因。所有家族成员均含有8个保守基序，且motif4(基序Ⅱ)中DEAD序列保守性达100%。25个RcDEAD成员含有SrmB结构域，15个RcDEAD成员含有DEADc结构域。系统进化树将RcDEAD成员分为6个亚组，其Group Ⅵ数量最多(占比44.28%)。月季DEAD解旋酶家族与烟草、拟南芥的进化关系最近，其次是小麦。共线性分析结果表明，月季与沙梨、枇杷、苹果之间存在较多的同源基因。顺式作用元件分析发现，RcDEAD家族成员启动子区分布大量光、激素、非生物胁迫响应等相关元件。组织表达模式分析结果显示，RcDEAD成员在根、茎、叶和皮刺中有不同程度的表达。干旱胁迫诱导表达分析结果表明，在根中有9个RcDEAD成员表达上调，20个成员表达下调；在叶片中有23个RcDEAD成员表达上调，11个成员表达下调。
Abstract:
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参考文献/References:

［1］Tauber D，Tauber G，Khong A，et al. Modulation of RNA condensation by the DEAD-box protein eIF4A［J］. Cell，2020，180(3)：411-426.
［2］Owttrim G W. RNA helicases and abiotic stress［J］. Nucleic Acids Research，2006，34(11)：3220-3230.
［3］Wilkinson M E，Charenton C，Nagai K. RNA splicing by the spliceosome［J］. Annual Review of Biochemistry，2020，89：359-388.
［4］Li X D，Li C H，Zhu J Y，et al. Functions and mechanisms of RNA helicases in plants［J］. Journal of Experimental Botany，2023，74(7)：2295-2310.
［5］Fairman-Williams M E，Guenther U P，Jankowsky E. SF1 and SF2 helicases：family matters［J］. Current Opinion in Structural Biology，2010，20(3)：313-324.
［6］Owttrim G W. RNA helicases：diverse roles in prokaryotic response to abiotic stress［J］. RNA Biology，2013，10(1)：96-110.
［7］Nguyen L V，Seok H Y，Woo D H，et al. Overexpression of the DEAD-box RNA helicase gene AtRH17 confers tolerance to salt stress in Arabidopsis［J］. International Journal of Molecular Sciences，2018，19(12)：3777.
［8］Gong Z Z，Lee H，Xiong L M，et al. RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance［J］. Proceedings of the National Academy of Sciences of the United States of America，2002，99(17)：11507-11512.
［9］Gong Z Z，Dong C H，Lee H，et al. A DEAD box RNA helicase is essential for mRNA export and important for development and stress responses in Arabidopsis［J］. The Plant Cell，2005，17(1)：256-267.
［10］Zhou M Q，Shen C，Wu L H，et al. CBF-dependent signaling pathway：a key responder to low temperature stress in plants［J］. Critical Reviews in Biotechnology，2011，31(2)：186-192.
［11］Seok H Y，Nguyen L V，van Nguyen D，et al. Investigation of a novel salt stress-responsive pathway mediated by Arabidopsis DEAD-box RNA helicase gene AtRH17 using RNA-seq analysis［J］. International Journal of Molecular Sciences，2020，21(5)：1595.
［12］Gu L L，Xu T，Lee K，et al. A chloroplast-localized DEAD-box RNA helicase AtRH3 is essential for intron splicing and plays an important role in the growth and stress response in Arabidopsis thaliana［J］. Plant Physiology and Biochemistry，2014，82：309-318.
［13］Kanai M，Hayashi M，Kondo M，et al. The plastidic DEAD-box RNA helicase 22，HS3，is essential for plastid functions both in seed development and in seedling growth［J］. Plant and Cell Physiology，2013，54(9)：1431-1440.
［14］Nishimura K，Ashida H，Ogawa T，et al. A DEAD box protein is required for formation of a hidden break in Arabidopsis chloroplast 23S rRNA［J］. The Plant Journal，2010，63(5)：766-777.
［15］Chantarachot T，Sorenson R S，Hummel M，et al. DHH1/DDX6-like RNA helicases maintain ephemeral half-lives of stress-response mRNAs［J］. Nature Plants，2020，6(6)：675-685.
［16］Huang C K，Huang L F，Huang J J，et al. A DEAD-box protein，AtRH36，is essential for female gametophyte development and is involved in rRNA biogenesis in Arabidopsis［J］. Plant & Cell Physiology，2010，51(5)：694-706.
［17］Liu M，Shi D Q，Yuan L，et al. SLOW WALKER3，encoding a putative DEAD-box RNA helicase，is essential for female gametogenesis in Arabidopsis［J］. Journal of Integrative Plant Biology，2010，52(9)：817-828.
［18］Wang Y C，Duby G，Purnelle B，et al. Tobacco VDL gene encodes a plastid DEAD box RNA helicase and is involved in chloroplast differentiation and plant morphogenesis［J］. The Plant Cell，2000，12(11)：2129.
［19］Li D Y，Liu H Z，Zhang H J，et al. OsBIRH1，a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress［J］. Journal of Experimental Botany，2008，59(8)：2133-2146.
［20］Li X W，Gao X Q，Wei Y，et al. Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5′-triphosphate-dependent RNA helicases regulates tapetum degeneration［J］. The Plant Cell，2011，23(4)：1416-1434.
［21］Nawaz G，Kang H. Rice OsRH58，a chloroplast DEAD-box RNA helicase，improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation［J］. BMC Plant Biology，2019，19(1)：17.
［22］Nawaz G，Lee K，Park S J，et al. A chloroplast-targeted cabbage DEAD-box RNA helicase BrRH22 confers abiotic stress tolerance to transgenic Arabidopsis plants by affecting translation of chloroplast transcripts［J］. Plant Physiology and Biochemistry，2018，127：336-342.
［23］Tyagi V，Parihar V，Malik G，et al. The DEAD-box RNA helicase eIF4A regulates plant development and interacts with the hnRNP LIF2L1 in Physcomitrella patens［J］. Molecular Genetics and Genomics，2020，295(2)：373-389.
［24］Kato A，Fujita S，Komeda Y. Identification and characterization of two genes encoding the eukaryotic initiation factor 4A in rice［J］. DNA Sequence，2001，12(5/6)：295-303.
［25］Xu R R，Zhang S Z，Huang J G，et al. Genome-wide comparative in silico analysis of the RNA helicase gene family in Zea mays and Glycine max：a comparison with Arabidopsis and Oryza sativa［J］. PLoS One，2013，8(11)：e78982.
［26］Gallie D R，Le H，Caldwell C，et al. The phosphorylation state of translation initiation factors is regulated developmentally and following heat shock in wheat［J］. The Journal of Biological Chemistry，1997，272(2)：1046-1053.
［27］Xu R R，Zhang S Z，Lu L T，et al. A genome-wide analysis of the RNA helicase gene family in Solanum lycopersicum［J］. Gene，2013，513(1)：128-140.
［28］许瑞瑞，高明刚，李明，等. 黄瓜RNA解旋酶基因家族的生物信息学鉴定和表达分析［J］. 上海交通大学学报，2018，36(3)：86-96.
［29］Tanner N K，Cordin O，Banroques J，et al. The Q motif a newly identified motif in DEAD box helicases may regulate ATP binding and hydrolysis［J］. Molecular Cell，2003，11(1)：127-138.
［30］Cordin O，Banroques J，Tanner N K，et al. The DEAD-box protein family of RNA helicases［J］. Gene，2006，367：17-37.
［31］Blum S，Schmid S R，Pause A，et al. ATP hydrolysis by initiation factor 4A is required for translation initiation in Saccharomyces cerevisiae［J］. Proceedings of the National Academy of Sciences of the United States of America，1992，89(16)：7664-7668.
［32］Pause A，Méthot N，Svitkin Y，et al. Dominant negative mutants of mammalian translation initiation factor eIF-4A define a critical role for eIF-4F in cap-dependent and cap-independent initiation of translation［J］. The EMBO Journal，1994，13(5)：1205-1215.
［33］Pause A，Sonenberg N. Mutational analysis of a DEAD box RNA helicase：the mammalian translation initiation factor eIF-4A［J］. The EMBO Journal，1992，11(7)：2643-2654.
［34］Rocak S，Linder P. DEAD-box proteins：the driving forces behind RNA metabolism［J］. Nature Reviews Molecular Cell Biology，2004，5(3)：232-241.
［35］Xu C，Zhang Z H，He J，et al. The DEAD-box helicase RCF1 plays roles in miRNA biogenesis and RNA splicing in Arabidopsis［J］. The Plant Journal，2023，116(1)：144-160.
［36］Ohtani M，Demura T，Sugiyama M. Arabidopsis root initiation defective1，a DEAH-box RNA helicase involved in pre-mRNA splicing，is essential for plant development［J］. The Plant Cell，2013，25(6)：2056-2069.
［37］Huang C K，Shen Y L，Huang L F，et al. The DEAD-box RNA helicase AtRH7/PRH75 participates in pre-rRNA processing，plant development and cold tolerance in Arabidopsis［J］. Plant and Cell Physiology，2016，57(1)：174-191.
［38］Li Q，Liu N K，Liu Q，et al. DEAD-box helicases modulate dicing body formation in Arabidopsis［J］. Science Advances，2021，7(18)：eabc6266.
［39］Yang Y Z，Ding S，Liu X Y，et al. The DEAD-box RNA helicase ZmRH48 is required for the splicing of multiple mitochondrial introns，mitochondrial complex biosynthesis，and seed development in maize［J］. Journal of Integrative Plant Biology，2023，65(11)：2456-2468.
［40］Lu C A，Huang C K，Huang W S，et al. DEAD-box RNA helicase 42 plays a critical role in pre-mRNA splicing under cold stress［J］. Plant Physiology，2020，182(1)：255-271.
［41］Liu Y N，Liu S J，Shi H Y，et al. The TSN1 binding protein RH31 is a component of stress granules and participates in regulation of salt-stress tolerance in Arabidopsis［J］. Frontiers in Plant Science，2021，12：804356.
［42］Zhang X D，Han Y X，Yang Z M，et al. DEAD-box RNA helicase 6 regulates drought and abscisic acid stress responses in rapeseed (Brassica napus)［J］. Gene，2023，886：147717.

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备注/Memo

备注/Memo:
收稿日期：2025-06-24
基金项目：山东省自然科学基金青年基金(编号：ZR2023QC089)；潍坊学院博士科研基金(编号：2023BS19)。
作者简介：李颖(1993—)，女，山东宁阳人，博士，讲师，主要从事园艺作物抗逆分子机制研究。E-mail：ying127567@163.com。
通信作者：许瑞瑞，博士，教授，硕士生导师，主要从事植物逆境分子生物学与基因表达调控等研究。E-mail：xuruirui2006@163.com。
更新日期/Last Update: 2026-04-20