[1]董延康,曹博,王兴丽,等.花苜蓿GASA基因家族鉴定及干旱胁迫下表达模式分析[J].江苏农业科学,2026,54(8):92-101.
 Dong Yankang,et al.Identification of GASA gene family in Medicago ruthenica and its expression pattern analysis under drought stress[J].Jiangsu Agricultural Sciences,2026,54(8):92-101.
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花苜蓿GASA基因家族鉴定及干旱胁迫下表达模式分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
92-101
栏目:
耐干旱基因鉴定
出版日期:
2026-04-20

文章信息/Info

Title:
Identification of GASA gene family in Medicago ruthenica and its expression pattern analysis under drought stress
作者:
董延康1曹博2王兴丽1王召明3李明雨1
1.草种创新与草地农业生态系统全国重点实验室/兰州大学草地农业科技学院,甘肃兰州 730020; 2.甘南藏族自治州农林牧草科学院,甘肃甘南 747099; 3.国家草业技术创新中心(筹),内蒙古呼和浩特 010051
Author(s):
Dong Yankanget al
关键词:
花苜蓿GASA基因干旱胁迫转录表达分析
Keywords:
-
分类号:
S188;S551+.701
DOI:
-
文献标志码:
A
摘要:
花苜蓿(Medicago ruthenica)是重要的豆科牧草,具有优良的抗逆性。GASA基因家族编码一类受赤霉素诱导、富含半胱氨酸的多肽,在植物生长发育、胁迫响应的过程中发挥着重要作用。本研究利用花苜蓿全基因组数据,系统鉴定其GASA基因家族成员,并分析其在干旱胁迫下的表达模式。共鉴定出21个花苜蓿GASA基因(MrGASA1~MrGASA21),理化性质分析结果显示,这些基因编码的蛋白质多为碱性蛋白,其中MrGASA6、MrGASA20具有较高的稳定性和亲水性。染色体定位结果表明,基因分布不均匀,并存在串联重复(如MrGASA1~MrGASA3、MrGASA9~ MrGASA11)和片段复制(如MrGASA4~MrGASA6)事件。通过系统发育分析将花苜蓿、拟南芥(Arabidopsis thaliana)、蒺藜苜蓿(Medicago truncatula)的GASA蛋白分为A、B、C 3类,其中A类成员最多。通过基因结构、保守基序分析揭示了家族成员的结构多样性,其中motif 1、motif 2高度保守,存在于所有成员中。启动子预测到大量与激素信号[赤霉素(GA)、水杨酸(SA)、茉莉酸甲酯(MeJA)]、非生物胁迫响应(干旱、低温)相关的顺式作用元件。转录组、qRT-PCR分析结果表明,MrGASA基因在干旱胁迫下呈现差异表达模式,其中MrGASA4、MrGASA5的相对表达量上调,而MrGASA9、MrGASA11、MrGASA15的相对表达量下调;MrGASA2、MrGASA4、MrGASA9在处理早期上调表达,MrGASA10下调表达。研究首次在花苜蓿中全面解析了GASA基因家族,揭示了其成员特征、进化关系及在干旱胁迫下的表达调控模式,可为解析其在花苜蓿抗旱过程中的分子网络提供依据。
Abstract:
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参考文献/References:

[1]Herzog M,Dorne A M,Grellet F. GASA a gibberellin-regulated gene family from Arabidopsis thaliana related to the tomato GAST1 gene[J]. Plant Molecular Biology,1995,27(4):743-752.
[2]Aubert D,Chevillard M,Dorne A M,et al. Expression patterns of GASA genes in Arabidopsis thaliana:the GASA4 gene is up-regulated by gibberellins in meristematic regions[J]. Plant Molecular Biology,1998,36(6):871-883.
[3]Wigoda N,Ben-Nissan G,Granot D,et al. The gibberellin-induced,cysteine-rich protein GIP2 from Petunia hybrida exhibits in planta antioxidant activity[J]. The Plant Journal,2006,48(5):796-805.
[4]Zhang S C,Wang X J. Expression pattern of GASA downstream genes of DELLA in Arabidopsis[J]. Chinese Science Bulletin,2008,53(24):3839-3846.
[5]Wang L,Wang Z,Xu Y Y,et al. OsGSR1 is involved in crosstalk between gibberellins and brassinosteroids in rice[J]. The Plant Journal,2009,57(3):498-510.
[6]Sun S L,Wang H X,Yu H M,et al. GASA14 regulates leaf expansion and abiotic stress resistance by modulating reactive oxygen species accumulation[J]. Journal of Experimental Botany,2013,64(6):1637-1647.
[7]Marshall E,Costa L M,Gutierrez-Marcos J. Cysteine-rich peptides (CRPs) mediate diverse aspects of cell-cell communication in plant reproduction and development[J]. Journal of Experimental Botany,2011,62(5):1677-1686.
[8]Matsubayashi Y. Posttranslationally modified small-peptide signals in plants[J]. Annual Review of Plant Biology,2014,65:385-413.
[9]Liu X,Zhang H P,Jiao H J,et al. Expansion and evolutionary patterns of cysteine-rich peptides in plants[J]. BMC Genomics,2017,18(1):610.
[10]Olsson V,Joos L,Zhu S S,et al. Look closely,the beautiful may be small:precursor-derived peptides in plants[J]. Annual Review of Plant Biology,2019,70:153-186.
[11]Kereszt A,Mergaert P,Montiel J,et al. Impact of plant peptides on symbiotic nodule development and functioning[J]. Frontiers in Plant Science,2018,9:1026.
[12]Uchi N,Fukudome M,Nozaki N,et al. Antimicrobial activities of cysteine-rich peptides specific to bacteriocytes of the pea aphid Acyrthosiphon pisum[J]. Microbes and Environments,2019,34(2):155-160.
[13]Zhong S,Liu M L,Wang Z J,et al. Cysteine-rich peptides promote interspecific genetic isolation in Arabidopsis[J]. Science,2019,364(6443):eaau9564.
[14]Nahirak V,Almasia N I,Hopp H E,et al. Snakin/GASA proteins:involvement in hormone crosstalk and redox homeostasis[J]. Plant Signaling & Behavior,2012,7(8):1004-1008.
[15]Nahirak V,Almasia N I,Fernandez P V,et al. Potato snakin-1 gene silencing affects cell division,primary metabolism,and cell wall composition[J]. Plant Physiology,2012,158(1):252-263.
[16]Nahirak V,Rivarola M,Gonzalez de Urreta M,et al. Genome-wide analysis of the snakin/GASA gene family in Solanum Tuberosum cv.Kennebec[J]. American Journal of Potato Research,2016,93(2):172-188.
[17]Fan S,Zhang D,Zhang L Z,et al. Comprehensive analysis of GASA family members in the Malus domestica genome:identification,characterization,and their expressions in response to apple flower induction[J]. BMC Genomics,2017,18(1):827.
[18]Kumar A,Singh A,Kumar P,et al. Giberellic acid-stimulated transcript proteins evolved through successive conjugation of novel motifs and their subfunctionalization[J]. Plant Physiology,2019,180(2):998-1012.
[19]Bindschedler L V,Whitelegge J P,Millar D J,et al. A two component chitin-binding protein from French bean-association of a proline-rich protein with a cysteine-rich polypeptide[J]. FEBS Letters,2006,580(6):1541-1546.
[20]Li K L,Bai X,Li Y,et al. GsGASA1 mediated root growth inhibition in response to chronic cold stress is marked by the accumulation of DELLAs[J]. Journal of Plant Physiology,2011,168(18):2153-2160.
[21]Ko C B,Woo Y M,Lee D J,et al. Enhanced tolerance to heat stress in transgenic plants expressing the GASA4 gene[J]. Plant Physiology and Biochemistry,2007,45(9):722-728.
[22]Zhang S C,Wang X J. Overexpression of GASA5 increases the sensitivity of Arabidopsis to heat stress[J]. Journal of Plant Physiology,2011,168(17):2093-2101.
[23]Alonso-Ramírez A,Rodríguez D,Reyes D,et al. Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds[J]. Plant Physiology,2009,150(3):1335-1344.
[24]Rubinovich L,Weiss D. The Arabidopsis cysteine-rich protein GASA4 promotes GA responses and exhibits redox activity in bacteria and in planta:GASA4 promotes GA responses[J]. The Plant Journal,2010,64(6):1018-1027.
[25]Zhong C M,Xu H,Ye S T,et al. Gibberellic acid-stimulated arabidopsis6 serves as an integrator of gibberellin,abscisic acid,and glucose signaling during seed germination in Arabidopsis[J]. Plant Physiology,2015,169(3):2288-2303.
[26]Roxrud I,Lid S E,Fletcher J C,et al. GASA4,one of the 14-member Arabidopsis GASA family of small polypeptides,regulates flowering and seed development[J]. Plant and Cell Physiology,2007,48(3):471-483.
[27]Shi L,Gast R T,Gopalraj M,et al. Characterization of a shoot-specific,GA3 and ABA-regulated gene from tomato[J]. The Plant Journal,1992,2(2):153-159.
[28]Taylor B H,Scheuring C F. A molecular marker for lateral root initiation:the RSI-1 gene of tomato (Lycopersicon esculentum Mill) is activated in early lateral root primordia[J]. Molecular and General Genetics,1994,243(2):148-157.
[29]Kotilainen M,Helariutta Y,Mehto M,et al. GEG participates in the regulation of cell and organ shape during corolla and carpel development in Gerbera hybrida[J]. The Plant Cell,1999,11(6):1093.
[30]Zhang S C,Yang C W,Peng J Z,et al. GASA5,a regulator of flowering time and stem growth in Arabidopsis thaliana[J]. Plant Molecular Biology,2009,69(6):745-759.
[31]Cheng X R,Wang S X,Xu D M,et al. Identification and analysis of the GASR gene family in common wheat (Triticum aestivum L.) and characterization of TaGASR34,a gene associated with seed dormancy and germination[J]. Frontiers in Genetics,2019,10:980.
[32]冯冠楠,安聪,丁鋆嘉,等. 狗尾草GASA基因家族鉴定及其在不同逆境下表达模式分析[J]. 草地学报,2022,30(6):1379-1387.
[33]谭政委,郭水柱,苏小雨,等. 全基因组水平金银花TCP基因家族的鉴定及表达模式分析[J]. 中国中药杂志,2024,55(5):1665-1676.
[34]Cremer T,Cremer M,Dietzel S,et al. Chromosome territories-a functional nuclear landscape[J]. Current Opinion in Cell Biology,2006,18(3):307-316.
[35]Hong X,Scofield D G,Lynch M. Intron size,abundance,and distribution within untranslated regions of genes[J]. Molecular Biology and Evolution,2006,23(12):2392-2404.
[36]Maniatis T,Tasic B. Alternative pre-mRNA splicing and proteome expansion in metazoans[J]. Nature,2002,418(6894):236-243.
[37]Ben-Nissan G,Lee J Y,Borohov A,et al. GIP,a Petunia hybrida GA-induced cysteine-rich protein:a possible role in shoot elongation and transition to flowering[J]. The Plant Journal,2004,37(2):229-238.
[38]Ben-Nissan G,Weiss D. The Petunia homologue of tomato gast1:transcript accumulation coincides with gibberellin-induced corolla cell elongation[J]. Plant Molecular Biology,1996,32(6):1067-1074.
[39]Reymond P,Farmer E E. Jasmonate and salicylate as global signals for defense gene expression[J]. Current Opinion in Plant Biology,1998,1(5):404-411.
[40]陈亚州. 拟南芥芥子油苷积累对外源茉莉酸甲酯的响应[D]. 哈尔滨:东北林业大学,2008.

相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2026-01-08
基金项目:国家自然科学基金青年科学基金(编号:32503265);中国博士后科学基金(编号:2025M770259)。
作者简介:董延康(1999—),男,甘肃秦安人,硕士研究生,主要从事牧草种质资源与分子育种研究,E-mail:1727171270@qq.com;共同第一作者:曹博(1988—),男,甘肃兰州人,畜牧师,主要从事牧草种植和畜牧养殖研究,E-mail:395183122@qq.com。
通信作者:李明雨,博士,主要从事牧草种质资源与分子育种研究。E-mail:limy19@lzu.edu.cn。
更新日期/Last Update: 2026-04-20