[1]董乐,杨涵,周丹宁,等.FoEG1对盐旱胁迫下红花种子萌发及幼苗生长的影响[J].江苏农业科学,2026,54(8):286-292.
 Dong Le,et al.Effects of FoEG1 on seed germination and seedling growth of safflower under salt and drought stress[J].Jiangsu Agricultural Sciences,2026,54(8):286-292.
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FoEG1对盐旱胁迫下红花种子萌发及幼苗生长的影响()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第8期
页码:
286-292
栏目:
干旱胁迫调控
出版日期:
2026-04-20

文章信息/Info

Title:
Effects of FoEG1 on seed germination and seedling growth of safflower under salt and drought stress
作者:
董乐1杨涵1周丹宁1田静蕊1张文龙3朱畇昊12
1.河南中医药大学药学院,河南郑州 450046; 2.呼吸疾病诊疗与新药研发河南省协同创新中心,河南郑州 450046; 3.遂平侨基肥业有限公司,河南驻马店 463100
Author(s):
Dong Leet al
关键词:
激活蛋白红花盐胁迫干旱胁迫基因表达种子萌发幼苗
Keywords:
-
分类号:
S567.21+9.01;Q945.78
DOI:
-
文献标志码:
A
摘要:
为探究尖孢镰刀菌源激活蛋白FoEG1对盐胁迫与干旱胁迫下红花种子萌发、幼苗生长及免疫相关基因表达的调控作用,阐明其增强植物抗逆性的机制,通过原核表达并纯化激活蛋白FoEG1,使用不同浓度(0.1、1.0、10.0 μg/mL)FoEG1处理红花种子,以Tris-HCl缓冲液为对照(CK),测定种子发芽指标和幼苗生长指标,并利用 qRT-PCR 技术分析胁迫响应相关基因的表达水平。结果显示,1.0 μg/mL FoEG1处理显著增强红花耐逆性,在盐胁迫下,该处理红花株高、茎粗、根长分别较对照提高23.67%、30.00%、81.83%,根干重为对照的3倍;10.0 μg/mL FoEG1处理的发芽率达86.67%。干旱胁迫下,1.0 μg/mL FoEG1处理的红花根长、根鲜重、根干重、茎粗分别较对照增加59.54%、67.35%、2500%、31.25%,发芽率达93.33%。基因表达分析结果显示,CtNPR和CtEDS1的表达量在2种胁迫下均显著上调。结果说明,FoEG1通过剂量依赖方式有效缓解盐旱胁迫对红花生长的抑制作用,最适浓度为1.0 μg/mL;通过促进根系发育、生物量积累以及激活水杨酸(SA)通路关键基因(NPR与EDS1)的表达,增强红花的抗逆性,其中NPR介导的SA通路在响应盐胁迫中起主导作用,而EDS1则在干旱胁迫响应中扮演核心调控角色。结果可为利用真菌源激活蛋白增强作物抗逆性提供理论基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期:2025-11-11
基金项目:河南省自然科学基金(编号:252300420130);国家自然科学基金(编号:81603232)。
作者简介:董乐(2001—),女,河南周口人,硕士研究生,研究方向为药用植物分子生物。E-mail:15890596169@163.com。
通信作者:朱畇昊,副教授,硕士生导师,主要从事药用植物分子生物研究。E-mail:guxinhan123@163.com。
更新日期/Last Update: 2026-04-20