|本期目录/Table of Contents|

[1]丁丽军,陈长春,夏前贤.环病毒Ⅱ型Cap蛋白单克隆抗体的制备及初步应用[J].江苏农业科学,2013,41(11):44-46.
 Ding Lijun,et al.Preparation and preliminary application of monoclonal antibodies against capsid protein Porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2013,41(11):44-46.
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环病毒Ⅱ型Cap蛋白单克隆抗体的制备及初步应用(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年11期
页码:
44-46
栏目:
生物技术
出版日期:
2013-11-25

文章信息/Info

Title:
Preparation and preliminary application of monoclonal antibodies against capsid protein Porcine circovirus type 2
作者:
丁丽军 陈长春 夏前贤
江苏农牧科技职业学院,江苏泰州 225300
Author(s):
Ding Lijunet al
关键词:
猪圆环病毒Ⅱ型Cap蛋白单克隆抗体
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
Q785
摘要:
利用PCV2病毒免疫8周龄BALB/c雌性小鼠,取其脾细胞与SP2/0骨髓瘤细胞融合,以原核表达的PCV2病毒Cap蛋白作为检测抗原进行ELISA检测,获得了1株PCV2单抗,命名为2E9。研究证实,用PCV2全病毒免疫小鼠,能获得特异性针对PCV2病毒的单克隆抗体,为建立特异性强、敏感性高、简便快速的PCV2检测方法打下了基础。
Abstract:
-

参考文献/References:

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[2]Allan G M,McNeilly F,Meehan B M,et al. Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain,Denmark and Northern Ireland[J]. Veterinary Microbiology,1999,66(2):115-123.
[3]Hamel A L,Lin L L,Sachvie C,et al. PCR detection and characterization of type-2 Porcine circovirus[J]. Canadian Journal of Veterinary Research,2000,64(1):44-52.
[4]Kennedy S,Segalés J,Rovira A,et al. Absence of evidence of Porcine circovirus infection in piglets with congenital tremors[J]. Journal of Veterinary Diagnostic Investigation,2003,15(2):151-156.
[5]Meehan B M,McNcilly F,McNair I. Isolation and chanracterization of Porcine circovirus 2 from cases of sow abortion and porcine dermatitis and nephropathy syndrome[J]. Arch Virol,2001,146(4):835-842.
[6]Mankertz A,Hillenbrand B. Analysis of transcription of Porcine circovirus type 1[J]. J Gen Virol,2002,83(11):2743-2751.
[7]Nawagitgul P,Morozov I,Bolin S R,et al. Open reading frame 2 of Porcine circovirus type 2 encodes a major capsid protein[J]. Journal of General Virology,2000,81(9):2281-2287.
[8]Pogranichnyy R M,Yoon K J,Harms P A,et al. Characterization of immune response of young pigs to Porcine circovirus type 2 infection[J]. Viral Immunology,2000,13(2):143-153.
[9]Liu Q,Tikoo S K,Babiuk L A. Nuclear localization of the ORF2 protein encoded by Porcine circovirus type 2[J]. Virology,2001,285(1):91-99.
[10]Lekcharoensuk P,Morozov I,Paul P S,et al. Epitope mapping of the major capsid protein of type 2 Porcine circovirus (PCV2)by using chimeric PCV1 and PCV2[J]. Journal of Virology,2004,78(15):8135-8145.
[11]Grierson S S,King D P,Wellenberg G J,et al. Genome sequence analysis of 10 Dutch Porcine circovirus type 2(PCV-2) isolates from a PMWS case-control study[J]. Research in Veterinary Science,2004,77(3):265-268.
[12]Segalés J,Olvera A,Grau-Roma L,et al. PCV-2 genotype definition and nomenclature[J]. Vet Rec,2008,162(26):867-868.

相似文献/References:

[1]陶宏卫,郭广富,曹军平,等.猪圆环病毒Ⅱ型盐城株的全基因组克隆与序列分析[J].江苏农业科学,2018,46(16):39.
 Tao Hongwei,et al.Cloning and sequence analysis of Yancheng isolates of porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2018,46(11):39.
[2]王琳琳,陈长春,徐婷婷,等.猪圆环病毒 Ⅱ 型Cap蛋白单克隆抗体的制备及初步应用[J].江苏农业科学,2013,41(07):30.
 Wang Linlin,et al.Preparation and preliminary application of monoclonal antibodies against capsid protein of porcine circovirus type 2[J].Jiangsu Agricultural Sciences,2013,41(11):30.

备注/Memo

备注/Memo:
收稿日期:2013-03-29
作者简介:丁丽军(1980—),男,江苏南通人,硕士,讲师,研究方向为兽医临床。E-mail:641042228@qq.com。
更新日期/Last Update: 2013-11-25