|本期目录/Table of Contents|

[1]梁文杰.梨S22-RNase基因全长cDNA克隆及序列分析[J].江苏农业科学,2013,41(12):34-37.
　Liang Wenjie.Full-length cDNA cloning and sequence analysis of S22-RNase gene from pears[J].Jiangsu Agricultural Sciences,2013,41(12):34-37.
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梨S22-RNase基因全长cDNA克隆及序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年12期
页码:
34-37
栏目:
生物技术
出版日期:
2013-12-25

文章信息/Info

Title:
Full-length cDNA cloning and sequence analysis of S22-RNase gene from pears
作者:
梁文杰1; 2
1.中南林业科技大学经济林育种与栽培国家林业局重点实验室，湖南长沙 410004； 2.温州科技职业学院，浙江温州 325006
Author(s):
Liang Wenjie
关键词:
砂梨; 自交不亲和性; S基因; 克隆; 序列分析
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
S661.201
摘要:
以砂梨品种“宝珠”雌蕊为材料提取RNA，通过采用RACE技术，获得了1个840 bp梨S基因的cDNA目的片段，经克隆后测序，证实该片段是梨S22-RNase的全长cDNA序列；分析表明，该基因的编码框由681个碱基组成，共编码227个氨基酸，与已克隆的梨S-RNase基因的cDNA序列的相似性为60%～90%。
Abstract:
-

参考文献/References:

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［9］Hoy-Taek K，Yutaka H，Hyun-jung K，et al. The presence of a new S-RNase allele(S10)in Asian pear(Pyrus pyrifolia (Burm；Nakai))［J］. Genetic Resources and Crop Evolution，2006，53(7)：1375-1383.
［10］Raspe O，Kohn J R. Population structure at the S-locus of Sorbus aucuparia L.(Rosaceae：Maloideae)［J］. Molecular Ecology，2007，16(6)：1315-1325.
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［12］Sassa H，Nishio T，Kowyama Y，et al. Self-incompatibility(S)alleles of the rosaceae encode members of a distinct class of the T2/S ribonuclease superfamily［J］. Molecular & General Genetics，1996，250(5)：547-557.

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备注/Memo

备注/Memo:
收稿日期：2013-05-09
基金项目：国家林业局重点项目(编号：2006-12)。
作者简介：梁文杰(1981—)，男，硕士，讲师，研究方向为林业生物技术和林业碳汇。E-mail：lwj60000@126.com。
更新日期/Last Update: 2013-12-25