|本期目录/Table of Contents|

[1]张丽霞,贾海燕.一种简便高效大肠杆菌感受态细胞制备方法[J].江苏农业科学,2013,41(12):41-42.
 Zhang Lixia,et al.A simple and efficient preparation method of Escherichia coli competent cells[J].Jiangsu Agricultural Sciences,2013,41(12):41-42.
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一种简便高效大肠杆菌感受态细胞制备方法(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第41卷
期数:
2013年12期
页码:
41-42
栏目:
生物技术
出版日期:
2013-12-25

文章信息/Info

Title:
A simple and efficient preparation method of Escherichia coli competent cells
作者:
张丽霞 贾海燕
南京农业大学应用植物基因组学实验室/作物遗传与种质创新国家重点实验室,江苏南京 210095
Author(s):
Zhang Lixiaet al
关键词:
大肠杆菌感受态细胞克隆
Keywords:
-
分类号:
A
DOI:
-
文献标志码:
Q93-33
摘要:
对大肠杆菌感受态细胞制备和保存条件研究进行总结,在结合多年实际操作经验的基础上,把实际操作中需注意的事项进行详细归纳总结,可以为进行基因克隆的科研人员提供帮助。
Abstract:
-

参考文献/References:

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[3]Li W,Xie H,Xie Z,et al. Exploring the mechanism of competence development in Escherichia coli using quantum dots as fluorescent probes[J]. Journal of Biochemical and Biophysical Methods,2004,58(1):59-66.
[4]Theodorou M C,Tiligada E,Kyriakidis D A. Extracellular Ca2+transients affect poly-(R)-3-hydroxybutyrate regulation by the AtoS-AtoC system in Escherichia coli[J]. Biochemical Journal,2009,417(3):667-672.
[5]Sambrook J,Russell D W. Molecular cloning:a laboratory manual[M]. New York:Cold Spring Harbor Laboratory Press,2011.
[6]梅运军,陈向东,谢志雄,等. Ca2+对诱导大肠杆菌摄取外源DNA的影响[J]. 武汉大学学报:理学版,2012(4):354-358.
[7]王友如. CaCl2浓度对感受态细胞转化效率的影响[J]. 湖北师范学院学报:自然科学版,2006,26(3):30-32.
[8]张岚岚,徐春燕,徐昌杰. 大肠杆菌感受态细胞转化能力的影响因素[J]. 细胞生物学杂志,2004,26(4):429-432.
[9]谢翎,陈红梅,尹翀,等. 大肠杆菌DH5α菌株感受态制备即转化率变化研究[J]. 生物学杂志,2011,28(4):4-6.
[10]唐颜苹,王小媚,何薇,等. 大肠杆菌感受态细胞保存条件的研究[J]. 华中农业大学学报,2008,27(6):745-748.

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备注/Memo

备注/Memo:
收稿日期:2013-03-25
作者简介:张丽霞(1977—),女,山西汾阳人,硕士,助理研究员,主要从事实验室管理、作物遗传育种研究。E-mail:amyzlx@njau.edu.cn。
更新日期/Last Update: 2013-12-25