|本期目录/Table of Contents|

[1]贺江,刘贤金.甲氧基有机磷农药广谱特异性多克隆抗体的亲和纯化[J].江苏农业科学,2014,42(04):33-35.
　He Jiang,et al.Preparation of affinity column for purification of broad-spectrum polyclonal antibody against O，O-dimethyl organophosphorus pesticides[J].Jiangsu Agricultural Sciences,2014,42(04):33-35.
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甲氧基有机磷农药广谱特异性多克隆抗体的亲和纯化(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年04期
页码:
33-35
栏目:
生物技术
出版日期:
2014-04-25

文章信息/Info

Title:
Preparation of affinity column for purification of broad-spectrum polyclonal antibody against O，O-dimethyl organophosphorus pesticides
作者:
贺江1; 2;  刘贤金2
1.湖南文理学院生命科学学院，湖南常德 415000； 2.江苏省农业科学院食品质量安全检测研究所，江苏南京 210014
Author(s):
He Jiang; et al
关键词:
亲和纯化; 甲氧基有机磷农药; 广谱特异性抗体; 免疫分析
Keywords:
-
分类号:
TQ450.7
DOI:
-
文献标志码:
A
摘要:
采用碳二亚胺法将甲氧基有机磷农药通用半抗原与琼脂糖凝胶进行链接，并利用链接产物对相应的多克隆抗血清进行纯化。经鉴定，偶联反应正常，纯化效果良好且产率约为78.5%；纯化后抗体能够保持对5种农药对象的识别活性，且具有更高的亲和活性。应用纯化后的抗体能够建立起更灵敏的免疫检测方法。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-08-20
基金项目：国家自然科学基金(编号：30871658)。
作者简介：贺江(1983—)，男，江西萍乡人，博士，讲师，主要从事食品安全与食品生物技术研究。E-mail：hejiang1119@163.com。
更新日期/Last Update: 2014-04-25