|本期目录/Table of Contents|

[1]荆彦平,刘大同,郝亚芳,等.胚乳淀粉体分离的简易方法[J].江苏农业科学,2014,42(04):73-74.
 Jing Yanping,et al.A convenient method to isolate amyloplast from endosperm[J].Jiangsu Agricultural Sciences,2014,42(04):73-74.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年04期
页码:
73-74
栏目:
育种栽培与生理生化
出版日期:
2014-04-25

文章信息/Info

Title:
A convenient method to isolate amyloplast from endosperm
作者:
荆彦平 刘大同 郝亚芳 王萍 李金萍 顾蕴洁 王忠
扬州大学江苏省作物遗传生理重点实验室/农业部长江中下游作物生理生态与栽培重点开放实验室,江苏扬州 225009
Author(s):
Jing Yanpinget al
关键词:
小麦玉米胚乳淀粉体低速离心
Keywords:
-
分类号:
S512.101
DOI:
-
文献标志码:
A
摘要:
以小麦、玉米为材料分离胚乳淀粉体,采用切割胚乳、低速离心法从胚乳中分离出淀粉体,利用0.5% I2-KI溶液对淀粉体进行染色,结果表明,小麦胚乳淀粉体呈近圆球形或椭球形,玉米胚乳淀粉体呈圆球形。玉米胚乳淀粉体形状较规则,淀粉体长、短轴差异较小,圆度较高。小麦胚乳淀粉体形状变化比较大,淀粉体长、短轴值差异较大,圆度较低。
Abstract:
-

参考文献/References:

[1]Sabelli P A,Larkins B A. The development of endosperm in grasses[J]. Plant Physiology,2009,149:14-26.
[2]蔡瑞国,尹燕枰,赵发茂,等. 强筋小麦胚乳淀粉粒度分布特征及其对弱光的响应[J]. 中国农业科学,2008,41(5):1308-1316.
[3]Echeverria E,Boyer C,Liu K C,et al. Isolation of amyloplasts from developing maize endosperm[J]. Plant Physiology,1985,77(3):513-519.
[4]Tetlow I J,Blissett K J,Emes M J. A rapid method for the isolation of purified amyloplasts from wheat endosperm[J]. Planta,1993,189(4):597-600.
[5]孔祥生,易现峰. 植物生理学实验技术[M]. 北京:中国农业出版社,2008:83-84.

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备注/Memo

备注/Memo:
收稿日期:2013-08-07
基金项目:国家自然科学基金(编号:3107134、 31270228); 高等学校博士学科点专项科研基金(编号:2009320004)。
作者简介:荆彦平(1989—),男,硕士研究生,从事谷物胚乳发育研究。E-mail: jingyanping1989@163.com。
通信作者:王忠,教授,从事植物生理学研究。E-mail: wangzhong@yzu.edu.cn。
更新日期/Last Update: 2014-04-25