|本期目录/Table of Contents|

[1]谢莹,刘阳,潘素丽,等.加工番茄无离层突变及离区JOINTLESS基因序列分析[J].江苏农业科学,2014,42(11):30-33.
 Xie Ying,et al(0).JOINTLESS gene sequence analysis of no separation mutation and abscission zone of processing tomato[J].Jiangsu Agricultural Sciences,2014,42(11):30-33.
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加工番茄无离层突变及离区JOINTLESS基因序列分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年11期
页码:
30-33
栏目:
生物技术
出版日期:
2014-11-25

文章信息/Info

Title:
JOINTLESS gene sequence analysis of no separation mutation and abscission zone of processing tomato
作者:
谢莹1 刘阳2 潘素丽1 陈宏宇2 赵婷婷1 王傲雪12
1.东北农业大学园艺学院,黑龙江哈尔滨 150030; 2.东北农业大学生命科学学院,黑龙江哈尔滨 150030
Author(s):
Xie Yinget al(30)
关键词:
加工番茄离区JOINTLESS基因突变序列分析
Keywords:
-
分类号:
S641.203
DOI:
-
文献标志码:
A
摘要:
以加工番茄有离层品种09872和无离层品种08003为试验材料,分别提取基因组DNA及有离层品种09872的花梗离区总RNA,设计引物扩增并测序,得到JOINTLESS基因序列(1 286 bp)、jointless(j)突变序列(347 bp)及JOINTLESS基因表达序列(797 bp)。采用生物学软件对序列进行生物信息学分析,结果表明,加工番茄的JOINTLESS基因编码序列与普通栽培番茄品种相比,有2个碱基发生突变;jointless(j)突变是由JOINTLESS基因第1个外显子的部分序列连同起始密码子上游共939 bp的碱基缺失所引起;JOINTLESS基因共编码265个氨基酸,预测所得的蛋白质整体表现为亲水性。经系统发育分析得出,加工番茄JOINTLESS基因序列与矮牵牛中该基因序列相似性最高,与葡萄物种的相似性最低。
Abstract:
-

参考文献/References:

[1]蒋先明. 蔬菜栽培学各论[M]. 北京:中国农业出版社,1999:17-21.
[2]Wing R A,Zhang H B,Tanksley S D. Map-based cloning in crop plants. Tomato as a model system:Ⅰ.Genetic and physical mapping of jointless[J]. Mol Gen Genet,1994,242(6):681-688.
[3]Zhang H B,Martin G B,Tanksley S D,et al. Map-based cloning in crop plants:tomato as a model system Ⅱ. Isolation and characterization of a set of overlapping yeast artificial chromosomes encompassing the jointless locus[J]. Mol Gen Genet,1994,244(6):613-621.
[4]Mao L,Begum D,Chuang H W,et al. Jointless is a MADS-box gene controlling tomato flower abscission zone development[J]. Nature,2000,406(6798):910-913.
[5]Leseberg C H,Eissler C L,Wang X,et al. Interaction study of MADS-domain proteins in tomato[J]. Journal of Experimental Botany,2008,59(8):2253-2265.

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备注/Memo

备注/Memo:
收稿日期:2014-01-02
基金项目:新世纪人才培养计划(编号:1251-NCET-004)。
作者简介:谢莹(1988—),女,硕士,从事园艺作物生物技术研究。E-mail:812489493@qq.com。
通信作者:王傲雪(1973—),男,博士,教授,博士生导师,从事园艺作物生物技术研究。E-mail:wangaoxue@yahoo.com。
更新日期/Last Update: 2014-11-25