|本期目录/Table of Contents|

[1]韩光杰,孙俊,李传明,等.苏云金芽孢杆菌原生质体的制备及大质粒转化[J].江苏农业科学,2014,42(11):37-40.
 Han Guangjie,et al(7).Preparation and transformation with large plasmid of Bacillus thuringiensis protoplasts[J].Jiangsu Agricultural Sciences,2014,42(11):37-40.
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苏云金芽孢杆菌原生质体的制备及大质粒转化(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年11期
页码:
37-40
栏目:
生物技术
出版日期:
2014-11-25

文章信息/Info

Title:
Preparation and transformation with large plasmid of Bacillus thuringiensis protoplasts
作者:
韩光杰 孙俊 李传明 刘琴 徐健
江苏里下河地区农业科学研究所,江苏扬州 225007
Author(s):
Han Guangjieet al(37)
关键词:
苏云金芽孢杆菌原生质体制备转化
Keywords:
-
分类号:
S482.3+9
DOI:
-
文献标志码:
A
摘要:
研究苏云金芽孢杆菌原生质体制备与再生的最佳条件,为进一步提高原生质体转化率打下基础。通过酶解法对苏云金芽孢杆菌库斯塔克亚种工业生产菌株(Bacillus thuringiensis ssp. kurstaki) 2671原生质体的制备及再生条件进行了研究,考察了菌体生长状态、溶菌酶浓度、处理时间等影响因素,采用PEG法对大质粒进行原生质转化。结果表明,对数生长前期的菌体(培养3 h),以SMML作渗透压稳定剂,溶菌酶浓度7 mg/mL,40 ℃下酶解40 min,原生质体生成量可达5.7×107个/mL,再生率可达14.1%。在此条件下,采用PEG法可将大质粒pLTV1-ep成功转化宿主菌,转化率为0.3 CFU/μg。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2014-07-28
基金项目:公益性行业(农业)科研专项(编号:201103021);国家自然科学基金(编号:301071740);江苏省农业科技自主创新资金[编号:CX(13)5069];江苏省农业科技支撑计划(编号:BE2011358);江苏扬州市农业科技攻关(编号:2012095)。
作者简介:韩光杰(1987—),男,安徽桐城人,硕士,研究实习员,主要从事农业害虫生物防治研究。E-mail:hanguangjie177@163.com。
通信作者:徐健,博士,研究员,主要从事农业害虫生物防治研究。Tel:(0514)87302019;E-mail:bio-xj@163.com。
更新日期/Last Update: 2014-11-25