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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第42卷

期数:

2014年12期

页码:

236-239

栏目:

畜牧兽医

出版日期:

2014-12-25

文章信息/Info

Title:

Correlation between cumulus cell diameter and oocyte quality of sheep

作者:

古丽米热·阿布都热依木¹; 2;  吴阳升²;  林嘉鹏²;  汪立芹²;  张利²; 3;  蒋香菊²; 3;  杨楠¹; 2;  唐淑红¹; 2;  黄俊成²

1.新疆农业大学，新疆乌鲁木齐 830000； 2.新疆畜牧科学院动物生物技术研究中心，新疆乌鲁木齐 830000；3.石河子大学动物科技学院，新疆石河子 832000

Author(s):

Gulimire Abudureyimu; et al

关键词:

绵羊; 卵丘细胞; 卵母细胞; RT-PCR

Keywords:

-

分类号:

S826.3

DOI:

-

文献标志码:

A

摘要:

利用RT-PCR方法，检测不同直径绵羊卵泡卵丘细胞中FSHR、EGFR、GHR、bFGF、CYP19A、AMH基因的mRNA表达规律及卵母细胞孤雌激活后胚胎发育的影响，结果表明，大、中、小卵泡卵丘细胞中都有FSHR、EGFR、GHR、bFGF、CYP19A、AMH的mRNA表达，不同直径卵泡卵丘细胞呈现递增趋势，大卵泡卵丘细胞与中小卵泡的卵丘细胞相对表达量差异显著(P<0.05)；AMH在直径>5.0 mm的卵泡卵丘细胞中有所下降。不同大小卵泡卵母细胞经体外培养，结果表明，各卵泡卵母细胞直径组间成熟率不显著，随卵泡直径的增大卵裂率增加，1.0～2.0 mm组与2.1～5.0 mm组卵裂率都显著低于>5 mm组 (P<0.05)，相互间无差异(P>0.05)；囊胚率也呈现逐渐增加的趋势，卵泡大于5.0 mm卵母细胞组极显著高于1.0～2.0 mm组(P<0.01)，2.1～5.0 mm组(P<0.05)。证实卵丘细胞在绵羊卵母细胞成熟和受精及随后的胚胎发育过程中起重要作用，卵泡直径对卵母细胞发育能力产生影响。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2014-01-16
基金项目：国家转基因重大专项(编号：2014ZX08008-003)；国家自然科学基金(编号：U1203381)；新疆维吾尔自治区科技计划(编号：201111113)；新疆维吾尔自治区科技支疆项目(编号：201291147)。
作者简介：古丽米热· 阿布都热依木(1985—)，女，新疆乌鲁木齐人，硕士，从事动物分子生物学研究。E-mail：rere127@163.com。
通信作者：黄俊成(1968—)，男，博士，研究员，从事家畜繁殖与胚胎生物

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更新日期/Last Update: 2014-12-25