|本期目录/Table of Contents|

[1]代艳艳,廖秋萍,贾桂珍,等.新疆南疆地区微小牛蜱Bm86基因克隆及鉴定[J].江苏农业科学,2015,43(01):24-26.
 Dai Yanyan,et al.Cloning and identification of Bm86 gene of Boophilus microplus in southern Xinjiang[J].Jiangsu Agricultural Sciences,2015,43(01):24-26.
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新疆南疆地区微小牛蜱Bm86基因克隆及鉴定(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年01期
页码:
24-26
栏目:
生物技术
出版日期:
2015-01-25

文章信息/Info

Title:
Cloning and identification of Bm86 gene of Boophilus microplus in southern Xinjiang
作者:
代艳艳 廖秋萍 贾桂珍 周桐 罗福江 周玉琴 王帅 赵丽 刘永宏
塔里木大学动物科学学院/新疆生产建设兵团塔里木畜牧科技重点实验室,新疆阿拉尔 843300
Author(s):
Dai Yanyanet al
关键词:
南疆微小牛蜱Bm86基因克隆
Keywords:
-
分类号:
S852.74+6
DOI:
-
文献标志码:
A
摘要:
为了克隆及鉴定新疆南疆地区微小牛蜱Bm86基因,参照GenBank登录的微小牛蜱Bm86基因序列,设计了可以扩增Bm86基因完整阅读框架的引物,对微小牛蜱进行总RNA提取、RT-PCR、克隆、测序、序列分析。结果表明,获得的Bm86基因长1 953 bp,与GenBank中登录号为M29321、HQ014398的微小牛蜱Bm86基因核苷酸同源性分别为99%、97%,且为一个完整的开放阅读框架。本研究获得的微小牛蜱Bm86基因,为获得基因工程疫苗的候选抗原及中国抗微小牛蜱疫苗制备奠定了基础。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-02-21
基金项目:新疆生产建设兵团博士资金(编号:2012BB023);塔里木大学大学生创新创业训练计划(编号:2013107570030)。
作者简介:代艳艳(1994—),女,河南鹿邑人,研究方向为动物传染病与免疫病理学。E-mail:2275781936@qq.com。
通信作者:赵丽,博士,副教授,主要从事动物疫病研究。E-mail:zhaolidky@126.com。
更新日期/Last Update: 2015-01-25