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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第43卷

期数:

2015年05期

页码:

167-170

栏目:

园艺与林学

出版日期:

2015-05-25

文章信息/Info

Title:

Analysis of relationship between jasmonic acid and Japanese apricot dormancy by liquid chromatography-tandem mass spectrometry

作者:

韩驰¹;  仲文君²;  高志红²;  霍希美²;  李百健³;  庄维兵²;  倪照君²

1.江苏省徐州市果树服务站，江苏徐州 221009； 2.南京农业大学园艺学院，江苏南京 210095； 3.南京傅家边科技园集团有限公司，江苏南京 211221

Author(s):

Han Chi; et al

关键词:

液相色谱-串联质谱; 果梅; 休眠; 茉莉酸

Keywords:

-

分类号:

O657.63

DOI:

-

文献标志码:

A

摘要:

建立了不同果梅(Prunus mume Sieb. et Zucc)品种4个休眠时期花芽的茉莉酸(jasmonic acid)含量的液相色谱-串联质谱(liquid chromatography-tandem triple quadrupole mass spectrometric，LC-MS/MS)测定方法。样品经过甲醇提取，Agela Cleanert SPE-NH₂固相萃取小柱净化。色谱分离采用C₁₈反相柱，以乙腈-水(水相含0.05%乙酸)为流动相梯度洗脱，质谱分析采用ESI离子源、二级串联质谱、负离子模式下进行检测。测定结果，方法标准曲线线性良好，茉莉酸r= 0.999 2，平均回收率范围为78.18%~97.49%，相对标准偏差(RSD，n=3)为1.25%~8.27%。该方法操作简便，灵敏度高，选择性好，回收率高，适合果梅花芽中茉莉酸的测定。结果表明，被测2个品种的果梅花芽中，休眠解除和生态休眠时期的茉莉酸含量高于内休眠和抑制性休眠时期，推测内源激素茉莉酸可能有抑制果梅休眠和促进花芽正常生长的作用。本试验对进一步探讨果梅休眠与内源激素茉莉酸的关系打下了一定的基础。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2014-06-05
基金项目：江苏省科技支撑计划(编号：BE2011458、BE2012361)；江苏省自然科学基金(编号：BK2011642)；江苏省“青蓝工程”。
作者简介：韩驰(1970—)，男，江苏徐州人，硕士，高级农艺师，主要从事果树技术推广。E-mail：xzsyyxh@163.com。
通信作者：高志红，博士，教授，主要从事果树分子生物技术研究。E-mail：gaozhihong@njau.edu.cn。

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更新日期/Last Update: 2015-05-25