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[1]汪义龙,夏莹萍,梁建生.水稻OsCATA基因相互作用蛋白的筛选和表达分析[J].江苏农业科学,2015,43(08):20-23.
　Wang Yilong,et al.Screening and gene expression of OsCATA-interactive protein in Oryza sativa[J].Jiangsu Agricultural Sciences,2015,43(08):20-23.
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水稻OsCATA基因相互作用蛋白的筛选和表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年08期
页码:
20-23
栏目:
生物技术
出版日期:
2015-08-25

文章信息/Info

Title:
Screening and gene expression of OsCATA-interactive protein in Oryza sativa
作者:
汪义龙;  夏莹萍;  梁建生
扬州大学生物科学与技术学院，江苏扬州 225009
Author(s):
Wang Yilong; et al
关键词:
水稻; OsCATA基因; 过氧化氢酶; 酵母双杂交; 表达分析
Keywords:
-
分类号:
Q786；S511.01
DOI:
-
文献标志码:
A
摘要:
根据水稻数据库中过氧化氢酶基因编码序列设计引物，克隆水稻OsCATA基因全长序列，构建无自激活活性的pGBKT7-OsCATA诱导重组质粒，采用酵母双杂交系统筛选水稻2周龄叶片 cDNA文库。结果表明，对84个阳性克隆测序，比对分析得到13种可能与OsCATA基因存在相互作用的蛋白质，这些蛋白与叶绿素合成前体、胁迫相应蛋白、核糖体蛋白、外膜蛋白、分泌蛋白、ATP酶和G蛋白β亚基等相关。不同时期过氧化氢酶基因表达分析显示，OsCATA基因在幼苗期和抽穗期表达较高，OsCATB基因在乳熟期和完熟期中表达较高，OsCATC基因在幼苗期和扬花期表达较高。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2014-09-21
基金项目：国家自然科学基金(编号：90917005/C020408)。
作者简介：汪义龙(1984—)，男，安徽霍山人，博士研究生，主要从事水稻光呼吸研究。E-mail: 95244465@qq.cn。
通信作者：梁建生，教授，博士生导师，研究方向为植物逆境生理与分子生物学、植物细胞信号转导、植物蛋白质组学、作物光合作用等。E-mail：jsliang@yzu.edu.cn。
更新日期/Last Update: 2015-08-25