|本期目录/Table of Contents|

[1]魏力.微拟球藻中潜在C4循环对不同抑制剂、光质的响应[J].江苏农业科学,2015,43(10):298-302.
 Wei Li.Effects of different chemical inhibitors and light quality on potential C4 cycle in Nannochloropsis oceanica IMET1[J].Jiangsu Agricultural Sciences,2015,43(10):298-302.
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微拟球藻中潜在C4循环对不同抑制剂、光质的响应(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年10期
页码:
298-302
栏目:
水产养殖·特种种养
出版日期:
2015-10-25

文章信息/Info

Title:
Effects of different chemical inhibitors and light quality on potential C4 cycle in Nannochloropsis oceanica IMET1
作者:
魏力12
1.中国科学院青岛生物能源与过程研究所单细胞研究中心,山东青岛 266101; 2.中国科学院大学,北京 100086
Author(s):
Wei Li
关键词:
微拟球藻C4循环抑制剂转录动态
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
为了探讨微拟球藻C3、C4循环在碳固定过程中的角色和功能,通过对其基因组、转录组进行解析,发现微拟球藻IMET1中存在编码磷酸烯醇式丙酮酸羧化酶、苹果酸酶、丙酮酸磷酸双激酶、磷酸烯醇式丙酮酸羧激酶等C4 途径关键酶的基因。分析了关键C4基因的进化,并通过实时荧光定量PCR法检测了微拟球藻在不同光质条件下C4基因(磷酸烯醇式丙酮酸羧化酶、磷酸烯醇式丙酮酸羧激酶)表达量的变化。此外通过使用不同的化学抑制剂检测这些潜在C4基因对其的响应。结果表明,IMET1中存在细菌型磷酸烯醇式丙酮酸羧化酶,在系统同发育关系上,磷酸烯醇式丙酮酸羧化酶、磷酸烯醇式丙酮酸羧激酶基因都与硅藻、褐藻进化关系很近。在白光、蓝光、红光等非生物胁迫条件下,磷酸烯醇式丙酮酸羧化酶、磷酸烯醇式丙酮酸羧激酶的基因表达在蓝光下短期内会显著升高,而后相对表达又下调。在某些胁迫条件下,磷酸烯醇式丙酮酸羧化酶、丙酮酸磷酸双激酶酶等活性的升高可能改变了微拟球藻IMET1碳代谢途径的走向,C4代谢途径功能加强。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2014-10-23
基金项目:国家自然科学基金国际合作项目(编号:31010103907)。
作者简介:魏力(1980—),男,内蒙古通辽人,博士,助理研究员,主要从事微藻碳固定机制的功能基因组学研究。Tel:(0532)80662658;E-mail:weili@qibebt.ac.cn。
更新日期/Last Update: 2015-10-25