|本期目录/Table of Contents|

[1]刘广志,陈炳佑,侍福梅.MAP18参与了脱落酸调控的拟南芥气孔关闭及根生长[J].江苏农业科学,2015,43(11):55-57.
　Liu Guangzhi,et al.MAP18 involved in stomatal closure and root growth of Arabidopsis thaliana regulated by abscisic acid[J].Jiangsu Agricultural Sciences,2015,43(11):55-57.
点击复制

MAP18参与了脱落酸调控的拟南芥气孔关闭及根生长(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年11期
页码:
55-57
栏目:
生物技术
出版日期:
2015-11-25

文章信息/Info

Title:
MAP18 involved in stomatal closure and root growth of Arabidopsis thaliana regulated by abscisic acid
作者:
刘广志;  陈炳佑;  侍福梅
聊城大学生命科学学院，山东聊城 252059
Author(s):
Liu Guangzhi; et al
关键词:
MAP18; 拟南芥; 脱落酸; 气孔; 幼根; 微管结合蛋白
Keywords:
-
分类号:
Q945
DOI:
-
文献标志码:
A
摘要:
以拟南芥野生型、GFP-α-tubulin-6转基因拟南芥及微管结合蛋白MAP18相关突变体为材料，利用药理学试验及激光扫描共聚焦显微技术研究了在脱落酸(ABA)诱导叶片气孔关闭过程中保卫细胞微管骨架的动态变化、根生长情况及其可能的调控因子。结果表明，微管结合蛋白MAP18过表达拟南芥OE的叶片气孔关闭现象敏感，MAP18 RNAi沉默株R18现象相对不敏感。外源ABA抑制拟南芥幼苗初生根的生长试验中，突变体OE根伸长减慢的现象显著，伸长速率由0.60 cm/d降低至0.12 cm/d，未处理时WT、map18的伸长速率都要低于OE的伸长速率，而用5 μmol/L ABA处理之后，WT、map18的伸长速率高于OE的伸长速率。在此基础上研究了参与其中的作用因子，推测MAP18通过影响微管的动态重组参与到ABA诱导拟南芥气孔关闭和根伸长的生理过程。
Abstract:
-

参考文献/References:

［1］Schroeder J I，Allen G J，Hugouvieux V，et al. Guard cell signal transduction［J］. Annual Review of Plant Biology，2001，52(1)： 627-658.
［2］戴艳红，郭栋梁，李玲. ABA抑制花生侧根发生［J］. 植物生理学报，2011，47(1)：75-79.
［3］Sharp R E，Poroyko V，Hejlek L G，et al. Root growth maintenance during water deficits：physiology to functional genomics［J］. Journal of Experimental Botany，2004，55(407)： 2343-2351.
［4］Staiger C J. Signaling to the action cytoskeleton in plants［J］. Annual Review of Plant Biology，2000，51(1)： 257-288.
［5］Wasteneys G O，Galway M E. Remodelling the cytoskeleton for growth and form： an overview with some new views［J］. Annual Review of Plant Biology，2003，54(1)： 691-722.
［6］Dzierzynska A. The role of cytoskeleton in stomata functioning［J］. Acta Physiologiae Plantarum，2006，28：59-79.
［7］Zhang W，Fan L M，Wu W H. Osmo-sensitive and stretch-activated calcium-permeable channels in Vicia faba guard cells are regulated by actin dynamics［J］. Plant Physiology，2007，143(3)：1140-1151.
［8］Wang X L，Gao X Q，Wang X C. Stochastic dynamics of actin filaments in guard cells regulating chloroplast localization during stomatal movement［J］. Plant Cell & Environment，2011，34(8)：1248-1257.
［9］Higaki T，Kutsuna N，Sano T，et al. Quantification and cluster analysis of actin cytoskeletal structures in plant cells：role of actin bundling in stomatal movement during diurnal cycles in Arabidopsis guard cells［J］. The Plant Journal，2010，61(1)：156-165.
［10］叶子，黄聪聪，于荣. 保卫细胞微管骨架参与蛋白丝氨酸/苏氨酸磷酸化调节的气孔运动［J］. 中国农业科学，2012，45(21)：4351-4360.
［11］张媛华. G蛋白在暗调控气孔运动中的作用［J］. 湖北农业科学，2013(4)：840-841，845.
［12］Wang X，Zhu L，Liu B Q，et al. Arabidopsis miceotubule-associated protein18 functions in directional cell growth by destabilizing cortical microtubules［J］. Plant Cell，2007，19(3)：877-889.
［13］Zhu L，Zhang Y，Kang E F，et al. MAP18 regulates the direction of pollen tube growth in arabidopsis by modulating F-Actin organization［J］. Plant Cell，2013，25(3)：851-867.
［14］林坤，周生波，王超，等. MAP18参与ABA调控拟南芥花粉生长的研究［J］. 安徽农业科学，2012，40(13)：7629-7630，7734.
［15］Yu R，Huang R F，Wang X C，et al. Microtubule dynamics are involved in stomatal movement of Vicia faba L.［J］. Protoplasma，2001，216(1/2)：113-118.
［16］Mathur J. Plant cytoskeleton：reinforcing lines of division in plant cells［J］. Current Biology，2004，14(7)：R287-R289.
［17］Yuan M，Shaw P J，Warn R M，et al. Dynamic reorientation of cortical microtubules，from transverse to longitudinal，in living plant-cells［J］. Proceedings of the National Academy of Sciences of the United States of America，1994，91(13)：6050-6053.
［18］Mathur J，Mathur N，Kernebeck B，et al. A novel localization pattern for an EB1-like protein links microtubule dynamics to endomembrane organization［J］. Current Biology，2003，13(22)：1991-1997.

相似文献/References:

[1]王宏归,黄晨,姜雅,等.CONSTANS LIKE 7参与调控拟南芥的向地性以及侧根、子叶的发育[J].江苏农业科学,2015,43(12):48.
　Wang Honggui,et al.Study on CONSTANS LIKE 7 involved in regulating gravitropism and development of side root and cotyledon in Arabidopsis[J].Jiangsu Agricultural Sciences,2015,43(11):48.
[2]李雪,邵铁梅,安胜军.1种简单方便的拟南芥发芽诱导新技术[J].江苏农业科学,2015,43(12):51.
　LI Xue,et al.A simple and convenient technology for bud induction of Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2015,43(11):51.
[3]王琳,孙庆玲,刘辉,等.拟南芥缺失突变体at14a的比较转录组分析[J].江苏农业科学,2016,44(04):70.
　Wang Lin,et al.Comparative transcriptional analysis of mutant at14a of Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2016,44(11):70.
[4]韩蕾,李俊林,苏彦华.拟南芥突变体kea的表型分析及对生长素的响应特征[J].江苏农业科学,2016,44(06):30.
　Han Lei,et al.Phenotypic analysis of arabidopsis mutant kea and its response to exogenous auxin[J].Jiangsu Agricultural Sciences,2016,44(11):30.
[5]奈婕菲,程玉祥.一个杨树GDSL基因组织表达的特性及其在拟南芥异源的表达[J].江苏农业科学,2014,42(03):16.
　Nai Jiefei,et al.Tissue expression of a poplar GDSL gene and its heterologous expression analysis in Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2014,42(11):16.
[6]郭瑾,薛永来,杜道林.植物激素调控拟南芥根系发育的研究进展[J].江苏农业科学,2014,42(05):7.
　Guo Jin,et al.Research progress of phytohormones regulating root system development of Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2014,42(11):7.
[7]姜上川,梅超,王小芳,等.PPR蛋白APPR6参与ABA调控拟南芥种子萌发与幼苗生长[J].江苏农业科学,2016,44(04):53.
　Jiang Shangchuan,et al.PPR protein APPR6 involved in ABA regulation of seed germination and seedling growth in Arabidopsis[J].Jiangsu Agricultural Sciences,2016,44(11):53.
[8]李静婷,赵旭耀,刘超凡,等.热胁迫对转TasHSP16.9拟南芥幼苗生长生理特性的影响[J].江苏农业科学,2016,44(10):113.
　Li Jingting,et al.Effects of heat stress on growth and physiological indices of TasHSP16.9 transgenic Arabidopsis thaliana seedlings[J].Jiangsu Agricultural Sciences,2016,44(11):113.
[9]郝东利,杨顺瑛,黄亚楠,等.拟南芥铵转运蛋白AtAMT1.3的电生理功能[J].江苏农业科学,2017,45(08):36.
　Hao Dongli,et al.Electrophysiological study on Arabidopsis ammonium transporter AtAMT1.3[J].Jiangsu Agricultural Sciences,2017,45(11):36.
[10]郭丽红,徐娅,郤秋霞,等.拟南芥热激因子AtHsfA1a在低温胁迫下对细胞程序性死亡中Caspase-3活性的影响[J].江苏农业科学,2017,45(21):24.
　Guo Lihong,et al.Effects of heat shock factor AtHsfA1a on Caspase-3 enzyme activity during programmed cell death under low temperature stress in Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2017,45(11):24.

备注/Memo

备注/Memo:
收稿日期：2014-12-23
基金项目：国家自然科学基金(编号：31240035)；山东省自然科学基金(编号：ZR2010CQ002)。
作者简介：刘广志(1989—)，男，山东聊城人，硕士研究生，研究方向为生物化学与分子生物学。 E-mail：liuguangzhi_568@163.com。
通信作者：侍福梅，博士，副教授，从事模式植物及重要农作物环境应答的信号转导研究。E-mail： shifumei@lcu.edu.cn。
更新日期/Last Update: 2015-11-25