|本期目录/Table of Contents|

[1]李静婷,王健胜,杨风岭.小麦MITE转座子对sHSP基因的表达调控研究[J].江苏农业科学,2015,43(12):29-32.
 Li Jingting,et al.Regulation of MITE on expression of sHSP gene in wheat[J].Jiangsu Agricultural Sciences,2015,43(12):29-32.
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小麦MITE转座子对sHSP基因的表达调控研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年第12期
页码:
29-32
栏目:
生物技术
出版日期:
2015-12-25

文章信息/Info

Title:
Regulation of MITE on expression of sHSP gene in wheat
作者:
李静婷1 王健胜2 杨风岭2
1.平顶山学院化学与环境工程学院,河南平顶山 467000;2.平顶山学院低山丘陵区生态修建重点实验室,河南平顶山 467000
Author(s):
Li Jingtinget al
关键词:
MITE转座子sHSP基因基因表达基因调控小麦植物双元表达载体构建
Keywords:
-
分类号:
Q786;S512.103
DOI:
-
文献标志码:
A
摘要:
MITE(miniature inverted-repeat transposable elements)是一类特殊的DNA介导的转座子,经常插入基因的编码区、内含子、启动子、非翻译区(UTR)而与基因紧密相关。探讨插入小麦16.9 ku sHSP基因(sHSP16.9)3′-UTR中MITE对基因表达调控的影响,实时定量结果显示:在高温、低温处理时,具有MITE插入的基因型中sHSP16.9基因转录水平较无MITE插入的基因型显著提高;构建2种不同的植物表达载体pCAMBIA Super-1300 + sHSP16.9、pCAMBIA Super-1300 + sHSP16.9+MITE转化拟南芥,半定量结果显示,sHSP16.9+MITE超表达的转基因拟南芥中sHSP16.9基因表达量较sHSP16.9超表达的转基因拟南芥中显著提高,推测sHSP16.9基因3′-UTR中 MITE插入增强该基因的转录水平。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2015-01-05
基金项目:河南省教育厅自然科学基础研究计划(编号:12B180020);平顶山学院高层次人才科研启动基金(编号:2011015G)。
作者简介:李静婷(1983—),女,内蒙古包头人,博士,讲师,主要从事小麦遗传育种学研究。E-mail:jingting_lee@163.com。
更新日期/Last Update: 2015-12-25