|本期目录/Table of Contents|

[1]王琳,孙庆玲,刘辉,等.拟南芥缺失突变体at14a的比较转录组分析[J].江苏农业科学,2016,44(04):70-74.
　Wang Lin,et al.Comparative transcriptional analysis of mutant at14a of Arabidopsis thaliana[J].Jiangsu Agricultural Sciences,2016,44(04):70-74.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年04期
页码:
70-74
栏目:
生物技术
出版日期:
2016-04-25

文章信息/Info

Title:
Comparative transcriptional analysis of mutant at14a of Arabidopsis thaliana
作者:
王琳;  孙庆玲;  刘辉;  梁建生
扬州大学生物科学与技术学院，江苏扬州 225009
Author(s):
Wang Lin; et al
关键词:
AT14A; 拟南芥; 缺失突变体; 基因芯片; 转录组学
Keywords:
-
分类号:
S188；Q786
DOI:
-
文献标志码:
A
摘要:
以at14a相关基因型(野生、缺失)拟南芥植株为试验材料，利用转录组学和生物信息学等方法，分析野生型(col)、缺失突变体(at14a)拟南芥差异基因表达状况，初步探讨拟南芥AT14A的功能。结果表明：at14a在拟南芥植株根、茎、叶和花中均有表达，尤其在叶片中的表达量较高；相较于野生型，缺失突变体at14a中有194个差异基因，其中上调基因122个，下调基因72个；通过GO富集分析发现，2种基因型植株差异基因主要参与了应答胁迫过程，这表明at14a可能通过调控AT5G39610、AT1G53240、AT4G32770、AT1G02920、AT1G10760这些逆境胁迫相关基因的表达来参与应答胁迫。差异分子主要定位于细胞核，具有绑定的分子功能。通过pathway分析发现，2个基因型植株差异基因主要参与了核糖体代谢、碳水化合物代谢、光合等代谢途径。研究结果为深入研究at14a作用的分子机理奠定了基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2016-01-27
基金项目：国家自然科学基金(编号：31271622)；扬州大学科技创新培育基金(编号：2015CXJ065)。
作者简介：王琳(1978一 )，女，江苏徐州人，博士研究生，讲师，主要从事植物生理和生化研究。E-mail：wanglin@yzu.edu.cn。
通信作者：梁建生，博士，教授，主要从事植物逆境生理与分子生物学研究。E-mail：jssliang@163.com。
更新日期/Last Update: 2016-04-25