|本期目录/Table of Contents|

[1]邹华锋,冯宇红,兰兆辉,等.低温处理对斑马鱼CNSS系统应激相关基因的影响[J].江苏农业科学,2016,44(08):43-46.
 Zou Huafeng,et al.Effect of low temperature on stress related gene expression in CNSS of zebrafish[J].Jiangsu Agricultural Sciences,2016,44(08):43-46.
点击复制

低温处理对斑马鱼CNSS系统应激相关基因的影响(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年08期
页码:
43-46
栏目:
生物技术
出版日期:
2016-08-25

文章信息/Info

Title:
Effect of low temperature on stress related gene expression in CNSS of zebrafish
作者:
邹华锋 冯宇红 兰兆辉 吕为群
上海海洋大学水产与生命学院,上海 201306
Author(s):
Zou Huafenget al
关键词:
斑马鱼尾部神经系统尾加压素基因表达低温
Keywords:
-
分类号:
Q344+.1
DOI:
-
文献标志码:
A
摘要:
选取斑马鱼在28、18 ℃条件下处理2周,对尾部神经系统CNSS组织的UⅠ、CRH、UⅡα、UⅡβM2基因表达以及斑马鱼的游泳运动行为进行了研究。经过寒冷应激后,试验组应激相关基因CRH、UⅡα、UⅡβM2表达与对照组相比均明显上升,但对UⅠ基因表达没有影响。对斑马鱼的运动轨迹进行分析表明,2个组的斑马鱼在快速游泳距离、慢速游泳距离、平均游泳速度方面均存在明显差异,低温处理组斑马鱼的慢速游泳时间明显高于对照组,平均游泳速度明显低于对照组。行为学数据和基因表达分析均表明,寒冷处理对斑马鱼造成了应激反应,低温条件下斑马鱼活动行为的减少可能是降低机体能量消耗的一种生存策略。
Abstract:
-

参考文献/References:

[1]Le M C,Troutaud D,Deschaux P. Differential effects of temperature on specific and nonspecific immune defences in fish[J]. The Journal of Experimental Biology,1998,201(Pt 2):165-168.
[2]邹曙明,沈俊宝,孙效文. 鱼类低温适应机制及抗寒育种[J]. 上海水产学报,1998,7(3):231-237.
[3]Donaldson M R,Cooke S J,Patterson D A,et al. Cold shock and fish[J]. Journal of Fish Biology,2008,73(7):1491-1530.
[4]Winter M J,Ashworth A,Bond H,et al. The caudal neurosecretory system:control and function of a novel neuroendocrine system in fish[J]. Biochemistry and Cell Biology,2000,78(3):193-203.
[5]吕为群,刘爽,钟英斌. 鱼类尾部神经分泌系统研究进展[J]. 上海海洋大学学报,2012,21(5):743-749.
[6]Bernier N J,Alderman S L,Bristow E N. Heads or tails?Stressor-specific expression of corticotropin-releasing factor and urotensinⅠ in the preoptic area and caudal neurosecretory system of rainbow trout[J]. Journal of Endocrinology,2008,196(3):637-648.
[7]Schaefer J,Ryan A. Developmental plasticity in the thermal tolerance of zebrafish Danio rerio[J]. Journal of Fish Biology,2006,69(3):722-734.
[8]Cortemeglia C,Beitinger T L. Temperature tolerances of wild-type and red transgenic zebra danios[J]. Transactions of the American Fisheries Society,2005,134(6):1431-1437.
[9]Uliano E,Cataldi M,Carella F,et al. Effects of acute changes in salinity and temperature on routine metabolism and nitrogen excretion in gambusia (Gambusia affinis) and zebrafish (Danio rerio)[J]. Comparative Biochemistry and Physiology. Part A,Molecular & Integrative Physiology,2010,157(3):283-290.
[10]Tseng Y C,Liu S T,Hu M Y,et al. Brain functioning under acute hypothermic stress supported by dynamic monocarboxylate utilization and transport in ectothermic fish[J]. Frontiers in Zoology,2014,11(1):53-58.
[11]Alderman S L,Bernier N J. Ontogeny of the corticotropin-releasing factor system in zebrafish[J]. General and Comparative Endocrinology,2009,164(1):61-69.
[12]Pepels P P,Van H H,Wendelaar B S E,et al. Corticotropin-releasing hormone in the teleost stress response:rapid appearance of the peptide in plasma of tilapia (Oreochromis mossambicus)[J]. The Journal of Endocrinology,2004,180(3):425-438.
[13]Wardle C S. Effects of temperature on the maximum swimming speed of fishes[M]//ALi M A.Environmental physiology of fishes.New York:Spinger,1980:519-531.
[14]Lu W,Greenwood M,Dow L,et al. Molecular characterization and expression of urotensin Ⅱ and its receptor in the flounder (Platichthys flesus):a hormone system supporting body fluid homeostasis in euryhaline fish[J]. Endocrinology,2006,147(8):3692-3708.
[15]Doyon C,Trudeau V L,Moon T W. Stress elevates corticotropin-releasing factor (CRF) and CRF-binding protein mRNA levels in rainbow trout (Oncorhynchus mykiss)[J]. The Journal of Endocrinology,2005,186(1):123-130.
[16]Hsu D T,Chen F L,Takahashi L K,et al. Rapid stress-induced elevations in corticotropin-releasing hormone mRNA in rat central amygdala nucleus and hypothalamic paraventricular nucleus:an in situ hybridization analysis[J]. Brain Research,1998,788(1/2):305-310.
[17]Lu W,Dow L,Gumusgoz S,et al. Coexpression of corticotropin-releasing hormone and urotensin I precursor genes in the caudal neurosecretory system of the euryhaline flounder (Platichthys flesus):a possible shared role in peripheral regulation[J]. Endocrinology,2004,145(12):5786-5797.
[18]Westring C G,Ando H,Kitahashi T,et al. Seasonal changes in CRF-I and urotensin Ⅰ transcript levels in masu salmon:correlation with cortisol secretion during spawning[J]. General and Comparative Endocrinology,2008,155(1):126-140.
[19]Craig P M,Al-Timimi H,Bernier N J. Differential increase in forebrain and caudal neurosecretory system corticotropin-releasing factor and urotensin Ⅰ gene expression associated with seawater transfer in rainbow trout[J]. Endocrinology,2005,146(9):3851-3860.
[20]竺晓鸣,杜冠华. 尾加压素Ⅱ生物学效应研究进展[J]. 中国药理学通报,2006,22(6):651-654.
[21]Larson B A,Madani Z. Increased urotensin Ⅰ and Ⅱ immunoreactivity in the urophysis of Gillichthys mirabilis transferred to low salinity water[J]. General and Comparative Endocrinology,1991,83(3):379-387.
[22]Chou M Y,Hsiao C D,Chen S C,et al. Effects of hypothermia on gene expression in zebrafish gills:upregulation in differentiation and function of ionocytes as compensatory responses[J]. The Journal of Experimental Biology,2008,211(Pt 19):3077-3084.
[23]Zhang W L,Yamada M,Gomeza J,et al. Multiple muscarinic acetylcholine receptor subtypes modulate striatal dopamine release,as studied with M-1-M-5 muscarinic receptor knock-out mice[J]. Journal of Neuroscience,2002,22(15):6347-6352.
[24]Perry E K,Smith C J,Court J A,et al. Cholinergic nicotinic and muscarinic receptors in dementia of Alzheimer,Parkinson and Lewy body types[J]. Journal of Neural Transmission:Parkinsons Disease and Dementia Section,1990,2(3):149-158.
[25]Hemrick L S K,Bymaster F P,Evans D C,et al. Muscarinic agonist-mediated increases in serum corticosterone levels are abolished in m(2) muscarinic acetylcholine receptor knockout mice[J]. The Journal of Pharmacology and Experimental Therapeutics,2002,303(1):99-103.

相似文献/References:

[1]郑伟,严继舟.斑马鱼组织石蜡切片质量的优化[J].江苏农业科学,2013,41(11):260.
 Zheng Wei,et al.Optimization of quality of Danio rerio tissue paraffin sections[J].Jiangsu Agricultural Sciences,2013,41(08):260.
[2]裘丽萍,范立民,刘琦,等.3种苯胺类对斑马鱼的急性毒性[J].江苏农业科学,2016,44(06):325.
 Qiu Liping,et al.Acute toxicity of three kinds of aniline on Brachydanio rerio[J].Jiangsu Agricultural Sciences,2016,44(08):325.
[3]赵月,曹志会,王冰,等.生化法处理后的链霉素废水出水对斑马鱼SOD活性和MDA含量的影响[J].江苏农业科学,2015,43(08):348.
 Zhao Yue,et al.Effects of streptomycin wastewater effluent after biochemical treatment on superoxide dismutase (SOD) activity and malondialdehyde (MDA) contents in muscle tissue of Danio rerio[J].Jiangsu Agricultural Sciences,2015,43(08):348.
[4]伍冰倩,刘妮妮,杨鑫豪,等.新型免疫分子TRIM23参与斑马鱼抗嗜水气单胞菌免疫应答[J].江苏农业科学,2019,47(04):153.
 Wu Bingqian,et al.A new immune molecule TRIM23 participating in immune response of zebrafish resisting Aeromonas hydrophila[J].Jiangsu Agricultural Sciences,2019,47(08):153.
[5]王黎曌,薛永来,高璐,等.纳米氧化铜对斑马鱼甲状腺系统的干扰效应[J].江苏农业科学,2019,47(14):202.
 Wang Lizhao,et al.Interference effects of CuO NPs on thyroid system in zebrafish[J].Jiangsu Agricultural Sciences,2019,47(08):202.
[6]吴若函,郭晓瑜,辛星,等.6种稻田除草剂对斑马鱼和大型溞的急性毒性[J].江苏农业科学,2021,49(19):223.
 Wu Ruohan,et al.Acute toxicity of six herbicides to Brachydanio rerio and Daphnia magna Straus in paddy fields[J].Jiangsu Agricultural Sciences,2021,49(08):223.

备注/Memo

备注/Memo:
收稿日期:2015-06-19
基金项目:上海海洋大学博士启动基金(编号:A-0209-13-0105384)。
作者简介:邹华锋(1976—),男,博士,讲师,主要从事鱼类应激生理学研究。Tel:(021)61900494;E-mail:hfzou@shou.edu.cn。
通信作者:吕为群,博士,教授,主要从事鱼类适应生理学和鱼类抗逆良种培育研究。E-mail:wqlv@shou.edu.cn。
更新日期/Last Update: 2016-08-25