|本期目录/Table of Contents|

[1]杜亚琳.黄瓜缺铜响应转录因子CsSPL7的克隆及表达分析[J].江苏农业科学,2016,44(12):88-91.
　Du Yalin.Cloning and expression analysis of copper deficiency response transcription factor CsSPL7 in cucumber[J].Jiangsu Agricultural Sciences,2016,44(12):88-91.
点击复制

黄瓜缺铜响应转录因子CsSPL7的克隆及表达分析(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第44卷
期数:
2016年12期
页码:
88-91
栏目:
生物技术
出版日期:
2016-12-25

文章信息/Info

Title:
Cloning and expression analysis of copper deficiency response transcription factor CsSPL7 in cucumber
作者:
杜亚琳
农业部东北地区园艺作物生物学与种质创制重点实验室/东北农业大学园艺学院，黑龙江哈尔滨 150030
Author(s):
Du Yalin
关键词:
CsSPL7基因; 黄瓜; 铜; QRT-PCR; 同源克隆
Keywords:
-
分类号:
S642.201
DOI:
-
文献标志码:
A
摘要:
结合黄瓜基因组数据和生物信息学分析，采用同源扩增技术从黄瓜津研4号叶片中获得1个受缺铜诱导表达的基因，命名为CsSPL7。该基因全长1 746 bp，编码582个氨基酸，与拟南芥SPL7基因序列相似性为43%。QRT-PCR表达分析表明，CsSPL7是1个受缺铜诱导表达的基因，在缺铜处理的黄瓜叶片中表达量最高，并且随着铜处理浓度的升高，其表达量逐渐降低。研究结果为下一步分析CsSPL7在黄瓜缺铜胁迫过程中的功能奠定了基础。
Abstract:
-

参考文献/References:

［1］Pilon M，Abdel-Ghany S E，Cohu C M，et al. Copper cofactor delivery in plant cells［J］. Current Opinion in Plant Biology，2006，9(3)：256-263.
［2］Burkhead J L，Reynolds K A G，Abdel-Ghany S E，et al. Copper homeostasis［J］. New Phytologist，2009，182(4)：799-816.
［3］Cohu C M，Pilon M. Regulation of superoxide dismutase expression by copper availability［J］. Physiologia Plantarum，2007，129(4)：747-755.
［4］Puig S，Thiele D J. Molecular mechanisms of copper uptake and distribution［J］. Current Opinion in Chemical Biology，2002，6(2)：171-180.
［5］De Freitas J，Wintz H，Kim J H，et al. Yeast，a model organism for iron and copper metabolism studies［J］. BioMetals，2003，16(1)：185-197.
［6］Wintz H，Fox T，Wu Y Y，et al. Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal novel transporters involved in metal homeostasis［J］. J Biol Chem，2003，278(48)：47644-47653.
［7］Mukherjee I，Campbell N H，Ash J S，et al. Expression profiling of the Arabidopsis ferric chelate reductase(FRO) gene family reveals differential regulation by iron and copper［J］. Planta，2006，223(6)：1178-1190.
［8］Mira H，Martínez N，Pearrubia L. Expression of a vegetative-storage-protein gene from Arabidopsis is regulated by copper，senescence and ozone［J］. Planta，2002，214(6)：939-946.
［9］Yamasaki H，Hayashi M，Fukazawa M，et al. SQUAMOSA promoter binding protein-like7 is a central regulator for copper homeostasis in Arabidopsis［J］. Plant Cell，2009，21(1)：347-361.
［10］Bernal M，Casero D，Singh V，et al. Transcriptome sequencing identifies SPL7-regulated copper acquisition genes FRO4/FRO5 and the copper dependence of iron homeostasis in Arabidopsis［J］. Plant Cell，2012，24(2)：738-761.
［11］Cardon G，Hhmann S，Klein J，et al. Molecular characterisation of the Arabidopsis SBP-box genes［J］. Gene，1999，237(1)：91-104.

相似文献/References:

[1]武玲,陆雅萍,丁泽华,等.草菇菌糠还田对大棚土壤肥力和黄瓜产量的影响[J].江苏农业科学,2013,41(05):372.
　Wu Ling,et al.Effects of mushroom substrate return to fields on soil fertility and cucumber yield in greenhouses[J].Jiangsu Agricultural Sciences,2013,41(12):372.
[2]徐强,耿友玲,齐晓花,等.不同栽培环境下黄瓜果实单宁含量主基因-多基因遗传分析[J].江苏农业科学,2014,42(12):194.
　Xu Qiang,et al.Genetic analysis of tannin content in cucumber by mixed model of major gene and polygene under different cultural environments[J].Jiangsu Agricultural Sciences,2014,42(12):194.
[3]董晓娅,邱白晶,管贤平.电化学分析方法检测黄瓜中残留的西维因[J].江苏农业科学,2014,42(11):337.
　Dong Xiaoya,et al(7).Detection of carbaryl residues in cucumber by electrochemical method[J].Jiangsu Agricultural Sciences,2014,42(12):337.
[4]王素平,孙艳军.H2O2预处理对低温下黄瓜幼苗抗氧化酶同工酶的影响[J].江苏农业科学,2013,41(06):123.
　Wang Suping,et al.Effect of H2O2 pretreatment on antioxidant isoenzyme in cucumber seedlings under chilling stress[J].Jiangsu Agricultural Sciences,2013,41(12):123.
[5]田福发,陈立昶,姜若勇,等.内置式秸秆反应堆对日光温室番茄和黄瓜生长的影响[J].江苏农业科学,2013,41(09):143.
　Tian Fufa,et al.Effect of built-in straw bio-reactor on growth of tomato and cucumber in greenhouse[J].Jiangsu Agricultural Sciences,2013,41(12):143.
[6]霍艳林,鲁顺保,关正君.盐胁迫对晋南部分主栽黄瓜品种种子萌发特性的影响[J].江苏农业科学,2016,44(03):165.
　Huo Yanlin,et al.Effect of salt stress on seed germination characteristics of cucumber cultivars in southern Shanxi[J].Jiangsu Agricultural Sciences,2016,44(12):165.
[7]王素平.钙和水杨酸对低温下黄瓜幼苗抗氧化酶同工酶的影响[J].江苏农业科学,2016,44(03):168.
　Wang Suping.Effects of Ca2+ and SA pretreatment on antioxidase isoforms in cucumber seedlings under chilling stress[J].Jiangsu Agricultural Sciences,2016,44(12):168.
[8]高攀,郭世荣,阳燕娟,等.Ca(NO3)2胁迫对白籽南瓜嫁接黄瓜幼苗生长及膜透性的影响[J].江苏农业科学,2013,41(11):157.
　Gao Pan,et al.Effects of Ca(NO3)2 stress on growth and cell membrane permeability of white seed pumpkin grafted cucumber seedlings[J].Jiangsu Agricultural Sciences,2013,41(12):157.
[9]王红君,张梦,赵辉,等.基于BP神经网络的温室黄瓜灌溉预测模型[J].江苏农业科学,2013,41(11):407.
　Wang Hongjun,et al.Irrigation forecast model of cucumber in greenhouse based on BP neural networks[J].Jiangsu Agricultural Sciences,2013,41(12):407.
[10]费雨兰,王晶,沈佳,等.不同砧木嫁接对黄瓜长势及果实品质的影响[J].江苏农业科学,2013,41(12):147.
　Fei Yulan,et al.Effects of different rootstocks on growth and quality of grafted cucumber[J].Jiangsu Agricultural Sciences,2013,41(12):147.

备注/Memo

备注/Memo:
收稿日期：2015-10-19
基金项目：黑龙江省青年科学基金(编号：QC2010089)。
作者简介：杜亚琳(1980—)，女，河南郾城人，硕士，实验师，研究方向为园艺作物生物技术。E-mail：duyalin123@163.com。
更新日期/Last Update: 2016-12-25