|本期目录/Table of Contents|

[1]薛存宝,范莲雪,张铅,等.黄瓜耐低氮相关基因CsAOC3的克隆及表达分析[J].江苏农业科学,2017,45(04):26-29.
　Xue Cunbao,et al.Cloning and expression analysis of low nitrogen tolerance related gene CsAOC3 in cucumber[J].Jiangsu Agricultural Sciences,2017,45(04):26-29.
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黄瓜耐低氮相关基因CsAOC3的克隆及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年04期
页码:
26-29
栏目:
生物技术
出版日期:
2017-02-20

文章信息/Info

Title:
Cloning and expression analysis of low nitrogen tolerance related gene CsAOC3 in cucumber
作者:
薛存宝; 范莲雪; 张铅; 王悦; 郭冬雪; 秦智伟; 武涛
东北农业大学园艺学院，黑龙江哈尔滨 150030
Author(s):
Xue Cunbao; et al
关键词:
黄瓜; 耐低氮; CsAOC3基因; 克隆; 表达分析
Keywords:
-
分类号:
Q786
DOI:
-
文献标志码:
A
摘要:
从黄瓜叶片中分离获得1个丙二烯氧化物环化酶基因，命名为CsAOC3。该基因cDNA全长为753 bp，编码250个氨基酸，与甜瓜的丙二烯氧化物环化酶(AOC)序列同源性最高。通过荧光定量PCR(qRT-PCR)分析发现，CsAOC3在低氮胁迫条件下只有在耐低氮黄瓜品系D0328中的表达量明显上调，在耐低氮能力弱的黄瓜品系D0422中的表达量并无明显变化。研究结果可为黄瓜耐低氮的基因功能研究提供理论依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2016-01-09
基金项目：国家自然科学基金青年科学基金(编号：31101545)；黑龙江省普通本科高等学校青年创新人才培养计划(编号：UNPYSCT-2015001)；2015年黑龙江省大学生创新创业训练计划(编号：201510224015)；东北农业大学“青年才俊”项目(编号：14QC07)。
作者简介：薛存宝(1991—)，男，黑龙江依安人，硕士研究生，主要从事蔬菜作物营养及抗逆分子生物学研究。E-mail：flyingnowings@163.com。
通信作者：武涛，博士，副教授，从事蔬菜作物营养及抗逆分子生物学研究。Tel：(0451)55190214；E-mail：wutao@neau.edu.cn。
更新日期/Last Update: 2017-02-20