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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第45卷

期数:

2017年05期

页码:

157-160

栏目:

畜牧兽医与水产蚕桑

出版日期:

2017-03-05

文章信息/Info

Title:

Establishment of quantitative detection method for plasma oxytocin level in sheep

作者:

席丽¹; 闫永锋¹; 秦新喜²; 韩进诚¹; 瞿红侠¹; 张进良¹

1.商丘师范学院，河南商丘 476000； 2.普莱柯生物工程股份有限公司，河南洛阳 471000

Author(s):

Xi Li; et al

关键词:

高效液相色谱-串联质谱; 多反应监测; 乙腈沉降法; 催产素; 血浆

Keywords:

-

分类号:

S858.267.2

DOI:

-

文献标志码:

A

摘要:

建立采用高效液相色谱-串联质谱(high performance liquid chromatography-tandem mass spectrometry，HPLC-MS/MS)测定绵羊血浆中催产素(oxytocin，OT)的方法。经乙腈沉降法提取血浆样品，在多反应离子监测(multiple reaction monitoring，MRM)模式下进行检测，标准加入法定量。通过该方法获得标准曲线线性方程为y=1.448x+544.400，相关系数为0.998，线性范围在50～10 000 pg/mL，方法检出限为10 pg/mL，定量限为50 pg/mL。应用 HPLC-MS/MS法测定醋酸去甲雄三烯醇酮-17β雌二醇(trenbolone acetate and estradiol-17β，TBA-E₂)联合处理阉公羊血浆中OT浓度变化，其中对照组催产素浓度为55～80 pg/mL，TBA-E₂处理组为75～160 pg/mL，表明TBA-E₂可提高阉公羊血浆中的OT浓度。该方法简便、快速、准确，可用于血浆样品中OT的检测。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2016-01-04
基金项目：商丘师范学院博士科研启动经费。
作者简介：席丽(1982—)，女，天津人，博士，讲师，从事家畜生殖与内分泌学研究。E-mail：xili_0808@163.com。

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更新日期/Last Update: 2017-03-05